CALB1 ORF clone pFN21AB4989

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Halotag ORF Clone pFN21AB4989

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Clone info.

Species

human

Clone Name

pFN21AB4989 Sequence (with vector) Restriction Map

Product ID

FHC11559

Flexi Clone PID

FXC11559

Original Clone PID

ORK11559

ORF Sequence

DNA sequence (783 bp)
Protein sequence (261 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529128]

Gene

KIBB4989

Symbol

CALB1

Alias : CALB, D-28K

Description

calbindin 1, 28kDa

Original Clone ID

bm00929

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 783 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 261 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6251.1 2.4e-90 100.0 CALB1 CCDS10899.1 3.4e-52 58.7 CALB2 CCDS4561.1 9e-26 37.6 SCGN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018248 102 130 PF00036 EF-hand IPR018248 190 217 PF00036 EF-hand HMMSmart IPR002048 15 43 SM00054 Calcium-binding EF-hand IPR002048 102 130 SM00054 Calcium-binding EF-hand IPR002048 146 174 SM00054 Calcium-binding EF-hand IPR002048 190 218 SM00054 Calcium-binding EF-hand ProfileScan IPR018249 11 46 PS50222 EF-HAND 2 IPR018249 53 88 PS50222 EF-HAND 2 IPR018249 98 133 PS50222 EF-HAND 2 IPR018249 142 177 PS50222 EF-HAND 2 IPR018249 186 221 PS50222 EF-HAND 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004920 261 114050 100.0 100.0 NP_001731 271 114051 58.7 97.3 NP_008929 276 609202 37.6 95.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4989 Download

>KIBB4989  783 bp
ATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGATCTGG
CTCCATTTCGACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATC
CAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAAAACT
TTTGTGGATCAGTATGGGCAAAGAGATGATGGAAAAATAGGAATTGTAGAGTTGGCTCAC
GTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGATGCCAGCAGCTGAAGTCCTGT
GAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTTCATAGAAACT
GAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGATGAC
ACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGATGGG
AAGCTGGAATTAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAAA
TTCCAGGGAATCAAAATGTGTGGGAAAGAGTTCAATAAGGCTTTTGAGCTGTATGATCAG
GACGGCAATGGATACATAGATGAAAATGAACTGGATGCTTTACTGAAGGATCTGTGCGAG
AAGAATAAACAGGATCTGGATATTAATAATATTACAACATACAAGAAGAACATAATGGCT
TTGTCGGATGGAGGGAAGCTGTACCGAACGGATCTTGCTCTTATTCTCTGTGCTGGGGAT
AAC

ORF Protein

Cloned ORF protein sequence for pFN21AB4989 Download

>KIBB4989  261 aa
MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKT
FVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIET
EELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLK
FQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMA
LSDGGKLYRTDLALILCAGDN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4989 Download

>pFN21AB4989  5488 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGAATCCCACCTGCAGTCATCCCTCATCAC
AGCCTCACAGTTTTTCGAGATCTGGCTCCATTTCGACGCTGACGGAAGTGGTTACCTGGA
AGGAAAGGAGCTGCAGAACTTGATCCAGGAGCTCCAGCAGGCGCGAAAGAAGGCTGGATT
GGAGTTATCACCTGAAATGAAAACTTTTGTGGATCAGTATGGGCAAAGAGATGATGGAAA
AATAGGAATTGTAGAGTTGGCTCACGTATTACCCACAGAAGAGAATTTCCTGCTGCTCTT
CCGATGCCAGCAGCTGAAGTCCTGTGAGGAATTCATGAAGACATGGAGAAAATATGATAC
TGACCACAGTGGCTTCATAGAAACTGAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGA
AAAAGCAAACAAGACTGTTGATGACACAAAATTAGCCGAGTATACAGACCTAATGCTGAA
ACTATTTGATTCAAATAATGATGGGAAGCTGGAATTAACTGAGATGGCCAGGTTACTACC
AGTGCAGGAGAATTTTCTTCTTAAATTCCAGGGAATCAAAATGTGTGGGAAAGAGTTCAA
TAAGGCTTTTGAGCTGTATGATCAGGACGGCAATGGATACATAGATGAAAATGAACTGGA
TGCTTTACTGAAGGATCTGTGCGAGAAGAATAAACAGGATCTGGATATTAATAATATTAC
AACATACAAGAAGAACATAATGGCTTTGTCGGATGGAGGGAAGCTGTACCGAACGGATCT
TGCTCTTATTCTCTGTGCTGGGGATAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT