DDX47 ORF clone pFN21AB8694

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8694

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Clone info.

Species

human

Clone Name

pFN21AB8694 Sequence (with vector) Restriction Map

Product ID

FHC02943

Flexi Clone PID

FXC02943

ORF Sequence

DNA sequence (1365 bp)
Protein sequence (455 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB462951]

Gene

KIBB8694

Symbol

DDX47

Alias : E4-DBP, HQ0256, MSTP162, RRP3

Description

DEAD (Asp-Glu-Ala-Asp) box polypeptide 47, transcript variant 1

Original Clone ID

oc00357

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1365 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 455 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8655.1 2.9e-186 99.8 DDX47 CCDS8656.1 5e-99 89.0 DDX47 CCDS12390.1 3.1e-74 44.4 DDX49 CCDS13416.1 8.2e-54 37.2 DDX27 CCDS8342.1 3.2e-44 33.9 DDX10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 48 215 PF00270 DNA/RNA helicase IPR001650 282 358 PF00271 Helicase HMMSmart IPR014001 43 241 SM00487 DEAD-like helicase IPR001650 277 358 SM00490 Helicase ProfileScan IPR014014 24 52 PS51195 RNA helicase IPR014001 55 226 PS51192 DEAD-like helicase IPR001650 237 397 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057439 455 615428 99.8 100.0 NP_957518 406 615428 89.0 100.0 NP_060365 796 616621 37.2 90.3 NP_004389 875 601235 33.9 89.7 NP_055555 411 268305,608546 31.9 81.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8694 Download

>KIBB8694  1365 bp
ATGGCGGCACCCGAGGAACACGATTCTCCGACCGAAGCGTCCCAGCCGATTGTGGAAGAG
GAGGAAACTAAAACATTTAAAGACCTGGGTGTGACAGATGTGTTGTGTGAAGCTTGTGAC
CAGTTGGGATGGACAAAACCCACCAAGATTCAGATTGAAGCTATTCCTTTGGCCTTACAA
GGTCGTGATATCATTGGGCTTGCAGAAACTGGCCCTGGAAAGACAGGCGCCTTTGCTTTG
CCCATTCTAAACGCACTGCTGGAGACCCCGCAGCGTTTGTTTGCCCTAGTTCTTACCCCG
ACTCGGGAGCTGGCCTTTCAGATCTCAGAGCAGTTTGAAGCCCTGGGGTCCTCTATTGGA
GTGCAGAGTGCTGTGATTGTAGGTGGAATTGATTCAATGTCTCAATCTTTGGCCCTTGCA
AAAAAACCACATATAATAATAGCAACTCCTGGTCGACTGATTGACCACTTGGAAAATACG
AAAGGTTTCAACTTGAGAGCTCTCAAATACTTGGTCATGGATGAAGCCGACCGAATACTG
AATATGGATTTTGAGACAGAGGTTGACAAGATCCTCAAAGTGATTCCTCGAGATCGGAAA
ACATTCCTCTTCTCTGCCACCATGACCAAGAAGGTTCAAAAACTTCAGCGAGCAGCTCTG
AAGAATCCTGTGAAATGTGCCGTTTCCTCTAAATACCAGACAGTTGAAAAATTACAGCAA
TATTATATTTTTATTCCCTCTAAATTCAAGGATACCTACCTGGTTTATATTCTAAATGAA
TTGGCTGGAAACTCCTTTATGATATTCTGCAGCACCTGTAATAATACCCAGAGAACAGCT
TTGCTACTGCGAAATCTTGGCTTCACTGCCATCCCCCTCCATGGACAAATGAGTCAGAGT
AAGCGCCTAGGATCCCTTAATAAGTTTAAGGCCAAGGCCCGTTCCATTCTTCTAGCAACT
GACGTTGCCAGCCGAGGTTTGGACATACCTCATGTAGATGTGGTTGTCAACTTTGACATT
CCTACCCATTCCAAGGATTACATCCATCGAGTAGGTCGAACAGCTAGAGCTGGGCGCTCC
GGAAAGGCTATTACTTTTGTCACACAGTATGATGTGGAACTCTTCCAGCGCATAGAACAC
TTAATTGGGAAGAAACTACCAGGTTTTCCAACACAGGATGATGAGGTTATGATGCTGACA
GAACGCGTCGCTGAAGCCCAAAGGTTTGCCCGAATGGAGTTAAGGGAGCATGGAGAAAAG
AAGAAACGCTCGCGAGAGGATGCTGGAGATAATGATGACACAGAGGGTGCTATTGGTGTC
AGGAACAAGGTGGCTGGAGGAAAAATGAAGAAGCGGAAAGGCCGT

ORF Protein

Cloned ORF protein sequence for pFN21AB8694 Download

>KIBB8694  455 aa
MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQ
GRDIIGLAETGPGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIG
VQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRIL
NMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQ
YYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQS
KRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRS
GKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEK
KKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8694 Download

>pFN21AB8694  6070 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCACCCGAGGAACACGATTCTCCGACCGA
AGCGTCCCAGCCGATTGTGGAAGAGGAGGAAACTAAAACATTTAAAGACCTGGGTGTGAC
AGATGTGTTGTGTGAAGCTTGTGACCAGTTGGGATGGACAAAACCCACCAAGATTCAGAT
TGAAGCTATTCCTTTGGCCTTACAAGGTCGTGATATCATTGGGCTTGCAGAAACTGGCCC
TGGAAAGACAGGCGCCTTTGCTTTGCCCATTCTAAACGCACTGCTGGAGACCCCGCAGCG
TTTGTTTGCCCTAGTTCTTACCCCGACTCGGGAGCTGGCCTTTCAGATCTCAGAGCAGTT
TGAAGCCCTGGGGTCCTCTATTGGAGTGCAGAGTGCTGTGATTGTAGGTGGAATTGATTC
AATGTCTCAATCTTTGGCCCTTGCAAAAAAACCACATATAATAATAGCAACTCCTGGTCG
ACTGATTGACCACTTGGAAAATACGAAAGGTTTCAACTTGAGAGCTCTCAAATACTTGGT
CATGGATGAAGCCGACCGAATACTGAATATGGATTTTGAGACAGAGGTTGACAAGATCCT
CAAAGTGATTCCTCGAGATCGGAAAACATTCCTCTTCTCTGCCACCATGACCAAGAAGGT
TCAAAAACTTCAGCGAGCAGCTCTGAAGAATCCTGTGAAATGTGCCGTTTCCTCTAAATA
CCAGACAGTTGAAAAATTACAGCAATATTATATTTTTATTCCCTCTAAATTCAAGGATAC
CTACCTGGTTTATATTCTAAATGAATTGGCTGGAAACTCCTTTATGATATTCTGCAGCAC
CTGTAATAATACCCAGAGAACAGCTTTGCTACTGCGAAATCTTGGCTTCACTGCCATCCC
CCTCCATGGACAAATGAGTCAGAGTAAGCGCCTAGGATCCCTTAATAAGTTTAAGGCCAA
GGCCCGTTCCATTCTTCTAGCAACTGACGTTGCCAGCCGAGGTTTGGACATACCTCATGT
AGATGTGGTTGTCAACTTTGACATTCCTACCCATTCCAAGGATTACATCCATCGAGTAGG
TCGAACAGCTAGAGCTGGGCGCTCCGGAAAGGCTATTACTTTTGTCACACAGTATGATGT
GGAACTCTTCCAGCGCATAGAACACTTAATTGGGAAGAAACTACCAGGTTTTCCAACACA
GGATGATGAGGTTATGATGCTGACAGAACGCGTCGCTGAAGCCCAAAGGTTTGCCCGAAT
GGAGTTAAGGGAGCATGGAGAAAAGAAGAAACGCTCGCGAGAGGATGCTGGAGATAATGA
TGACACAGAGGGTGCTATTGGTGTCAGGAACAAGGTGGCTGGAGGAAAAATGAAGAAGCG
GAAAGGCCGTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT