Restriction map for
pFN21AB5186
(Linear) (Six-base) MAPSORT of: KIBB5186.seq Check: 142 from: 1 to: 843
KIBB5186.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 212 843
Size: 212 631
AfeI AGC'GCT
Cuts at: 0 508 829 843
Size: 508 321 14
Fragments arranged by size:
508 321 14
AflIII A'CryG_T
Cuts at: 0 233 843
Size: 233 610
AhdI GACnn_n'nnGTC
Cuts at: 0 372 843
Size: 372 471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 843
Size: 544 32 267
Fragments arranged by size:
544 267 32
AvaI C'yCGr_G
Cuts at: 0 33 714 843
Size: 33 681 129
Fragments arranged by size:
681 129 33
BanI G'GyrC_C
Cuts at: 0 266 738 843
Size: 266 472 105
Fragments arranged by size:
472 266 105
BanII G_rGCy'C
Cuts at: 0 607 843
Size: 607 236
BbeI G_GCGC'C
Cuts at: 0 270 843
Size: 270 573
BciVI GTATCCnnnnn_n'
Cuts at: 0 64 843
Size: 64 779
BclI T'GATC_A
Cuts at: 0 17 843
Size: 17 826
BglII A'GATC_T
Cuts at: 0 524 843
Size: 524 319
Bme1580I G_kGCm'C
Cuts at: 0 34 416 843
Size: 34 382 427
Fragments arranged by size:
427 382 34
BmgBI CAC'GTC
Cuts at: 0 236 411 645 843
Size: 236 175 234 198
Fragments arranged by size:
236 234 198 175
Bpu10I CC'TnA_GC
Cuts at: 0 349 843
Size: 349 494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 843
Size: 367 476
BsaI GGTCTCn'nnnn_
Cuts at: 0 630 843
Size: 630 213
BsaHI Gr'CG_yC
Cuts at: 0 152 267 722 843
Size: 152 115 455 121
Fragments arranged by size:
455 152 121 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 843
Size: 310 30 503
Fragments arranged by size:
503 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 843
Size: 349 494
BseYI C'CCAG_C
Cuts at: 0 503 592 757 843
Size: 503 89 165 86
Fragments arranged by size:
503 165 89 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 598 634 652 843
Size: 361 237 36 18 191
Fragments arranged by size:
361 237 191 36 18
BsiEI CG_ry'CG
Cuts at: 0 141 843
Size: 141 702
BsmBI CGTCTCn'nnnn_
Cuts at: 0 281 843
Size: 281 562
Bsp1286I G_dGCh'C
Cuts at: 0 34 416 607 843
Size: 34 382 191 236
Fragments arranged by size:
382 236 191 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 843
Size: 620 223
BsrFI r'CCGG_y
Cuts at: 0 136 688 752 802 843
Size: 136 552 64 50 41
Fragments arranged by size:
552 136 64 50 41
BsrGI T'GTAC_A
Cuts at: 0 779 843
Size: 779 64
BssHII G'CGCG_C
Cuts at: 0 566 748 843
Size: 566 182 95
Fragments arranged by size:
566 182 95
BstEII G'GTnAC_C
Cuts at: 0 439 843
Size: 439 404
BstYI r'GATC_y
Cuts at: 0 359 524 843
Size: 359 165 319
Fragments arranged by size:
359 319 165
BtgI C'CryG_G
Cuts at: 0 201 578 843
Size: 201 377 265
Fragments arranged by size:
377 265 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 843
Size: 28 815
EaeI y'GGCC_r
Cuts at: 0 8 59 113 138 530 581 843
Size: 8 51 54 25 392 51 262
Fragments arranged by size:
392 262 54 51 51 25 8
EagI C'GGCC_G
Cuts at: 0 138 843
Size: 138 705
EarI CTCTTCn'nnn_
Cuts at: 0 577 843
Size: 577 266
HaeII r_GCGC'y
Cuts at: 0 270 510 664 803 831 843
Size: 270 240 154 139 28 12
Fragments arranged by size:
270 240 154 139 28 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 843
Size: 358 32 453
Fragments arranged by size:
453 358 32
KasI G'GCGC_C
Cuts at: 0 266 843
Size: 266 577
MscI TGG'CCA
Cuts at: 0 583 843
Size: 583 260
MslI CAynn'nnrTG
Cuts at: 0 301 843
Size: 301 542
MspA1I CmG'CkG
Cuts at: 0 203 345 486 695 843
Size: 203 142 141 209 148
Fragments arranged by size:
209 203 148 142 141
NaeI GCC'GGC
Cuts at: 0 690 754 804 843
Size: 690 64 50 39
Fragments arranged by size:
690 64 50 39
NarI GG'CG_CC
Cuts at: 0 267 843
Size: 267 576
NcoI C'CATG_G
Cuts at: 0 578 843
Size: 578 265
NgoMIV G'CCGG_C
Cuts at: 0 688 752 802 843
Size: 688 64 50 41
Fragments arranged by size:
688 64 50 41
PvuII CAG'CTG
Cuts at: 0 486 843
Size: 486 357
SacII CC_GC'GG
Cuts at: 0 204 843
Size: 204 639
SapI GCTCTTCn'nnn_
Cuts at: 0 577 843
Size: 577 266
ScaI AGT'ACT
Cuts at: 0 637 843
Size: 637 206
SfoI GGC'GCC
Cuts at: 0 268 843
Size: 268 575
SmaI CCC'GGG
Cuts at: 0 716 843
Size: 716 127
SmlI C'TyrA_G
Cuts at: 0 382 843
Size: 382 461
StyI C'CwwG_G
Cuts at: 0 98 395 578 815 843
Size: 98 297 183 237 28
Fragments arranged by size:
297 237 183 98 28
TatI w'GTAC_w
Cuts at: 0 635 779 843
Size: 635 144 64
Fragments arranged by size:
635 144 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 843
Size: 402 441
XmaI C'CCGG_G
Cuts at: 0 714 843
Size: 714 129
Enzymes that do cut:
AccI AfeI AflIII AhdI AloI AvaI BanI BanII
BbeI BciVI BclI BglII Bme1580I BmgBI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrFI BsrGI BssHII BstEII BstYI BtgI
BtgZI EaeI EagI EarI HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PvuII SacII
SapI ScaI SfoI SmaI SmlI StyI TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BfrBI BglI
BlpI BmrI BmtI BplI BpmI BsaAI BsaBI BsaWI
BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SbfI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI