Restriction map for
pFN21AB5186
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsmFI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 211 GTCTAC GT^[AC][GT]AC
AflIII 233 ACACGT A^C[GA][CT]GT
AhdI 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 360 GATCC GGATC(4/5)
AspEI 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 581 TGGCCA TGG^CCA
BanII 603 GAGCCC G[GA]GC[CT]^C
BbeI 266 GGCGCC GGCGC^C
BciVI 53 GTATCC GTATCC(6/5)
BclI 17 TGATCA T^GATCA
BglII 524 AGATCT A^GATCT
BsaI 624 GGTCTC GGTCTC(1/5)
BseRI 334 GAGGAG GAGGAG(10/8)
BsiEI 138 CGGCCG CG[GA][CT]^CG
BsmBI 287 GAGACG CGTCTC(1/5)
Bsp1407I 779 TGTACA T^GTACA
BspMI 611 ACCTGC ACCTGC(4/8)
BsrGI 779 TGTACA T^GTACA
BsrI 6 ACTGG ACTGG(1/-1)
BstEII 439 GGTGACC G^GT[ACGT]ACC
BstPI 439 GGTGACC G^GT[ACGT]ACC
EagI 138 CGGCCG C^GGCCG
Eam1105I 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 571 CTCTTC CTCTTC(1/4)
EclXI 138 CGGCCG C^GGCCG
Eco52I 138 CGGCCG C^GGCCG
EcoO65I 439 GGTGACC G^GT[ACGT]ACC
FbaI 17 TGATCA T^GATCA
KasI 266 GGCGCC G^GCGCC
Ksp632I 571 CTCTTC CTCTTC(1/4)
KspI 201 CCGCGG CCGC^GG
MaeIII 440 GTGAC ^GT[ACGT]AC
MluNI 581 TGGCCA TGG^CCA
MscI 581 TGGCCA TGG^CCA
MslI 297 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 266 GGCGCC GG^CGCC
NcoI 578 CCATGG C^CATGG
PvuII 484 CAGCTG CAG^CTG
SacII 201 CCGCGG CCGC^GG
SapI 570 GCTCTTC GCTCTTC(1/4)
ScaI 635 AGTACT AGT^ACT
SfaNI 731 GCATC GCATC(5/9)
SfoI 266 GGCGCC GGC^GCC
SmaI 714 CCCGGG CCC^GGG
SmlI 382 CTCAAG C^T[CT][GA]AG
SspBI 779 TGTACA T^GTACA
Tsp45I 440 GTGAC ^GT[GC]AC
XcmI 395 CCAAGGAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 714 CCCGGG C^CCGGG
XmaI 714 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 506 AGCGCT AGC^GCT
827 AGCGCT
AvaI 33 CCCGAG C^[CT]CG[GA]G
714 CCCGGG
AvaII 465 GGACC G^G[AT]CC
552 GGACC
BanI 266 GGCGCC G^G[CT][GA]CC
738 GGCACC
BsmAI 287 GAGAC GTCTC(1/5)
625 GTCTC
BsoBI 33 CCCGAG C^[CT]CG[GA]G
714 CCCGGG
BssHII 566 GCGCGC G^CGCGC
748 GCGCGC
BstYI 359 AGATCC [GA]^GATC[CT]
524 AGATCT
DdeI 49 CTGAG C^T[ACGT]AG
349 CTCAG
DsaI 201 CCGCGG C^C[GA][CT]GG
578 CCATGG
Eco47III 506 AGCGCT AGC^GCT
827 AGCGCT
FauI 256 CCCGC CCCGC(4/6)
684 GCGGG
HinfI 40 GACTC G^A[ACGT]TC
325 GACTC
HphI 439 GGTGA GGTGA(8/7)
134 TCACC
MflI 359 AGATCC [GA]^GATC[CT]
524 AGATCT
NlaIV 266 GGCGCC GG[ACGT]^[ACGT]CC
738 GGCACC
PleI 40 GACTC GAGTC(4/5)
325 GACTC
XhoII 359 AGATCC [GA]^GATC[CT]
524 AGATCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BbvI BcnI BmyI BsaHI BsaJI
BsgI BsiYI BslI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII RsaI Sau3AI Sau96I
ScrFI StyI TaqI TseI TthHB8I