Restriction map for
pFN21AE0452
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KspI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AciI 1163 CCGC CCGC(-3/-1)
AocI 851 CCTCAGG CC^T[ACGT]AGG
ApaI 1068 GGGCCC GGGCC^C
AspHI 110 GAGCTC G[AT]GC[AT]^C
AvaI 488 CTCGGG C^[CT]CG[GA]G
AvaII 393 GGACC G^G[AT]CC
BamHI 492 GGATCC G^GATCC
BbsI 768 GAAGAC GAAGAC(2/6)
BbvI 1374 GCTGC GCAGC(8/12)
BcgI 1110 GCAAAACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1075 GGATAC GTATCC(6/5)
BclI 690 TGATCA T^GATCA
BlpI 530 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 530 GCTCAGC GC^T[ACGT]AGC
BpuAI 768 GAAGAC GAAGAC(2/6)
BsiHKAI 110 GAGCTC G[AT]GC[AT]^C
BsmFI 392 GGGAC GGGAC(10/14)
BsoBI 488 CTCGGG C^[CT]CG[GA]G
Bsp1407I 701 TGTACA T^GTACA
BsrDI 776 GCAATG GCAATG(2/0)
BsrGI 701 TGTACA T^GTACA
BstAPI 1351 GCAACTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 617 CCACTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 492 GGATCC [GA]^GATC[CT]
Bsu36I 851 CCTCAGG CC^T[ACGT]AGG
CelII 530 GCTCAGC GC^T[ACGT]AGC
CfoI 528 GCGC GCG^C
DraII 392 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1099 CCATGG C^C[GA][CT]GG
EarI 782 CTCTTC CTCTTC(1/4)
Eco81I 851 CCTCAGG CC^T[ACGT]AGG
EcoNI 749 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 392 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1099 CCATGG C^C[AT][AT]GG
EcoT22I 1358 ATGCAT ATGCA^T
FbaI 690 TGATCA T^GATCA
HapII 745 CCGG C^CGG
HhaI 528 GCGC GCG^C
HinP1I 528 GCGC G^CGC
HincII 843 GTTAAC GT[CT]^[GA]AC
HindII 843 GTTAAC GT[CT]^[GA]AC
HpaI 843 GTTAAC GTT^AAC
HpaII 745 CCGG C^CGG
HphI 1196 TCACC GGTGA(8/7)
Ksp632I 782 CTCTTC CTCTTC(1/4)
MfeI 539 CAATTG C^AATTG
MflI 492 GGATCC [GA]^GATC[CT]
MslI 922 CATAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1372 CAGCTG C[AC]G^C[GT]G
MspI 745 CCGG C^CGG
MunI 539 CAATTG C^AATTG
MwoI 1351 GCAACTTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1099 CCATGG C^CATGG
NheI 1015 GCTAGC G^CTAGC
NsiI 1358 ATGCAT ATGCA^T
PleI 1314 GACTC GAGTC(4/5)
PpuMI 392 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1372 CAGCTG CAG^CTG
SacI 110 GAGCTC GAGCT^C
SapI 781 GCTCTTC GCTCTTC(1/4)
SexAI 1293 ACCAGGT A^CC[AT]GGT
SspBI 701 TGTACA T^GTACA
SspI 1050 AATATT AAT^ATT
StyI 1099 CCATGG C^C[AT][AT]GG
TaqI 684 TCGA T^CGA
TseI 1374 GCTGC G^C[AT]GC
Tsp45I 191 GTGAC ^GT[GC]AC
TthHB8I 684 TCGA T^CGA
XhoII 492 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 563 GGTACC G^GTACC
612 GGTACC
AflIII 309 ACATGT A^C[GA][CT]GT
698 ACATGT
AlwI 493 GATCC GGATC(4/5)
492 GGATC
Asp718I 563 GGTACC G^GTACC
612 GGTACC
BanI 563 GGTACC G^G[CT][GA]CC
612 GGTACC
BanII 110 GAGCTC G[GA]GC[CT]^C
1068 GGGCCC
BmyI 110 GAGCTC G[AGT]GC[ACT]^C
1068 GGGCCC
BpmI 437 CTCCAG CTGGAG(16/14)
453 CTCCAG
BsaI 87 GAGACC GGTCTC(1/5)
857 GGTCTC
BsaJI 1071 CCCAGG C^C[ACGT][ACGT]GG
1099 CCATGG
Bsp1286I 110 GAGCTC G[AGT]GC[ACT]^C
1068 GGGCCC
BspLU11I 309 ACATGT A^CATGT
698 ACATGT
BssKI 1072 CCAGG ^CC[ACGT]GG
1294 CCAGG
BstNI 1072 CCAGG CC^[AT]GG
1294 CCAGG
Cac8I 1015 GCTAGC GC[ACGT]^[ACGT]GC
1370 GCCAGC
DraI 75 TTTAAA TTT^AAA
225 TTTAAA
EcoRII 1072 CCAGG ^CC[AT]GG
1294 CCAGG
Fnu4HI 1162 GCCGC GC^[ACGT]GC
1374 GCTGC
ItaI 1162 GCCGC GC^[ACGT]GC
1374 GCTGC
KpnI 563 GGTACC GGTAC^C
612 GGTACC
MvaI 1072 CCAGG CC^[AT]GG
1294 CCAGG
NspI 309 ACATGT [GA]CATG^[CT]
698 ACATGT
ScrFI 1072 CCAGG CC^[ACGT]GG
1294 CCAGG
SfaNI 26 GCATC GCATC(5/9)
1091 GCATC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsiYI BslI BsmAI BsrI BstF5I
Cfr13I DdeI DpnII FokI HaeIII
HinfI MaeI MaeIII MboI MboII
MnlI MseI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I TfiI Tru9I
Tsp509I TspRI