Restriction map for
pFN21AB4989
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BcnI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MslI MspI MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 435 GTATAC GT^[AC][GT]AC
AccII 137 CGCG CG^CG
AfaI 741 GTAC GT^AC
AflII 367 CTTAAG C^TTAAG
AseI 682 ATTAAT AT^TAAT
AsnI 682 ATTAAT AT^TAAT
Asp700I 564 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 124 GAGCTC G[AT]GC[AT]^C
AvaII 384 GGACC G^G[AT]CC
BalI 500 TGGCCA TGG^CCA
BanII 124 GAGCTC G[GA]GC[CT]^C
BbsI 312 GAAGAC GAAGAC(2/6)
BciVI 610 GGATAC GTATCC(6/5)
BclI 594 TGATCA T^GATCA
BfaI 391 CTAG C^TAG
BfrI 367 CTTAAG C^TTAAG
BglII 53 AGATCT A^GATCT
BmyI 124 GAGCTC G[AGT]GC[ACT]^C
BpmI 127 CTCCAG CTGGAG(16/14)
BpuAI 312 GAAGAC GAAGAC(2/6)
BsaAI 238 CACGTA [CT]AC^GT[GA]
BsaJI 543 CCAGGG C^C[ACGT][ACGT]GG
BseRI 361 GAGGAG GAGGAG(10/8)
BsgI 517 GTGCAG GTGCAG(16/14)
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
Bsp1286I 124 GAGCTC G[AGT]GC[ACT]^C
BspHI 308 TCATGA T^CATGA
BsrDI 605 GCAATG GCAATG(2/0)
Bst1107I 435 GTATAC GTA^TAC
BstEII 85 GGTTACC G^GT[ACGT]ACC
BstPI 85 GGTTACC G^GT[ACGT]ACC
BstUI 137 CGCG CG^CG
BstZ17I 435 GTATAC GTA^TAC
CfoI 136 GCGC GCG^C
Cfr13I 384 GGACC G^G[ACGT]CC
DraII 383 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 500 TGGCCA [CT]^GGCC[GA]
EcoO109I 383 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 85 GGTTACC G^GT[ACGT]ACC
EcoRI 304 GAATTC G^AATTC
FbaI 594 TGATCA T^GATCA
HaeIII 501 GGCC GG^CC
HgaI 70 GACGC GACGC(5/10)
HhaI 136 GCGC GCG^C
HinP1I 136 GCGC G^CGC
HphI 163 TCACC GGTGA(8/7)
MaeI 391 CTAG C^TAG
MaeII 239 ACGT A^CGT
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
MspA1I 286 CAGCTG C[AC]G^C[GT]G
MvnI 137 CGCG CG^CG
NlaIV 59 GGCTCC GG[ACGT]^[ACGT]CC
PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 682 ATTAAT AT^TAAT
PvuII 286 CAGCTG CAG^CTG
RcaI 308 TCATGA T^CATGA
RsaI 741 GTAC GT^AC
SacI 124 GAGCTC GAGCT^C
SapI 270 GCTCTTC GCTCTTC(1/4)
Sau96I 384 GGACC G^G[ACGT]CC
SmlI 367 CTTAAG C^T[CT][GA]AG
SspI 688 AATATT AAT^ATT
XmnI 564 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 283 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
670 CAGGATCTG
BspMI 14 ACCTGC ACCTGC(4/8)
386 ACCTGC
BsrI 630 ACTGG ACTGG(1/-1)
514 CCAGT
Cac8I 132 GCAGGC GC[ACGT]^[ACGT]GC
281 GCCAGC
DdeI 359 CTGAG C^T[ACGT]AG
494 CTGAG
EarI 271 CTCTTC CTCTTC(1/4)
253 GAAGAG
Ksp632I 271 CTCTTC CTCTTC(1/4)
253 GAAGAG
MaeIII 86 GTTAC ^GT[ACGT]AC
507 GTTAC
NlaIII 309 CATG CATG^
317 CATG
PstI 16 CTGCAG CTGCA^G
106 CTGCAG
SfaNI 278 GATGC GCATC(5/9)
634 GATGC
SfcI 16 CTGCAG C^T[GA][CT]AG
106 CTGCAG
TaqI 50 TCGA T^CGA
68 TCGA
TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^
515 CAGTG
TthHB8I 50 TCGA T^CGA
68 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BssKI BstF5I BstNI BstYI DpnII
EcoRII Fnu4HI FokI HinfI ItaI
MboI MboII MflI MnlI MseI
MvaI NdeII Sau3AI ScrFI TfiI
Tru9I TseI Tsp509I XhoII