PRIM1 ORF clone pFN21AB0958

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Halotag ORF Clone pFN21AB0958

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Clone info.

Species

human

Clone Name

pFN21AB0958 Sequence (with vector) Restriction Map

Product ID

FHC08234

Flexi Clone PID

FXC08234

ORF Sequence

DNA sequence (1260 bp)
Protein sequence (420 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528338]

Gene

KIBB0958

Symbol

PRIM1

Alias : p49

Description

primase, DNA, polypeptide 1 (49kDa)

Original Clone ID

cs02058

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1260 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 420 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44926.1 9.1e-183 100.0 PRIM1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002755 108 335 PF01896 DNA primase HMMTigr IPR014052 14 349 TIGR00335 DNA primase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000937 420 176635 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB0958 Download

>KIBB0958  1260 bp
ATGGAGACGTTTGACCCCACCGAGCTGCCCGAGCTGCTTAAACTTTATTACCGGAGGCTC
TTTCCCTACTCTCAGTACTATCGCTGGCTCAACTACGGTGGAGTGATAAAGAATTACTTT
CAACACCGTGAATTTTCATTCACATTGAAAGATGATATTTACATTCGCTACCAATCCTTC
AACAACCAGAGTGATCTGGAAAAGGAGATGCAGAAAATGAATCCATACAAGATTGATATA
GGCGCAGTATATTCTCACAGACCCAATCAACACAATACAGTGAAGCTGGGAGCTTTCCAG
GCTCAGGAAAAAGAACTGGTATTTGACATTGACATGACAGACTATGACGATGTGAGGAGA
TGTTGTAGTTCTGCAGACATATGTCCTAAGTGCTGGACCCTCATGACAATGGCCATACGC
ATCATTGACAGAGCATTGAAGGAGGACTTTGGATTTAAGCATCGTCTCTGGGTATATTCT
GGAAGGAGAGGTGTTCATTGTTGGGTCTGTGATGAATCAGTTAGAAAACTGTCTTCTGCA
GTACGTTCTGGGATAGTTGAGTATTTGAGCCTTGTAAAGGGTGGTCAAGACGTTAAAAAG
AAAGTTCACCTAAGTGAAAAAATTCACCCTTTTATCAGAAAATCTATAAACATAATAAAA
AAATACTTTGAAGAATATGCCTTGGTTAATCAAGATATTCTCGAAAATAAAGAAAGCTGG
GATAAGATTTTAGCCCTTGTTCCTGAAACAATTCATGATGAACTTCAACAAAGCTTCCAA
AAGTCTCACAATTCACTTCAGCGTTGGGAGCACTTGAAGAAAGTAGCCAGCAGATATCAG
AATAACATCAAAAATGACAAATATGGACCCTGGCTGGAGTGGGAGATTATGCTCCAGTAC
TGTTTTCCACGGCTGGATATCAATGTCAGCAAAGGAATCAATCATCTACTGAAGAGCCCT
TTTAGTGTTCATCCTAAAACAGGTCGCATATCTGTGCCTATTGATTTGCAGAAAGTGGAC
CAGTTTGATCCATTTACTGTTCCGACCATAAGCTTCATCTGCCGTGAATTGGATGCCATT
TCCACTAATGAAGAGGAAAAAGAGGAGAATGAAGCTGAATCTGATGTCAAACATAGAACC
AGAGATTATAAGAAGACCAGTCTAGCACCTTATGTGAAAGTTTTTGAACATTTTCTTGAA
AATCTGGATAAATCCCGAAAAGGAGAACTTCTTAAGAAGAGTGATTTACAAAAAGATTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB0958 Download

>KIBB0958  420 aa
METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSF
NNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRR
CCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSA
VRSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINIIKKYFEEYALVNQDILENKESW
DKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQY
CFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAI
STNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB0958 Download

>pFN21AB0958  5965 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGACGTTTGACCCCACCGAGCTGCCCGAGCT
GCTTAAACTTTATTACCGGAGGCTCTTTCCCTACTCTCAGTACTATCGCTGGCTCAACTA
CGGTGGAGTGATAAAGAATTACTTTCAACACCGTGAATTTTCATTCACATTGAAAGATGA
TATTTACATTCGCTACCAATCCTTCAACAACCAGAGTGATCTGGAAAAGGAGATGCAGAA
AATGAATCCATACAAGATTGATATAGGCGCAGTATATTCTCACAGACCCAATCAACACAA
TACAGTGAAGCTGGGAGCTTTCCAGGCTCAGGAAAAAGAACTGGTATTTGACATTGACAT
GACAGACTATGACGATGTGAGGAGATGTTGTAGTTCTGCAGACATATGTCCTAAGTGCTG
GACCCTCATGACAATGGCCATACGCATCATTGACAGAGCATTGAAGGAGGACTTTGGATT
TAAGCATCGTCTCTGGGTATATTCTGGAAGGAGAGGTGTTCATTGTTGGGTCTGTGATGA
ATCAGTTAGAAAACTGTCTTCTGCAGTACGTTCTGGGATAGTTGAGTATTTGAGCCTTGT
AAAGGGTGGTCAAGACGTTAAAAAGAAAGTTCACCTAAGTGAAAAAATTCACCCTTTTAT
CAGAAAATCTATAAACATAATAAAAAAATACTTTGAAGAATATGCCTTGGTTAATCAAGA
TATTCTCGAAAATAAAGAAAGCTGGGATAAGATTTTAGCCCTTGTTCCTGAAACAATTCA
TGATGAACTTCAACAAAGCTTCCAAAAGTCTCACAATTCACTTCAGCGTTGGGAGCACTT
GAAGAAAGTAGCCAGCAGATATCAGAATAACATCAAAAATGACAAATATGGACCCTGGCT
GGAGTGGGAGATTATGCTCCAGTACTGTTTTCCACGGCTGGATATCAATGTCAGCAAAGG
AATCAATCATCTACTGAAGAGCCCTTTTAGTGTTCATCCTAAAACAGGTCGCATATCTGT
GCCTATTGATTTGCAGAAAGTGGACCAGTTTGATCCATTTACTGTTCCGACCATAAGCTT
CATCTGCCGTGAATTGGATGCCATTTCCACTAATGAAGAGGAAAAAGAGGAGAATGAAGC
TGAATCTGATGTCAAACATAGAACCAGAGATTATAAGAAGACCAGTCTAGCACCTTATGT
GAAAGTTTTTGAACATTTTCTTGAAAATCTGGATAAATCCCGAAAAGGAGAACTTCTTAA
GAAGAGTGATTTACAAAAAGATTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT