(Linear) (Six-base) MAPSORT of: KIBB6158.seq Check: 2956 from: 1 to: 1938 KIBB6158.orf With 182 enzymes: SgfI * July 3, 2012 16:14 .. AatII G_ACGT'C Cuts at: 0 139 1938 Size: 139 1799 Acc65I G'GTAC_C Cuts at: 0 1668 1938 Size: 1668 270 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 814 1235 1938 Size: 616 198 421 703 Fragments arranged by size: 703 616 421 198 AleI CACnn'nnGTG Cuts at: 0 1288 1938 Size: 1288 650 AlwNI CAG_nnn'CTG Cuts at: 0 250 870 924 980 1177 1189 1213 1790 Size: 250 620 54 56 197 12 24 577 Cuts at: 1790 1938 Size: 148 Fragments arranged by size: 620 577 250 197 148 56 54 24 12 ApaI G_GGCC'C Cuts at: 0 77 1572 1938 Size: 77 1495 366 Fragments arranged by size: 1495 366 77 ApaLI G'TGCA_C Cuts at: 0 1510 1938 Size: 1510 428 ApoI r'AATT_y Cuts at: 0 567 1938 Size: 567 1371 AvaI C'yCGr_G Cuts at: 0 76 145 896 1565 1938 Size: 76 69 751 669 373 Fragments arranged by size: 751 669 373 76 69 BanI G'GyrC_C Cuts at: 0 149 664 1668 1938 Size: 149 515 1004 270 Fragments arranged by size: 1004 515 270 149 BanII G_rGCy'C Cuts at: 0 77 422 772 1572 1799 1938 Size: 77 345 350 800 227 139 Fragments arranged by size: 800 350 345 227 139 77 BbeI G_GCGC'C Cuts at: 0 153 1938 Size: 153 1785 BbsI GAAGACnn'nnnn_ Cuts at: 0 658 1223 1938 Size: 658 565 715 Fragments arranged by size: 715 658 565 BbvCI CC'TCA_GC Cuts at: 0 1580 1938 Size: 1580 358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 78 112 1938 Size: 78 34 1826 Fragments arranged by size: 1826 78 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1600 1938 Size: 1600 338 BglI GCCn_nnn'nGGC Cuts at: 0 861 1055 1363 1492 1938 Size: 861 194 308 129 446 Fragments arranged by size: 861 446 308 194 129 BglII A'GATC_T Cuts at: 0 1641 1938 Size: 1641 297 Bme1580I G_kGCm'C Cuts at: 0 77 1514 1565 1572 1938 Size: 77 1437 51 7 366 Fragments arranged by size: 1437 366 77 51 7 BmrI ACTGGGnnnn_n' Cuts at: 0 745 1643 1938 Size: 745 898 295 Fragments arranged by size: 898 745 295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 668 1151 1862 1938 Size: 133 535 483 711 76 Fragments arranged by size: 711 535 483 133 76 Bpu10I CC'TnA_GC Cuts at: 0 107 1580 1938 Size: 107 1473 358 Fragments arranged by size: 1473 358 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 541 1938 Size: 502 39 1397 Fragments arranged by size: 1397 502 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 338 893 1107 1938 Size: 338 555 214 831 Fragments arranged by size: 831 555 338 214 BsaHI Gr'CG_yC Cuts at: 0 136 150 1938 Size: 136 14 1788 Fragments arranged by size: 1788 136 14 BsaWI w'CCGG_w Cuts at: 0 636 1105 1938 Size: 636 469 833 Fragments arranged by size: 833 636 469 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1591 1621 1938 Size: 1591 30 317 Fragments arranged by size: 1591 317 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1708 1938 Size: 1708 230 BseYI C'CCAG_C Cuts at: 0 1014 1182 1338 1367 1584 1790 1866 1932 Size: 1014 168 156 29 217 206 76 66 Cuts at: 1932 1938 Size: 6 Fragments arranged by size: 1014 217 206 168 156 76 66 29 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1938 Size: 322 1616 BsiEI CG_ry'CG Cuts at: 0 50 71 177 963 1938 Size: 50 21 106 786 975 Fragments arranged by size: 975 786 106 50 21 BsiHKAI G_wGCw'C Cuts at: 0 422 1311 1463 1514 1938 Size: 422 889 152 51 424 Fragments arranged by size: 889 424 422 152 51 BsmBI CGTCTCn'nnnn_ Cuts at: 0 775 793 1938 Size: 775 18 1145 Fragments arranged by size: 1145 775 18 Bsp1286I G_dGCh'C Cuts at: 0 77 422 772 1311 1463 1514 1565 1572 Size: 77 345 350 539 152 51 51 7 Cuts at: 1572 1799 1938 Size: 227 139 Fragments arranged by size: 539 350 345 227 152 139 77 51 51 7 BspEI T'CCGG_A Cuts at: 0 636 1938 Size: 636 1302 BspHI T'CATG_A Cuts at: 0 1628 1938 Size: 1628 310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 356 503 810 1680 1892 1938 Size: 356 147 307 870 212 46 Fragments arranged by size: 870 356 307 212 147 46 BsrBI CCG'CTC Cuts at: 0 61 1118 1938 Size: 61 1057 820 Fragments arranged by size: 1057 820 61 BsrFI r'CCGG_y Cuts at: 0 13 1938 Size: 13 1925 BsrGI T'GTAC_A Cuts at: 0 749 1938 Size: 749 1189 BstXI CCAn_nnnn'nTGG Cuts at: 0 678 1290 1938 Size: 678 612 648 Fragments arranged by size: 678 648 612 BstYI r'GATC_y Cuts at: 0 317 503 920 1641 1938 Size: 317 186 417 721 297 Fragments arranged by size: 721 417 317 297 186 Bsu36I CC'TnA_GG Cuts at: 0 716 1938 Size: 716 1222 BtgI C'CryG_G Cuts at: 0 708 1230 1550 1938 Size: 708 522 320 388 Fragments arranged by size: 708 522 388 320 BtsI GCAGTG_nn' Cuts at: 0 850 1254 1699 1938 Size: 850 404 445 239 Fragments arranged by size: 850 445 404 239 DraIII CAC_nnn'GTG Cuts at: 0 366 1231 1938 Size: 366 865 707 Fragments arranged by size: 865 707 366 EaeI y'GGCC_r Cuts at: 0 47 68 122 174 872 1140 1280 1493 Size: 47 21 54 52 698 268 140 213 Cuts at: 1493 1938 Size: 445 Fragments arranged by size: 698 445 268 213 140 54 52 47 21 EagI C'GGCC_G Cuts at: 0 47 68 174 1938 Size: 47 21 106 1764 Fragments arranged by size: 1764 106 47 21 EarI CTCTTCn'nnn_ Cuts at: 0 1063 1468 1652 1938 Size: 1063 405 184 286 Fragments arranged by size: 1063 405 286 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 736 1667 1938 Size: 736 931 271 Fragments arranged by size: 931 736 271 EcoICRI GAG'CTC Cuts at: 0 420 1938 Size: 420 1518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 616 668 814 1151 1235 1862 1938 Size: 133 483 52 146 337 84 627 76 Fragments arranged by size: 627 483 337 146 133 84 76 52 EcoNI CCTnn'n_nnAGG Cuts at: 0 1630 1938 Size: 1630 308 EcoO109I rG'GnC_Cy Cuts at: 0 552 1569 1938 Size: 552 1017 369 Fragments arranged by size: 1017 552 369 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 231 1068 1440 1938 Size: 231 837 372 498 Fragments arranged by size: 837 498 372 231 FspI TGC'GCA Cuts at: 0 1129 1938 Size: 1129 809 FspAI rTGC'GCAy Cuts at: 0 1129 1938 Size: 1129 809 HaeII r_GCGC'y Cuts at: 0 153 260 1938 Size: 153 107 1678 Fragments arranged by size: 1678 153 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 715 747 892 924 956 988 1062 1087 Size: 715 32 145 32 32 32 74 25 Cuts at: 1087 1094 1119 1938 Size: 7 25 819 Fragments arranged by size: 819 715 145 74 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 0 1404 1938 Size: 1404 534 KasI G'GCGC_C Cuts at: 0 149 1938 Size: 149 1789 KpnI G_GTAC'C Cuts at: 0 1672 1938 Size: 1672 266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1938 Size: 452 1486 MscI TGG'CCA Cuts at: 0 1142 1282 1938 Size: 1142 140 656 Fragments arranged by size: 1142 656 140 MslI CAynn'nnrTG Cuts at: 0 1148 1288 1480 1938 Size: 1148 140 192 458 Fragments arranged by size: 1148 458 192 140 MspA1I CmG'CkG Cuts at: 0 173 224 809 980 1342 1584 1866 1938 Size: 173 51 585 171 362 242 282 72 Fragments arranged by size: 585 362 282 242 173 171 72 51 NarI GG'CG_CC Cuts at: 0 150 1938 Size: 150 1788 NdeI CA'TA_TG Cuts at: 0 1481 1938 Size: 1481 457 NotI GC'GGCC_GC Cuts at: 0 47 174 1938 Size: 47 127 1764 Fragments arranged by size: 1764 127 47 PfoI T'CCnGG_A Cuts at: 0 1919 1938 Size: 1919 19 PpuMI rG'GwC_Cy Cuts at: 0 552 1938 Size: 552 1386 PspOMI G'GGCC_C Cuts at: 0 73 1568 1938 Size: 73 1495 370 Fragments arranged by size: 1495 370 73 PstI C_TGCA'G Cuts at: 0 353 427 1577 1694 1938 Size: 353 74 1150 117 244 Fragments arranged by size: 1150 353 244 117 74 PvuI CG_AT'CG Cuts at: 0 963 1938 Size: 963 975 PvuII CAG'CTG Cuts at: 0 980 1584 1866 1938 Size: 980 604 282 72 Fragments arranged by size: 980 604 282 72 SacI G_AGCT'C Cuts at: 0 422 1938 Size: 422 1516 SapI GCTCTTCn'nnn_ Cuts at: 0 1468 1938 Size: 1468 470 ScaI AGT'ACT Cuts at: 0 523 1938 Size: 523 1415 SfcI C'TryA_G Cuts at: 0 349 423 1573 1690 1938 Size: 349 74 1150 117 248 Fragments arranged by size: 1150 349 248 117 74 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1363 1492 1938 Size: 1363 129 446 Fragments arranged by size: 1363 446 129 SfoI GGC'GCC Cuts at: 0 151 1938 Size: 151 1787 SmaI CCC'GGG Cuts at: 0 78 147 1567 1938 Size: 78 69 1420 371 Fragments arranged by size: 1420 371 78 69 SmlI C'TyrA_G Cuts at: 0 517 556 1938 Size: 517 39 1382 Fragments arranged by size: 1382 517 39 SrfI GCCC'GGGC Cuts at: 0 78 1938 Size: 78 1860 StyI C'CwwG_G Cuts at: 0 944 1938 Size: 944 994 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1543 1938 Size: 1543 395 TatI w'GTAC_w Cuts at: 0 521 749 1837 1938 Size: 521 228 1088 101 Fragments arranged by size: 1088 521 228 101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1449 1938 Size: 1449 489 XmaI C'CCGG_G Cuts at: 0 76 145 1565 1938 Size: 76 69 1420 373 Fragments arranged by size: 1420 373 76 69 ZraI GAC'GTC Cuts at: 0 137 1938 Size: 137 1801 Enzymes that do cut: AatII Acc65I AcuI AleI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NdeI NotI PfoI PpuMI PspOMI PstI PvuI PvuII SacI SapI ScaI SfcI SfiI SfoI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FseI HindIII HpaI MfeI MluI NaeI NcoI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI