Restriction map for
pFN21AB8694
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AclI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BalI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HpaI
HphI KpnI KspI MamI MfeI
MluNI MscI MspA1I MunI MwoI
NaeI NciI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacII
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 452 GTCGAC GT^[AC][GT]AC
AccIII 1078 TCCGGA T^CCGGA
AcsI 742 AAATTC [GA]^AATT[CT]
AcyI 226 GGCGCC G[GA]^CG[CT]C
AflIII 1203 ACGCGT A^C[GA][CT]GT
ApoI 742 AAATTC [GA]^AATT[CT]
AspHI 497 GAGCTC G[AT]GC[AT]^C
AvaII 347 GGTCC G^G[AT]CC
AvrII 906 CCTAGG C^CTAGG
BamHI 910 GGATCC G^GATCC
BanII 497 GAGCTC G[GA]GC[CT]^C
BbeI 226 GGCGCC GGCGC^C
BciVI 750 GGATAC GTATCC(6/5)
BglII 320 AGATCT A^GATCT
BlnI 906 CCTAGG C^CTAGG
BmyI 497 GAGCTC G[AGT]GC[ACT]^C
BsaHI 226 GGCGCC G[GA]^CG[CT]C
BsaI 262 GAGACC GGTCTC(1/5)
BsaWI 1078 TCCGGA [AT]^CCGG[AT]
BseAI 1078 TCCGGA T^CCGGA
BseRI 58 GAGGAG GAGGAG(10/8)
BsgI 361 GTGCAG GTGCAG(16/14)
BsiHKAI 497 GAGCTC G[AT]GC[AT]^C
BsmFI 39 GTCCC GGGAC(10/14)
Bsp1286I 497 GAGCTC G[AGT]GC[ACT]^C
BspEI 1078 TCCGGA T^CCGGA
DdeI 324 CTCAG C^T[ACGT]AG
DraI 76 TTTAAA TTT^AAA
DraII 346 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 879 CCATGG C^C[GA][CT]GG
EcoO109I 346 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 187 GATATC GAT^ATC
FauI 267 CCCGC CCCGC(4/6)
HapII 1079 CCGG C^CGG
Hin1I 226 GGCGCC G[GA]^CG[CT]C
HindIII 110 AAGCTT A^AGCTT
HpaII 1079 CCGG C^CGG
KasI 226 GGCGCC G^GCGCC
MaeII 962 ACGT A^CGT
MluI 1203 ACGCGT A^CGCGT
MroI 1078 TCCGGA T^CCGGA
MslI 991 CATGTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1079 CCGG C^CGG
NarI 226 GGCGCC GG^CGCC
NcoI 879 CCATGG C^CATGG
NruI 1270 TCGCGA TCG^CGA
PaeR7I 587 CTCGAG C^TCGAG
PpuMI 346 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 807 CTGCAG CTGCA^G
SacI 497 GAGCTC GAGCT^C
SalI 452 GTCGAC G^TCGAC
SfcI 807 CTGCAG C^T[GA][CT]AG
SfoI 226 GGCGCC GGC^GCC
SspI 719 AATATT AAT^ATT
XcmI 968 CCAGCCGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 587 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 1204 CGCG CG^CG
1271 CGCG
AlwNI 203 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
810 CAGCACCTG
BanI 6 GGCACC G^G[CT][GA]CC
226 GGCGCC
BsiEI 29 CGACCG CG[GA][CT]^CG
528 CGACCG
BsrI 208 ACTGG ACTGG(1/-1)
120 CCAGT
BstUI 1204 CGCG CG^CG
1271 CGCG
DrdI 30 GACCGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
884 GACAAATGAGTC
HgaI 37 GCGTC GACGC(5/10)
1205 GCGTC
MvnI 1204 CGCG CG^CG
1271 CGCG
PleI 300 GACTC GAGTC(4/5)
891 GAGTC
SexAI 758 ACCTGGT A^CC[AT]GGT
1158 ACCAGGT
SfaNI 1191 GATGC GCATC(5/9)
1279 GATGC
SmlI 492 CTTGAG C^T[CT][GA]AG
587 CTCGAG
TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^
864 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI Asp700I AvaI
BbvI BfaI BpmI BsaJI BsiYI
BslI BsmAI BsoBI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TthHB8I XhoII XmnI