(Circular) (Six-base) MAPSORT of: pFN21AB0958.seq Check: 2688 from: 1 to: 5965 pFN21AB0958 5965 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 2975 4028 5412 5680 1632 Size: 1154 189 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1154 1053 268 189 AflII C'TTAA_G Cuts at: 820 1017 3236 820 Size: 197 2219 3549 Fragments arranged by size: 3549 2219 197 AflIII A'CryG_T Cuts at: 1203 4864 5841 1203 Size: 3661 977 1327 Fragments arranged by size: 3661 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 2616 1166 Size: 1450 4515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3014 1184 Size: 1830 4135 AlwNI CAG_nnn'CTG Cuts at: 3708 5280 3708 Size: 1572 4393 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 2135 2625 3274 3500 3652 5761 1493 Size: 642 490 649 226 152 2109 1697 Fragments arranged by size: 2109 1697 649 642 490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 2033 3290 1870 Size: 73 90 1257 4545 Fragments arranged by size: 4545 1257 90 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3286 4741 618 Size: 325 217 627 1499 1455 1842 Fragments arranged by size: 1842 1499 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2963 3284 1391 Size: 1572 321 4072 Fragments arranged by size: 4072 1572 321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2529 3164 928 Size: 971 630 635 3729 Fragments arranged by size: 3729 971 635 630 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 1548 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4576 136 Size: 107 122 71 631 605 2904 1525 Fragments arranged by size: 2904 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5960 5960 Size: 5965 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 4026 5182 1790 Size: 2236 1156 2573 Fragments arranged by size: 2573 2236 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3696 4654 442 Size: 416 53 83 399 24 2279 958 1753 Fragments arranged by size: 2279 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2882 2900 4625 1239 Size: 1643 18 1725 2579 Fragments arranged by size: 2579 1725 1643 18 Bpu10I CC'TnA_GC Cuts at: 2307 2307 Size: 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4090 4955 5253 4090 Size: 865 298 4802 Fragments arranged by size: 4802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3772 4154 4789 275 Size: 53 83 186 936 2239 382 635 1451 Fragments arranged by size: 2239 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 4398 4796 5070 5217 5628 2055 Size: 2343 398 274 147 411 2392 Fragments arranged by size: 2392 2343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2353 2383 1178 Size: 30 165 30 950 30 4760 Fragments arranged by size: 4760 950 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2374 3122 1186 Size: 1188 748 4029 Fragments arranged by size: 4029 1188 748 BseYI C'CCAG_C Cuts at: 2290 2721 5168 5664 2290 Size: 431 2447 496 2591 Fragments arranged by size: 2591 2447 496 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3415 4176 5204 1540 Size: 275 186 1414 761 1028 2301 Fragments arranged by size: 2301 1414 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 4026 4111 5182 2817 Size: 1209 85 1071 3600 Fragments arranged by size: 3600 1209 1071 85 BsmI GAATG_Cn' Cuts at: 3476 3569 3476 Size: 93 5872 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2474 2003 Size: 471 5494 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2817 2963 3284 4026 4111 5182 1391 Size: 399 1027 146 321 742 85 1071 2174 Fragments arranged by size: 2174 1071 1027 742 399 321 146 85 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 2406 2758 3854 5584 2406 Size: 352 1096 1730 2787 Fragments arranged by size: 2787 1730 1096 352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 844 Size: 2476 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3852 1394 Size: 429 2029 3507 Fragments arranged by size: 3507 2029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4460 4634 65 Size: 1183 3212 174 1396 Fragments arranged by size: 3212 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4609 4796 1700 Size: 110 2799 187 2869 Fragments arranged by size: 2869 2799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5965 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5965 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 3295 3662 4047 4064 5516 5602 5614 5960 Size: 1323 367 385 17 1452 86 12 346 Cuts at: 5960 1972 Size: 1977 Fragments arranged by size: 1977 1452 1323 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2912 513 Size: 552 939 908 3566 Fragments arranged by size: 3566 939 908 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 3481 4275 4295 3481 Size: 794 20 5151 Fragments arranged by size: 5151 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2083 2118 529 Size: 35 1519 35 4376 Fragments arranged by size: 4376 1519 35 35 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2415 3412 4301 Size: 54 1425 50 275 18 585 997 889 Cuts at: 4301 5930 8 Size: 1629 43 Fragments arranged by size: 1629 1425 997 889 585 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3412 1537 Size: 275 1600 4090 Fragments arranged by size: 4090 1600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2951 3090 3236 3895 1353 Size: 1598 139 146 659 3423 Fragments arranged by size: 3423 1598 659 146 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4552 4926 5072 387 Size: 1064 138 2963 374 146 1280 Fragments arranged by size: 2963 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2786 2882 2900 2975 4028 4625 5412 Size: 393 1154 96 18 75 1053 597 787 Cuts at: 5412 5680 1239 Size: 268 1524 Fragments arranged by size: 1524 1154 1053 787 597 393 268 96 75 18 EcoNI CCTnn'n_nnAGG Cuts at: 2579 2982 2579 Size: 403 5562 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 EcoRV GAT'ATC Cuts at: 2840 2923 2840 Size: 83 5882 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 2467 3248 2137 Size: 330 781 4854 Fragments arranged by size: 4854 781 330 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 5112 1678 Size: 3434 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373 Size: 32 135 32 3034 32 42 32 2626 Fragments arranged by size: 3034 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3309 3555 669 Size: 988 281 1371 246 3079 Fragments arranged by size: 3079 1371 988 281 246 HindIII A'AGCT_T Cuts at: 748 2776 3055 748 Size: 2028 279 3658 Fragments arranged by size: 3658 2028 279 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3071 5079 5263 1305 Size: 1766 2008 184 2007 Fragments arranged by size: 2008 2007 1766 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 10 Size: 54 1425 343 585 3558 Fragments arranged by size: 3558 1425 585 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2411 2616 2996 3923 4282 4441 Size: 648 1170 75 205 380 927 359 159 Cuts at: 4441 518 Size: 2042 Fragments arranged by size: 2042 1170 927 648 380 359 205 159 75 MspA1I CmG'CkG Cuts at: 1606 3381 4058 5206 5451 1606 Size: 1775 677 1148 245 2120 Fragments arranged by size: 2120 1775 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4474 Fragments arranged by size: 4474 939 552 NdeI CA'TA_TG Cuts at: 387 2384 387 Size: 1997 3968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5965 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2385 1613 Size: 772 5193 PsiI TTA'TAA Cuts at: 3153 3535 3153 Size: 382 5583 PstI C_TGCA'G Cuts at: 830 2380 2545 3317 830 Size: 1550 165 772 3478 Fragments arranged by size: 3478 1550 772 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SapI GCTCTTCn'nnn_ Cuts at: 2951 2951 Size: 5965 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 2081 2903 4213 5925 2081 Size: 822 1310 1712 2121 Fragments arranged by size: 2121 1712 1310 822 SfcI C'TryA_G Cuts at: 826 1045 2376 2541 3313 4448 5129 5320 826 Size: 219 1331 165 772 1135 681 191 1471 Fragments arranged by size: 1471 1331 1135 772 681 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 4069 4970 5232 820 Size: 197 926 1293 833 901 262 1553 Fragments arranged by size: 1553 1293 926 901 833 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2004 2685 3404 513 Size: 552 939 681 719 3074 Fragments arranged by size: 3074 939 719 681 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3992 4009 4162 4347 4792 325 Size: 1255 2412 17 153 185 445 1498 Fragments arranged by size: 2412 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 2901 4211 Size: 274 80 33 51 1443 102 822 1310 Cuts at: 4211 5923 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1310 822 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 2625 2754 3195 4094 2625 Size: 129 441 899 4496 Fragments arranged by size: 4496 899 441 129 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI