PLK3 ORF clone pFN21AB6158

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB6158

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Clone info.

Species

human

Clone Name

pFN21AB6158 Sequence (with vector) Restriction Map

Product ID

FHC11753

ORF Sequence

DNA sequence (1938 bp)
Protein sequence (646 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB6158

Symbol

PLK3

Alias : CNK, FNK, PLK-3, PRK

Description

polo-like kinase 3

Original Clone ID

cs00758

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1938 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 646 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS515.1 7.8e-149 100.0 PLK3 CCDS75250.1 1.1e-47 49.6 PLK2 CCDS3974.1 1.6e-47 50.6 PLK2 CCDS10616.1 4.1e-35 40.7 PLK1 CCDS3735.1 3.4e-21 37.4 PLK4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 64 314 PF00069 Serine/threonine-protein kinase-like domain IPR000959 470 532 PF00659 POLO box duplicated domain IPR000959 568 636 PF00659 POLO box duplicated domain HMMSmart IPR002290 62 314 SM00220 Serine/threonine-protein kinase domain IPR020635 62 314 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 62 314 PS50011 Protein kinase IPR000959 470 533 PS50078 POLO box duplicated domain IPR000959 567 637 PS50078 POLO box duplicated domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004064 646 602913 100.0 100.0 NP_001239155 671 607023 49.6 99.4 NP_006613 685 607023 50.6 96.6 NP_005021 603 602098 40.7 97.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6158 Download

>KIBB6158  1938 bp
ATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCC
GCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATG
CTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGC
ACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACT
GACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTCGCCAAG
CCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCACCGC
CACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG
CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCA
GAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGC
ATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAG
GTGGGGGATTTTGGGCTGGCAGCCCGATTGGAGCCTCCGGAGCAGAGGAAGAAGACCATC
TGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCTGAG
GCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTT
GAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT
GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGA
GACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCC
GATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCT
AGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAAT
CATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTT
GGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGCCTG
GTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA
TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAAT
TGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCT
CTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTG
TCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCCAAC
AGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGTGCT
GTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATGGAG
CAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG
CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGC
ACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCC
CTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTT
CGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTGCTC
CGGGACCGCAGCCCAGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB6158 Download

>KIBB6158  646 aa
MEPAAGFLSPRPFQRAAAAPAPPAGPGPPPSALRGPELEMLAGLPTSDPGRLITDPRSGR
TYLKGRLLGKGGFARCYEATDTETGSAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHR
HIVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRG
ILHRDLKLGNFFITENMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHGPE
ADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPR
DRPSIDQILRHDFFTKGYTPDRLPISSCVTVPDLTPPNPARSLFAKVTKSLFGRKKKSKN
HAQERDEVSGLVSGLMRTSVGHQDARPEAPAASGPAPVSLVETAPEDSSPRGTLASSGDG
FEEGLTVATVVESALCALRNCIAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQL
SSRRVAVLFNDGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGILRYFASYME
QHLMKGGDLPSVEEVEVPAPPLLLQWVKTDQALLMLFSDGTVQVNFYGDHTKLILSGWEP
LLVTFVARNRSACTYLASHLRQLGCSPDLRQRLRYALRLLRDRSPA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6158 Download

>pFN21AB6158  6643 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCC
CTTCCAGCGTGCGGCCGCCGCGCCCGCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGC
CTTGCGCGGACCTGAGCTGGAGATGCTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCT
CATCACGGACCCGCGCAGCGGCCGCACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGG
CTTCGCCCGCTGCTACGAGGCCACTGACACAGAGACTGGCAGCGCCTACGCTGTCAAAGT
CATCCCGCAGAGCCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATTGA
GCTGCACCGAGACCTGCAGCACCGCCACATCGTGCGTTTTTCGCACCACTTTGAGGACGC
TGACAACATCTACATTTTCTTGGAGCTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAA
GGCCCGGCACACCCTGTTGGAGCCAGAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGG
CCTCAAGTACTTGCACCAGCGCGGCATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTT
CATCACTGAGAACATGGAACTGAAGGTGGGGGATTTTGGGCTGGCAGCCCGATTGGAGCC
TCCGGAGCAGAGGAAGAAGACCATCTGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCT
GCTGAGACAGGGCCACGGCCCTGAGGCGGATGTATGGTCACTGGGCTGTGTCATGTACAC
GCTGCTCTGCGGGAGCCCTCCCTTTGAGACGGCTGACCTGAAGGAGACGTACCGCTGCAT
CAAGCAGGTTCACTACACGCTGCCTGCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGC
CGCCATCCTTCGGGCCTCACCCCGAGACCGCCCCTCTATTGACCAGATCCTGCGCCATGA
CTTCTTTACCAAGGGCTACACCCCCGATCGACTCCCTATCAGCAGCTGCGTGACAGTCCC
AGACCTGACACCCCCCAACCCAGCTAGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTT
TGGCAGAAAGAAGAAGAGTAAGAATCATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGT
GAGCGGCCTCATGCGCACATCCGTTGGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGC
TTCTGGCCCAGCCCCTGTCAGCCTGGTAGAGACAGCACCTGAAGACAGCTCACCCCGTGG
GACACTGGCAAGCAGTGGAGATGGATTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGA
GTCAGCCCTTTGTGCTCTGAGAAATTGTATAGCCTTCATGCCCCCAGCGGAACAGAACCC
GGCCCCCCTGGCCCAGCCAGAGCCTCTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAA
TAAGTTCGGCTTTGGGTATCAACTGTCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGG
CACACATATGGCCCTGTCGGCCAACAGAAAGACTGTGCACTACAATCCCACCAGCACAAA
GCACTTCTCCTTCTCCGTGGGTGCTGTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCT
GCGGTACTTCGCCTCCTACATGGAGCAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGT
GGAAGAGGTAGAGGTACCTGCTCCGCCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGC
TCTCCTCATGCTGTTTAGTGATGGCACTGTCCAGGTGAACTTCTACGGGGACCACACCAA
GCTGATTCTCAGTGGCTGGGAGCCCCTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGC
TTGTACTTACCTCGCTTCCCACCTTCGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCG
ACTCCGCTATGCTCTGCGCCTGCTCCGGGACCGCAGCCCAGCCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT