Restriction map for
pFN21AB0958
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeIII MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 1231 CTTAAG C^TTAAG
AlwI 1027 GATCC GGATC(4/5)
AspHI 808 GAGCAC G[AT]GC[AT]^C
AvaI 28 CCCGAG C^[CT]CG[GA]G
BalI 410 TGGCCA TGG^CCA
BanII 954 GAGCCC G[GA]GC[CT]^C
BfaI 1162 CTAG C^TAG
BfrI 1231 CTTAAG C^TTAAG
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BsiHKAI 808 GAGCAC G[AT]GC[AT]^C
BsoBI 28 CCCGAG C^[CT]CG[GA]G
CfoI 242 GCGC GCG^C
DsaI 907 CCACGG C^C[GA][CT]GG
EaeI 410 TGGCCA [CT]^GGCC[GA]
EcoT14I 680 CCTTGG C^C[AT][AT]GG
HaeIII 411 GGCC GG^CC
HapII 51 CCGG C^CGG
HhaI 242 GCGC GCG^C
HinP1I 242 GCGC G^CGC
HpaII 51 CCGG C^CGG
MaeI 1162 CTAG C^TAG
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
MspI 51 CCGG C^CGG
NdeI 378 CATATG CA^TATG
PshAI 376 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 951 GAAGAGC GCTCTTC(1/4)
SmlI 1231 CTTAAG C^T[CT][GA]AG
StyI 680 CCTTGG C^C[AT][AT]GG
TaqI 701 TCGA T^CGA
TspRI 278 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 701 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 130 GAATTT [GA]^AATT[CT]
620 AAATTC
ApoI 130 GAATTT [GA]^AATT[CT]
620 AAATTC
BbsI 1152 GAAGAC GAAGAC(2/6)
531 GTCTTC
BbvI 24 GCTGC GCAGC(8/12)
33 GCTGC
BmyI 808 GAGCAC G[AGT]GC[ACT]^C
954 GAGCCC
BpmI 892 CTCCAG CTGGAG(16/14)
874 CTGGAG
BpuAI 1152 GAAGAC GAAGAC(2/6)
531 GTCTTC
BseRI 354 GAGGAG GAGGAG(10/8)
1102 GAGGAG
BsmBI 463 CGTCTC CGTCTC(1/5)
4 GAGACG
Bsp1286I 808 GAGCAC G[AGT]GC[ACT]^C
954 GAGCCC
BspHI 401 TCATGA T^CATGA
753 TCATGA
BssKI 297 CCAGG ^CC[ACGT]GG
869 CCTGG
BstF5I 970 CATCC GGATG(2/0)
1071 GGATG
BstNI 297 CCAGG CC^[AT]GG
869 CCTGG
Cac8I 83 GCTGGC GC[ACGT]^[ACGT]GC
826 GCCAGC
DpnII 193 GATC ^GATC
1027 GATC
EcoNI 570 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
973 CCTAAAACAGG
EcoRII 297 CCAGG ^CC[AT]GG
869 CCTGG
EcoRV 833 GATATC GAT^ATC
916 GATATC
Fnu4HI 24 GCTGC GC^[ACGT]GC
33 GCTGC
FokI 970 CATCC GGATG(9/13)
1071 GGATG
HindIII 771 AAGCTT A^AGCTT
1050 AAGCTT
HphI 606 TCACC GGTGA(8/7)
624 TCACC
ItaI 24 GCTGC GC^[ACGT]GC
33 GCTGC
MboI 193 GATC ^GATC
1027 GATC
MvaI 297 CCAGG CC^[AT]GG
869 CCTGG
NdeII 193 GATC ^GATC
1027 GATC
NlaIV 395 GGACCC GG[ACGT]^[ACGT]CC
865 GGACCC
PstI 371 CTGCAG CTGCA^G
536 CTGCAG
RcaI 401 TCATGA T^CATGA
753 TCATGA
Sau3AI 193 GATC ^GATC
1027 GATC
ScaI 74 AGTACT AGT^ACT
896 AGTACT
ScrFI 297 CCAGG CC^[ACGT]GG
869 CCTGG
SfcI 371 CTGCAG C^T[GA][CT]AG
536 CTGCAG
TseI 24 GCTGC G^C[AT]GC
33 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI Asp700I AvaII BsaJI
BsiYI BslI BsmAI BsrI Cfr13I
DdeI EarI HinfI Ksp632I MaeII
MboII MnlI MseI MslI MwoI
NlaIII RsaI Sau96I SfaNI TfiI
Tru9I Tsp509I XmnI