RASD1 ORF clone pFN21AB5186

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Halotag ORF Clone pFN21AB5186

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Clone info.

Species

human

Clone Name

pFN21AB5186 Sequence (with vector) Restriction Map

Product ID

FHC06597

Flexi Clone PID

FXC06597

ORF Sequence

DNA sequence (843 bp)
Protein sequence (281 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528221]

Gene

KIBB5186

Symbol

RASD1

Alias : AGS1, DEXRAS1, MGC:26290

Description

RAS, dexamethasone-induced 1, transcript variant 1

Original Clone ID

bm01353

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 843 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 281 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11185.1 3.1e-106 100.0 RASD1 CCDS13916.1 4.2e-55 62.0 RASD2 CCDS58519.1 6.8e-32 100.0 RASD1 CCDS12092.1 3.1e-16 41.2 DIRAS1 CCDS6687.1 9.3e-16 36.2 DIRAS2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 25 46 PR00449 Ras GTPase IPR001806 48 64 PR00449 Ras GTPase IPR001806 65 87 PR00449 Ras GTPase IPR001806 136 149 PR00449 Ras GTPase IPR001806 173 195 PR00449 Ras GTPase HMMPfam IPR013753 26 192 PF00071 Ras HMMSmart IPR003577 22 198 SM00173 Ras small GTPase IPR003579 25 198 SM00175 Ras small GTPase IPR003578 27 196 SM00174 Small GTPase IPR002041 30 243 SM00176 Ran GTPase HMMTigr IPR005225 23 190 TIGR00231 Small GTP-binding protein domain ProfileScan IPR020849 15 281 PS51421 Ras small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057168 281 605550 100.0 100.0 XP_016884190 278 612842 62.1 98.2 XP_016884191 266 612842 62.0 97.9 XP_005261499 266 612842 62.0 97.9 NP_055125 266 612842 62.0 97.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5186 Download

>KIBB5186  843 bp
ATGAAACTGGCCGCGATGATCAAGAAGATGTGCCCGAGCGACTCGGAGCTGAGTATCCCG
GCCAAGAACTGCTATCGCATGGTCATCCTCGGCTCGTCCAAGGTGGGCAAGACGGCCATC
GTGTCGCGCTTCCTCACCGGCCGCTTCGAGGACGCCTACACGCCTACCATCGAGGACTTC
CACCGCAAGTTCTACTCCATCCGCGGCGAGGTCTACCAGCTCGACATCCTCGACACGTCC
GGCAACCACCCGTTCCCCGCCATGCGGCGCCTCTCCATCCTCACAGGAGACGTTTTCATC
CTGGTGTTCAGTCTGGACAACCGCGACTCCTTCGAGGAGGTGCAGCGGCTCAGGCAGCAG
ATCCTCGACACCAAGTCTTGCCTCAAGAACAAAACCAAGGAGAACGTGGACGTGCCCCTG
GTCATCTGCGGCAACAAGGGTGACCGCGACTTCTACCGCGAGGTGGACCAGCGCGAGATC
GAGCAGCTGGTGGGCGACGACCCCCAGCGCTGCGCCTACTTCGAGATCTCGGCCAAGAAG
AACAGCAGCCTGGACCAGATGTTCCGCGCGCTCTTCGCCATGGCCAAGCTGCCCAGCGAG
ATGAGCCCAGACCTGCACCGCAAGGTCTCGGTGCAGTACTGCGACGTGCTGCACAAGAAG
GCGCTGCGGAACAAGAAGCTGCTGCGGGCCGGCAGCGGCGGCGGCGGCGGCGACCCGGGC
GACGCCTTTGGCATCGTGGCACCCTTCGCGCGCCGGCCCAGCGTACACAGCGACCTCATG
TACATCCGCGAGAAGGCCAGCGCCGGCAGCCAGGCCAAGGACAAGGAGCGCTGCGTCATC
AGC

ORF Protein

Cloned ORF protein sequence for pFN21AB5186 Download

>KIBB5186  281 aa
MKLAAMIKKMCPSDSELSIPAKNCYRMVILGSSKVGKTAIVSRFLTGRFEDAYTPTIEDF
HRKFYSIRGEVYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRDSFEEVQRLRQQ
ILDTKSCLKNKTKENVDVPLVICGNKGDRDFYREVDQREIEQLVGDDPQRCAYFEISAKK
NSSLDQMFRALFAMAKLPSEMSPDLHRKVSVQYCDVLHKKALRNKKLLRAGSGGGGGDPG
DAFGIVAPFARRPSVHSDLMYIREKASAGSQAKDKERCVIS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5186 Download

>pFN21AB5186  5548 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAACTGGCCGCGATGATCAAGAAGATGTGCCC
GAGCGACTCGGAGCTGAGTATCCCGGCCAAGAACTGCTATCGCATGGTCATCCTCGGCTC
GTCCAAGGTGGGCAAGACGGCCATCGTGTCGCGCTTCCTCACCGGCCGCTTCGAGGACGC
CTACACGCCTACCATCGAGGACTTCCACCGCAAGTTCTACTCCATCCGCGGCGAGGTCTA
CCAGCTCGACATCCTCGACACGTCCGGCAACCACCCGTTCCCCGCCATGCGGCGCCTCTC
CATCCTCACAGGAGACGTTTTCATCCTGGTGTTCAGTCTGGACAACCGCGACTCCTTCGA
GGAGGTGCAGCGGCTCAGGCAGCAGATCCTCGACACCAAGTCTTGCCTCAAGAACAAAAC
CAAGGAGAACGTGGACGTGCCCCTGGTCATCTGCGGCAACAAGGGTGACCGCGACTTCTA
CCGCGAGGTGGACCAGCGCGAGATCGAGCAGCTGGTGGGCGACGACCCCCAGCGCTGCGC
CTACTTCGAGATCTCGGCCAAGAAGAACAGCAGCCTGGACCAGATGTTCCGCGCGCTCTT
CGCCATGGCCAAGCTGCCCAGCGAGATGAGCCCAGACCTGCACCGCAAGGTCTCGGTGCA
GTACTGCGACGTGCTGCACAAGAAGGCGCTGCGGAACAAGAAGCTGCTGCGGGCCGGCAG
CGGCGGCGGCGGCGGCGACCCGGGCGACGCCTTTGGCATCGTGGCACCCTTCGCGCGCCG
GCCCAGCGTACACAGCGACCTCATGTACATCCGCGAGAAGGCCAGCGCCGGCAGCCAGGC
CAAGGACAAGGAGCGCTGCGTCATCAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT