MANEA ORF clone pFN21AE0452

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Halotag ORF Clone pFN21AE0452

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Clone info.

Species

human

Clone Name

pFN21AE0452 Sequence (with vector) Restriction Map

Product ID

FHC05914

Flexi Clone PID

FXC05914

ORF Sequence

DNA sequence (1386 bp)
Protein sequence (462 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528174]

Gene

KIEE0452

Symbol

MANEA

Alias : ENDO, hEndo

Description

mannosidase, endo-alpha

Original Clone ID

oc01152

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1386 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 462 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5032.1 2.1e-206 99.8 MANEA CCDS44110.1 3.7e-120 57.3 MANEAL CCDS426.1 9e-67 68.4 MANEAL CCDS44111.1 3.5e-48 47.6 MANEAL The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition None - - - - -
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 8 TCIILALFILFIFSLMMGLKML 29 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005267204 462 612327 99.8 100.0 NP_078917 462 612327 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0452 Download

>KIEE0452  1386 bp
ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTC
TCTCTGATGATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTT
GGACTTGACCTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGAT
TTCCAAAAGAGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAA
ATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAAC
AATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATAT
ATACATTGGAATCATCCAGTGTTAGGGCATTGGGACCCTAGAATAGCCAAGAATTATCCA
CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGT
TACAGTTCTCGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCA
ATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCT
ACTGATAACTTGGTACCCACTATTTTGGATAAAGCTCATAAATATAACCTAAAGGTTACT
TTTCACATAGAACCATATAGCAATCGAGATGATCAAAACATGTACAAAAATGTCAAGTAT
ATTATAGACAAATATGGAAATCATCCGGCCTTTTACAGGTACAAGACGAAGACTGGCAAT
GCTCTTCCTATGTTTTATGTCTATGATTCCTATATTACCAAGCCTGAAAAATGGGCCAAT
CTGTTAACCACCTCAGGGTCTCGGAGTATTCGCAATTCTCCTTATGATGGACTGTTTATT
GCCCTTCTGGTAGAAGAAAAACATAAGTATGATATTCTTCAAAGTGGTTTTGATGGAATT
TACACATATTTTGCCACAAATGGCTTTACTTATGGCTCATCACATCAGAATTGGGCTAGC
CTAAAATTATTTTGTGATAAATACAACTTAATATTTATCCCAAGTGTGGGCCCAGGATAC
ATAGATACCAGCATCCGTCCATGGAACACGCAAAACACTCGGAACCGAATCAATGGGAAG
TATTATGAAATTGGTCTGAGTGCCGCACTTCAGACACGCCCCAGCTTAATTTCTATCACC
TCTTTTAATGAGTGGCATGAAGGAACTCAGATTGAAAAAGCTGTTCCCAAAAGAACCAGT
AATACAGTGTACCTAGATTACCGTCCTCATAAACCAGGTCTTTACCTAGAACTGACTCGC
AAGTGGTCTGAAAAATACAGTAAGGAAAGAGCAACTTATGCATTAGATCGCCAGCTGCCT
GTTTCT

ORF Protein

Cloned ORF protein sequence for pFN21AE0452 Download

>KIEE0452  462 aa
MAKFRRRTCIILALFILFIFSLMMGLKMLRPNTATFGAPFGLDLLPELHQRTIHLGKNFD
FQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYSWYGNPQFDGKY
IHWNHPVLGHWDPRIAKNYPQGRHNPPDDIGSSFYPELGSYSSRDPSVIETHMRQMRSAS
IGVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLKVTFHIEPYSNRDDQNMYKNVKY
IIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPEKWANLLTTSGSRSIRNSPYDGLFI
ALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSSHQNWASLKLFCDKYNLIFIPSVGPGY
IDTSIRPWNTQNTRNRINGKYYEIGLSAALQTRPSLISITSFNEWHEGTQIEKAVPKRTS
NTVYLDYRPHKPGLYLELTRKWSEKYSKERATYALDRQLPVS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0452 Download

>pFN21AE0452  6091 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTT
GGCACTTTTTATTCTATTTATTTTCTCTCTGATGATGGGTTTAAAAATGCTGAGACCAAA
TACAGCTACTTTTGGAGCTCCTTTTGGACTTGACCTTCTTCCAGAACTTCATCAACGAAC
TATTCATTTGGGGAAAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAAACAAA
TACCAAGAATTTAAAAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAA
CTTGGATGAACTACCACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGG
AAATCCACAATTTGATGGTAAATATATACATTGGAATCATCCAGTGTTAGGGCATTGGGA
CCCTAGAATAGCCAAGAATTATCCACAAGGGAGACACAACCCTCCAGATGACATTGGCTC
CAGCTTTTATCCTGAATTGGGAAGTTACAGTTCTCGGGATCCTTCTGTCATAGAAACTCA
CATGAGACAAATGCGCTCAGCTTCAATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGA
TGTAAATGATGAAAATGGAGAACCTACTGATAACTTGGTACCCACTATTTTGGATAAAGC
TCATAAATATAACCTAAAGGTTACTTTTCACATAGAACCATATAGCAATCGAGATGATCA
AAACATGTACAAAAATGTCAAGTATATTATAGACAAATATGGAAATCATCCGGCCTTTTA
CAGGTACAAGACGAAGACTGGCAATGCTCTTCCTATGTTTTATGTCTATGATTCCTATAT
TACCAAGCCTGAAAAATGGGCCAATCTGTTAACCACCTCAGGGTCTCGGAGTATTCGCAA
TTCTCCTTATGATGGACTGTTTATTGCCCTTCTGGTAGAAGAAAAACATAAGTATGATAT
TCTTCAAAGTGGTTTTGATGGAATTTACACATATTTTGCCACAAATGGCTTTACTTATGG
CTCATCACATCAGAATTGGGCTAGCCTAAAATTATTTTGTGATAAATACAACTTAATATT
TATCCCAAGTGTGGGCCCAGGATACATAGATACCAGCATCCGTCCATGGAACACGCAAAA
CACTCGGAACCGAATCAATGGGAAGTATTATGAAATTGGTCTGAGTGCCGCACTTCAGAC
ACGCCCCAGCTTAATTTCTATCACCTCTTTTAATGAGTGGCATGAAGGAACTCAGATTGA
AAAAGCTGTTCCCAAAAGAACCAGTAATACAGTGTACCTAGATTACCGTCCTCATAAACC
AGGTCTTTACCTAGAACTGACTCGCAAGTGGTCTGAAAAATACAGTAAGGAAAGAGCAAC
TTATGCATTAGATCGCCAGCTGCCTGTTTCTGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT