(Circular) (Six-base) MAPSORT of: pFN21AB6158.seq Check: 9033 from: 1 to: 6643 pFN21AB6158 6643 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2144 4453 278 Size: 53 83 186 936 608 2309 2468 Fragments arranged by size: 2468 2309 936 608 186 83 53 AccI GT'mk_AC Cuts at: 1937 3986 1937 Size: 2049 4594 Acc65I G'GTAC_C Cuts at: 3673 3964 3673 Size: 291 6352 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 2819 3240 4706 6090 6358 1632 Size: 989 198 421 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 989 421 268 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 5542 6519 1203 Size: 4339 977 1327 Fragments arranged by size: 4339 1327 977 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 3293 1166 Size: 2127 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6643 AlwNI CAG_nnn'CTG Cuts at: 2255 2875 2929 2985 3182 3194 3218 3795 4386 Size: 620 54 56 197 12 24 577 591 Cuts at: 4386 5958 2255 Size: 1572 2940 Fragments arranged by size: 2940 1572 620 591 577 197 56 54 24 12 ApaI G_GGCC'C Cuts at: 2082 3577 2082 Size: 1495 5148 ApaLI G'TGCA_C Cuts at: 3515 4700 5856 3515 Size: 1185 1156 4302 Fragments arranged by size: 4302 1185 1156 ApoI r'AATT_y Cuts at: 1493 2572 3952 4178 4330 6439 1493 Size: 1079 1380 226 152 2109 1697 Fragments arranged by size: 2109 1697 1380 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 5197 160 Size: 872 4165 1606 Fragments arranged by size: 4165 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2150 2901 3570 3968 1870 Size: 73 138 69 751 669 398 4545 Fragments arranged by size: 4545 751 669 398 138 73 69 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2154 2669 3673 3964 5419 Size: 325 217 627 367 515 1004 291 1455 Cuts at: 5419 618 Size: 1842 Fragments arranged by size: 1842 1455 1004 627 515 367 325 291 217 BanII G_rGCy'C Cuts at: 1391 2082 2427 2777 3577 3804 3962 1391 Size: 691 345 350 800 227 158 4072 Fragments arranged by size: 4072 800 691 350 345 227 158 BbeI G_GCGC'C Cuts at: 2158 2158 Size: 6643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2663 3228 928 Size: 971 764 565 4343 Fragments arranged by size: 4343 971 764 565 BbvCI CC'TCA_GC Cuts at: 3585 3585 Size: 6643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2083 2117 4834 4868 1903 Size: 34 146 34 2717 34 3678 Fragments arranged by size: 3678 2717 146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4535 5745 3605 Size: 930 1210 4503 Fragments arranged by size: 4503 1210 930 BclI T'GATC_A Cuts at: 1548 1548 Size: 6643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2866 3060 3368 Size: 107 122 71 631 605 1194 194 308 Cuts at: 3368 3497 5254 136 Size: 129 1757 1525 Fragments arranged by size: 1757 1525 1194 631 605 308 194 129 122 107 71 BglII A'GATC_T Cuts at: 3646 6638 3646 Size: 2992 3651 BlpI GC'TnA_GC Cuts at: 4060 4060 Size: 6643 Bme1580I G_kGCm'C Cuts at: 1790 2082 3519 3570 3577 4704 5860 1790 Size: 292 1437 51 7 1127 1156 2573 Fragments arranged by size: 2573 1437 1156 1127 292 51 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2750 3648 4374 Size: 416 53 83 399 24 1333 898 726 Cuts at: 4374 5332 442 Size: 958 1753 Fragments arranged by size: 1753 1333 958 898 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2673 3156 3867 5303 1239 Size: 899 535 483 711 1436 2579 Fragments arranged by size: 2579 1436 899 711 535 483 Bpu10I CC'TnA_GC Cuts at: 2112 3585 2112 Size: 1473 5170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 2546 4768 5633 5931 2507 Size: 39 2222 865 298 3219 Fragments arranged by size: 3219 2222 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2343 2898 3112 5306 882 Size: 617 844 555 214 2194 2219 Fragments arranged by size: 2219 2194 844 617 555 214 BsaAI yAC'GTr Cuts at: 493 1204 4434 493 Size: 711 3230 2702 Fragments arranged by size: 3230 2702 711 BsaBI GATnn'nnATC Cuts at: 6637 6637 Size: 6643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2155 4450 4832 Size: 53 83 186 936 608 14 2295 382 Cuts at: 4832 5467 275 Size: 635 1451 Fragments arranged by size: 2295 1451 936 635 608 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 2641 3110 5076 5474 5748 5895 6306 2641 Size: 469 1966 398 274 147 411 2978 Fragments arranged by size: 2978 1966 469 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3596 3626 1178 Size: 30 165 30 2193 30 4195 Fragments arranged by size: 4195 2193 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3713 1186 Size: 2527 4116 BseYI C'CCAG_C Cuts at: 3019 3187 3343 3372 3589 3795 3871 3937 5846 Size: 168 156 29 217 206 76 66 1909 Cuts at: 5846 6342 3019 Size: 496 3320 Fragments arranged by size: 3320 1909 496 217 206 168 156 76 66 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 1163 Size: 573 591 5479 Fragments arranged by size: 5479 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 2076 2182 2968 4093 4854 Size: 275 186 54 21 106 786 1125 761 Cuts at: 4854 5882 1540 Size: 1028 2301 Fragments arranged by size: 2301 1125 1028 786 761 275 186 106 54 21 BsiHKAI G_wGCw'C Cuts at: 2427 3316 3468 3519 4704 4789 5860 2427 Size: 889 152 51 1185 85 1071 3210 Fragments arranged by size: 3210 1185 1071 889 152 85 51 BsmI GAATG_Cn' Cuts at: 4154 4247 4154 Size: 93 6550 BsmBI CGTCTCn'nnnn_ Cuts at: 2780 2798 2780 Size: 18 6625 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2427 2777 3316 3468 3519 3570 Size: 399 292 345 350 539 152 51 51 Cuts at: 3570 3577 3804 3962 4704 4789 5860 1391 Size: 7 227 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 539 399 350 345 292 227 158 152 85 51 51 7 BspEI T'CCGG_A Cuts at: 2641 6306 2641 Size: 3665 2978 BspHI T'CATG_A Cuts at: 3633 4532 6262 3633 Size: 899 1730 4014 Fragments arranged by size: 4014 1730 899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2508 2815 3685 3897 3998 844 Size: 1517 147 307 870 212 101 3489 Fragments arranged by size: 3489 1517 870 307 212 147 101 BsrBI CCG'CTC Cuts at: 1394 1823 2066 3123 4530 1394 Size: 429 243 1057 1407 3507 Fragments arranged by size: 3507 1407 1057 429 243 BsrDI GCAATG_nn' Cuts at: 65 1248 5138 5312 65 Size: 1183 3890 174 1396 Fragments arranged by size: 3890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 5287 5474 1700 Size: 110 208 3269 187 2869 Fragments arranged by size: 3269 2869 208 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 3985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 4703 5715 4703 Size: 1012 5631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 5447 5447 Size: 6643 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstXI CCAn_nnnn'nTGG Cuts at: 2683 3295 2683 Size: 612 6031 BstYI r'GATC_y Cuts at: 1972 2322 2508 2925 3646 3973 4340 4725 4742 Size: 350 186 417 721 327 367 385 17 Cuts at: 4742 6194 6280 6292 6638 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 721 417 385 367 350 346 327 186 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2721 2721 Size: 6643 BtgI C'CryG_G Cuts at: 513 1065 2004 2713 3235 3555 513 Size: 552 939 709 522 320 3601 Fragments arranged by size: 3601 939 709 552 522 320 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5904 Fragments arranged by size: 5904 683 56 BtsI GCAGTG_nn' Cuts at: 2855 3259 3704 4159 4953 4973 2855 Size: 404 445 455 794 20 4525 Fragments arranged by size: 4525 794 455 445 404 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3947 3947 Size: 6643 DraIII CAC_nnn'GTG Cuts at: 2371 3236 2371 Size: 865 5778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5650 809 Size: 507 4334 1802 Fragments arranged by size: 4334 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2073 2127 Size: 54 1425 50 275 18 222 21 54 Cuts at: 2127 2179 2877 3145 3285 3498 4090 4979 6608 Size: 52 698 268 140 213 592 889 1629 Cuts at: 6608 8 Size: 43 Fragments arranged by size: 1629 1425 889 698 592 275 268 222 213 140 54 54 52 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2052 2073 2179 4090 1537 Size: 275 240 21 106 1911 4090 Fragments arranged by size: 4090 1911 275 240 106 21 EarI CTCTTCn'nnn_ Cuts at: 1353 3068 3473 3657 4573 1353 Size: 1715 405 184 916 3423 Fragments arranged by size: 3423 1715 916 405 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2741 3672 5230 5604 5750 387 Size: 1064 138 1152 931 1558 374 146 1280 Fragments arranged by size: 1558 1280 1152 1064 931 374 146 138 EcoICRI GAG'CTC Cuts at: 2425 2425 Size: 6643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2621 2673 2819 3156 3240 3867 Size: 393 506 483 52 146 337 84 627 Cuts at: 3867 4706 5303 6090 6358 1239 Size: 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 627 597 506 483 393 337 268 146 84 52 EcoNI CCTnn'n_nnAGG Cuts at: 3635 3635 Size: 6643 EcoO109I rG'GnC_Cy Cuts at: 2557 3574 2557 Size: 1017 5626 EcoRI G'AATT_C Cuts at: 3952 4330 3952 Size: 378 6265 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2236 3073 3445 2236 Size: 837 372 5434 Fragments arranged by size: 5434 837 372 FspI TGC'GCA Cuts at: 3134 5149 3134 Size: 2015 4628 FspAI rTGC'GCAy Cuts at: 3134 3134 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 2158 2265 5790 1678 Size: 480 107 3525 2531 Fragments arranged by size: 3525 2531 480 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 2897 2929 2961 Size: 32 135 32 1148 32 145 32 32 Cuts at: 2961 2993 3067 3092 3099 