(Circular) (Six-base) MAPSORT of: pFN21AE0452.seq Check: 1434 from: 1 to: 6091 pFN21AE0452 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 2568 2617 3412 2568 Size: 49 795 5247 Fragments arranged by size: 5247 795 49 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3158 4154 5538 5806 1632 Size: 1526 996 1384 268 1917 Fragments arranged by size: 1917 1526 1384 996 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2314 2703 4990 5967 1203 Size: 1111 389 2287 977 1327 Fragments arranged by size: 2287 1327 1111 977 389 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaI G_GGCC'C Cuts at: 3077 3077 Size: 6091 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2176 2227 2961 3400 3626 3778 5887 1493 Size: 683 51 734 439 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 683 439 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2493 3416 1870 Size: 73 550 923 4545 Fragments arranged by size: 4545 923 550 73 BamHI G'GATC_C Cuts at: 2497 3421 3788 2497 Size: 924 367 4800 Fragments arranged by size: 4800 924 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2568 2617 3412 4867 618 Size: 325 217 627 781 49 795 1455 1842 Fragments arranged by size: 1842 1455 795 781 627 325 217 49 BanII G_rGCy'C Cuts at: 1391 2119 3077 3410 1391 Size: 728 958 333 4072 Fragments arranged by size: 4072 958 728 333 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2780 928 Size: 971 881 4239 Fragments arranged by size: 4239 971 881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3104 3138 4282 4316 1903 Size: 34 1167 34 1144 34 3678 Fragments arranged by size: 3678 1167 1144 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3074 3983 5193 3074 Size: 909 1210 3972 Fragments arranged by size: 3972 1210 909 BclI T'GATC_A Cuts at: 1548 2695 1548 Size: 1147 4944 BfrBI ATG'CAT Cuts at: 3365 3365 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2536 3508 2536 Size: 972 5119 Bme1580I G_kGCm'C Cuts at: 1790 3077 4152 5308 1790 Size: 1287 1075 1156 2573 Fragments arranged by size: 2573 1287 1156 1075 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3024 1056 Size: 1968 4123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 2443 4751 1239 Size: 1188 16 2308 2579 Fragments arranged by size: 2579 2308 1188 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2086 2868 4754 882 Size: 617 587 782 1886 2219 Fragments arranged by size: 2219 1886 782 617 587 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 4798 Fragments arranged by size: 4798 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6091 BseYI C'CCAG_C Cuts at: 3185 5294 5790 3185 Size: 2109 496 3486 Fragments arranged by size: 3486 2109 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2119 4152 4237 5308 2119 Size: 2033 85 1071 2902 Fragments arranged by size: 2902 2033 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 3077 3410 4152 4237 5308 1391 Size: 399 329 958 333 742 85 1071 2174 Fragments arranged by size: 2174 1071 958 742 399 333 329 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2788 4586 4760 65 Size: 1183 1540 1798 174 1396 Fragments arranged by size: 1798 1540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 3362 794 Size: 2568 3523 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstXI CCAn_nnnn'nTGG Cuts at: 2629 2629 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2497 3421 3788 4173 4190 5642 5728 5740 Size: 525 924 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 924 525 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2857 2857 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2004 3104 513 Size: 552 939 1100 3500 Fragments arranged by size: 3500 1100 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 2082 2232 3395 2082 Size: 150 1163 4778 Fragments arranged by size: 4778 1163 150 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056 Size: 54 1425 50 275 18 1708 889 1629 Cuts at: 6056 8 Size: 43 Fragments arranged by size: 1708 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 4021 1353 Size: 1440 1228 3423 Fragments arranged by size: 3423 1440 1228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2117 2117 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 2443 3158 4154 4751 5538 5806 Size: 393 795 16 715 996 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 996 795 787 715 597 393 268 16 EcoNI CCTnn'n_nnAGG Cuts at: 2758 2758 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 2398 2398 Size: 6091 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3375 3375 Size: 6091 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 4732 4764 4806 Size: 32 135 32 869 32 2259 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 2259 869 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3435 3681 669 Size: 988 281 912 585 246 3079 Fragments arranged by size: 3079 988 912 585 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 2850 3681 2850 Size: 831 5260 KpnI G_GTAC'C Cuts at: 2572 2621 3416 2572 Size: 49 795 5247 Fragments arranged by size: 5247 795 49 MfeI C'AATT_G Cuts at: 2544 3690 2544 Size: 1146 4945 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4269 Fragments arranged by size: 4269 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2931 4049 4408 4567 518 Size: 648 1765 1118 359 159 2042 Fragments arranged by size: 2042 1765 1118 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3379 3507 4184 5332 5577 1606 Size: 1773 128 677 1148 245 2120 Fragments arranged by size: 2120 1773 1148 677 245 128 NcoI C'CATG_G Cuts at: 513 1065 2004 3104 513 Size: 552 939 1100 3500 Fragments arranged by size: 3500 1100 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 3020 1052 Size: 1968 4123 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NsiI A_TGCA'T Cuts at: 3367 3367 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2318 2707 3449 4994 1132 Size: 97 1089 389 742 1545 2229 Fragments arranged by size: 2229 1545 1089 742 389 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 2314 2703 4990 2314 Size: 389 2287 3415 Fragments arranged by size: 3415 2287 389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2398 2398 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 3073 3073 Size: 6091 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3250 3282 3250 Size: 32 6059 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 3379 3379 Size: 6091 SacI G_AGCT'C Cuts at: 2119 2119 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SanDI GG'GwC_CC Cuts at: 2398 2398 Size: 6091 SapI GCTCTTCn'nnn_ Cuts at: 2793 2793 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 3298 3298 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 3439 4574 5255 5446 826 Size: 219 2394 1135 681 191 1471 Fragments arranged by size: 2394 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 3057 4015 5 Size: 47 3005 958 2081 Fragments arranged by size: 3005 2081 958 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 3104 3530 513 Size: 552 939 1100 426 3074 Fragments arranged by size: 3074 1100 939 552 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4118 4135 4288 4473 4918 325 Size: 1255 2538 17 153 185 445 1498 Fragments arranged by size: 2538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2551 2706 4337 Size: 274 80 33 51 1443 574 155 1631 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1712 1631 1443 574 274 155 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacII SfiI SfoI SrfI SwaI XcmI