Restriction map for
pFN21AE0480
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI BstYI Cac8I CelII
Cfr10I CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HaeIII HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MflI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1357 GTATAC GT^[AC][GT]AC
AflIII 263 ACATGT A^C[GA][CT]GT
AhdI 816 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 520 GTGCAC G^TGCAC
AlwI 904 GATCC GGATC(4/5)
AocI 1336 CCTCAGG CC^T[ACGT]AGG
ApaLI 520 GTGCAC G^TGCAC
AspEI 816 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 520 GTGCAC G[AT]GC[AT]^C
BmyI 520 GTGCAC G[AGT]GC[ACT]^C
BpmI 1010 CTGGAG CTGGAG(16/14)
BsaJI 482 CCGAGG C^C[ACGT][ACGT]GG
BsiHKAI 520 GTGCAC G[AT]GC[AT]^C
BsmFI 1238 GGGAC GGGAC(10/14)
Bsp1286I 520 GTGCAC G[AGT]GC[ACT]^C
BspDI 449 ATCGAT AT^CGAT
BspHI 1074 TCATGA T^CATGA
BspLU11I 263 ACATGT A^CATGT
BssKI 144 CCTGG ^CC[ACGT]GG
Bst1107I 1357 GTATAC GTA^TAC
BstAPI 1279 GCATGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 144 CCTGG CC^[AT]GG
BstZ17I 1357 GTATAC GTA^TAC
Bsu36I 1336 CCTCAGG CC^T[ACGT]AGG
CfoI 801 GCGC GCG^C
ClaI 449 ATCGAT AT^CGAT
Eam1105I 816 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 571 GAAGAG CTCTTC(1/4)
Eco81I 1336 CCTCAGG CC^T[ACGT]AGG
EcoRII 144 CCTGG ^CC[AT]GG
EcoT22I 755 ATGCAT ATGCA^T
HhaI 801 GCGC GCG^C
HinP1I 801 GCGC G^CGC
HincII 739 GTTGAC GT[CT]^[GA]AC
HindII 739 GTTGAC GT[CT]^[GA]AC
Ksp632I 571 GAAGAG CTCTTC(1/4)
MaeIII 1294 GTGAC ^GT[ACGT]AC
MslI 379 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1112 CAGCTG C[AC]G^C[GT]G
MvaI 144 CCTGG CC^[AT]GG
NlaIV 1331 GGACCC GG[ACGT]^[ACGT]CC
NsiI 755 ATGCAT ATGCA^T
PflMI 132 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1383 GAGTC GAGTC(4/5)
PvuII 1112 CAGCTG CAG^CTG
RcaI 1074 TCATGA T^CATGA
ScaI 1223 AGTACT AGT^ACT
ScrFI 144 CCTGG CC^[ACGT]GG
SspI 461 AATATT AAT^ATT
TfiI 857 GAATC G^A[AT]TC
Tsp45I 1294 GTGAC ^GT[GC]AC
Van91I 132 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1036 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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BbsI 346 GTCTTC GAAGAC(2/6)
824 GTCTTC
BfaI 508 CTAG C^TAG
1037 CTAG
BpuAI 346 GTCTTC GAAGAC(2/6)
824 GTCTTC
BsmAI 814 GAGAC GTCTC(1/5)
1257 GTCTC
Bsp1407I 266 TGTACA T^GTACA
300 TGTACA
BsrGI 266 TGTACA T^GTACA
300 TGTACA
BstF5I 384 GGATG GGATG(2/0)
885 GGATG
FokI 384 GGATG GGATG(9/13)
885 GGATG
HinfI 857 GAATC G^A[ACGT]TC
1383 GAGTC
HphI 486 GGTGA GGTGA(8/7)
179 TCACC
MaeI 508 CTAG C^TAG
1037 CTAG
MfeI 1242 CAATTG C^AATTG
1273 CAATTG
MunI 1242 CAATTG C^AATTG
1273 CAATTG
NspI 263 ACATGT [GA]CATG^[CT]
1279 GCATGT
SspBI 266 TGTACA T^GTACA
300 TGTACA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
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AcsI AfaI AluI ApoI AvaII
BbvI BsiYI BslI BsmI BsrI
Cfr13I DdeI DpnII Fnu4HI ItaI
MboI MboII MnlI MseI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
SfaNI SfcI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I