3124 5284 5316 5358 Size: 32 74 25 7 25 2160 32 42 Cuts at: 5358 5390 1373 Size: 32 2626 Fragments arranged by size: 2626 2160 1148 145 135 74 42 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3409 3987 4233 669 Size: 988 281 1471 578 246 3079 Fragments arranged by size: 3079 1471 988 578 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6643 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KasI G'GCGC_C Cuts at: 2154 2154 Size: 6643 KpnI G_GTAC'C Cuts at: 3677 3968 3677 Size: 291 6352 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 5757 5941 1305 Size: 1152 3300 184 2007 Fragments arranged by size: 3300 2007 1152 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3147 3287 10 Size: 54 1425 343 1315 140 3366 Fragments arranged by size: 3366 1425 1315 343 140 54 MslI CAynn'nnrTG Cuts at: 518 1166 3153 3293 3485 4601 4960 5119 518 Size: 648 1987 140 192 1116 359 159 2042 Fragments arranged by size: 2042 1987 1116 648 359 192 159 140 MspA1I CmG'CkG Cuts at: 1606 2178 2229 2814 2985 3347 3589 3871 4059 Size: 572 51 585 171 362 242 282 188 Cuts at: 4059 4736 5884 6129 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 585 572 362 282 245 242 188 171 51 NarI GG'CG_CC Cuts at: 2155 2155 Size: 6643 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5152 Fragments arranged by size: 5152 939 552 NdeI CA'TA_TG Cuts at: 387 3486 387 Size: 3099 3544 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 2052 2179 4090 2052 Size: 127 1911 4605 Fragments arranged by size: 4605 1911 127 NspI r_CATG'y Cuts at: 1132 1229 4001 5546 1132 Size: 97 2772 1545 2229 Fragments arranged by size: 2772 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6643 PciI A'CATG_T Cuts at: 5542 5542 Size: 6643 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6643 PfoI T'CCnGG_A Cuts at: 3924 3924 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2557 2557 Size: 6643 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 4213 4213 Size: 6643 PspOMI G'GGCC_C Cuts at: 2078 3573 2078 Size: 1495 5148 PstI C_TGCA'G Cuts at: 830 2358 2432 3582 3699 3995 830 Size: 1528 74 1150 117 296 3478 Fragments arranged by size: 3478 1528 1150 296 117 74 PvuI CG_AT'CG Cuts at: 2001 2968 2001 Size: 967 5676 PvuII CAG'CTG Cuts at: 2985 3589 3871 2985 Size: 604 282 5757 Fragments arranged by size: 5757 604 282 SacI G_AGCT'C Cuts at: 2427 2427 Size: 6643 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SapI GCTCTTCn'nnn_ Cuts at: 3473 3473 Size: 6643 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 2528 4891 6603 2528 Size: 2363 1712 2568 Fragments arranged by size: 2568 2363 1712 SfcI C'TryA_G Cuts at: 826 1045 2354 2428 3578 3695 3991 5126 5807 Size: 219 1309 74 1150 117 296 1135 681 Cuts at: 5807 5998 826 Size: 191 1471 Fragments arranged by size: 1471 1309 1150 1135 681 296 219 191 117 74 SfiI GGCCn_nnn'nGGCC Cuts at: 3368 3497 3368 Size: 129 6514 SfoI GGC'GCC Cuts at: 2156 2156 Size: 6643 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 2083 2152 3572 3970 1872 Size: 211 69 1420 398 4545 Fragments arranged by size: 4545 1420 398 211 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 2561 4747 5648 5910 820 Size: 197 926 579 39 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 579 262 197 39 SnaBI TAC'GTA Cuts at: 493 4434 493 Size: 3941 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 4001 1132 Size: 2869 3774 SrfI GCCC'GGGC Cuts at: 2083 2083 Size: 6643 SspI AAT'ATT Cuts at: 5 52 4567 5 Size: 47 4515 2081 Fragments arranged by size: 4515 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6643 StyI C'CwwG_G Cuts at: 513 1065 2004 2949 4082 513 Size: 552 939 945 1133 3074 Fragments arranged by size: 3074 1133 945 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3548 4670 4687 4840 5025 5470 325 Size: 1255 1968 1122 17 153 185 445 1498 Fragments arranged by size: 1968 1498 1255 1122 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 2754 3842 Size: 274 80 33 51 1443 549 228 1088 Cuts at: 3842 4889 6601 96 Size: 1047 1712 138 Fragments arranged by size: 1712 1443 1088 1047 549 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6603 6635 6603 Size: 32 6611 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3454 3454 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 2081 2150 3570 3968 1870 Size: 211 69 1420 398 4545 Fragments arranged by size: 4545 1420 398 211 69 XmnI GAAnn'nnTTC Cuts at: 4772 4772 Size: 6643 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2142 4451 276 Size: 53 83 186 936 608 2309 2468 Fragments arranged by size: 2468 2309 936 608 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SwaI