NUCB2 ORF clone pF1KE1237

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Flexi ORF Clone pF1KE1237

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Clone info.

Species

human

Clone Name

pF1KE1237 Sequence (with vector) Restriction Map

Product ID

FXC29636

HaloTag Clone PID

ORF Sequence

DNA sequence (1260 bp)
Protein sequence (420 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1237

Symbol

NUCB2

Alias : HEL-S-109, NEFA

Description

nucleobindin 2

Original Clone ID

cs03137

Note

We obtained this clone using the E.coli strain CopyCutterEPI400 at 25 deg C.

Features of the cloned ORF sequence

Description

Length: 1260 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 420 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41623.1 3.3e-86 100.0 NUCB2 CCDS81556.1 2.3e-62 92.9 NUCB2 CCDS12740.1 8.1e-49 61.4 NUCB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMSmart IPR002048 245 273 SM00054 Calcium-binding EF-hand IPR002048 297 325 SM00054 Calcium-binding EF-hand ProfileScan IPR018249 241 276 PS50222 EF-HAND 2 IPR018249 293 328 PS50222 EF-HAND 2
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 LLQYCFLLITCLLTALEAVPIDI 29 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016873304 420 608020 100.0 100.0 XP_016873298 420 608020 100.0 100.0 XP_016873303 420 608020 100.0 100.0 NP_005004 420 608020 100.0 100.0 XP_016873301 420 608020 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE1237 Download

>KIEE1237  1260 bp
ATGAGGTGGAGGACCATCCTGCTACAGTATTGCTTTCTCTTGATTACATGTTTACTTACT
GCTCTTGAAGCTGTGCCTATTGACATAGACAAGACAAAAGTACAAAATATTCACCCTGTG
GAAAGTGCGAAGATAGAACCACCAGATACTGGACTTTATTATGATGAATATCTCAAGCAA
GTGATTGATGTGCTGGAAACAGATAAACACTTCAGAGAAAAGCTCCAGAAAGCAGACATA
GAGGAAATAAAGAGTGGGAGGCTAAGCAAAGAACTGGATTTAGTAAGTCACCATGTGAGG
ACAAAACTTGATGAACTGAAAAGGCAAGAAGTAGGAAGGTTAAGAATGTTAATTAAAGCT
AAGTTGGATTCCCTTCAAGATATAGGCATGGACCACCAAGCTCTTCTAAAACAATTTGAT
CACCTAAACCACCTGAATCCTGACAAGTTTGAATCCACAGATTTAGATATGCTAATCAAA
GCGGCAACAAGTGATCTGGAACACTATGACAAGACTCGTCATGAAGAATTTAAAAAATAT
GAAATGATGAAGGAACATGAAAGGAGAGAATATTTAAAAACATTGAATGAAGAAAAGAGA
AAAGAAGAAGAGTCTAAATTTGAAGAAATGAAGAAAAAGCATGAAAATCACCCTAAAGTT
AATCACCCAGGAAGCAAAGATCAACTAAAAGAGGTATGGGAAGAGACTGATGGATTGGAT
CCTAATGACTTTGACCCCAAGACATTTTTCAAATTACATGATGTCAATAGTGATGGATTC
CTGGATGAACAAGAATTAGAAGCCCTATTTACTAAAGAGTTGGAGAAAGTATATGACCCT
AAAAATGAAGAGGATGATATGGTAGAAATGGAAGAAGAAAGGCTTAGAATGAGGGAACAT
GTAATGAATGAGGTTGATACTAACAAAGACAGATTGGTGACTCTGGAGGAGTTTTTGAAA
GCCACAGAAAAAAAAGAATTCTTGGAGCCAGATAGCTGGGAGACATTAGATCAGCAACAG
TTCTTCACAGAGGAAGAACTAAAAGAATATGAAAATATTATTGCTTTACAAGAAAATGAA
CTTAAGAAGAAGGCAGATGAGCTTCAGAAACAAAAAGAAGAGCTACAACGTCAGCATGAT
CAACTGGAGGCTCAGAAGCTGGAATATCATCAGGTCATACAGCAGATGGAACAAAAAAAA
TTACAACAAGGAATTCCTCCATCAGGGCCAGCTGGAGAATTGAAGTTTGAGCCACACATT

ORF Protein

Cloned ORF protein sequence for pF1KE1237 Download

>KIEE1237  420 aa
MRWRTILLQYCFLLITCLLTALEAVPIDIDKTKVQNIHPVESAKIEPPDTGLYYDEYLKQ
VIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTKLDELKRQEVGRLRMLIKA
KLDSLQDIGMDHQALLKQFDHLNHLNPDKFESTDLDMLIKAATSDLEHYDKTRHEEFKKY
EMMKEHERREYLKTLNEEKRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLD
PNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREH
VMNEVDTNKDRLVTLEEFLKATEKKEFLEPDSWETLDQQQFFTEEELKEYENIIALQENE
LKKKADELQKQKEELQRQHDQLEAQKLEYHQVIQQMEQKKLQQGIPPSGPAGELKFEPHI

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE1237 Download

>pF1KE1237  4373 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGAGGTGGAGG
ACCATCCTGCTACAGTATTGCTTTCTCTTGATTACATGTTTACTTACTGCTCTTGAAGCT
GTGCCTATTGACATAGACAAGACAAAAGTACAAAATATTCACCCTGTGGAAAGTGCGAAG
ATAGAACCACCAGATACTGGACTTTATTATGATGAATATCTCAAGCAAGTGATTGATGTG
CTGGAAACAGATAAACACTTCAGAGAAAAGCTCCAGAAAGCAGACATAGAGGAAATAAAG
AGTGGGAGGCTAAGCAAAGAACTGGATTTAGTAAGTCACCATGTGAGGACAAAACTTGAT
GAACTGAAAAGGCAAGAAGTAGGAAGGTTAAGAATGTTAATTAAAGCTAAGTTGGATTCC
CTTCAAGATATAGGCATGGACCACCAAGCTCTTCTAAAACAATTTGATCACCTAAACCAC
CTGAATCCTGACAAGTTTGAATCCACAGATTTAGATATGCTAATCAAAGCGGCAACAAGT
GATCTGGAACACTATGACAAGACTCGTCATGAAGAATTTAAAAAATATGAAATGATGAAG
GAACATGAAAGGAGAGAATATTTAAAAACATTGAATGAAGAAAAGAGAAAAGAAGAAGAG
TCTAAATTTGAAGAAATGAAGAAAAAGCATGAAAATCACCCTAAAGTTAATCACCCAGGA
AGCAAAGATCAACTAAAAGAGGTATGGGAAGAGACTGATGGATTGGATCCTAATGACTTT
GACCCCAAGACATTTTTCAAATTACATGATGTCAATAGTGATGGATTCCTGGATGAACAA
GAATTAGAAGCCCTATTTACTAAAGAGTTGGAGAAAGTATATGACCCTAAAAATGAAGAG
GATGATATGGTAGAAATGGAAGAAGAAAGGCTTAGAATGAGGGAACATGTAATGAATGAG
GTTGATACTAACAAAGACAGATTGGTGACTCTGGAGGAGTTTTTGAAAGCCACAGAAAAA
AAAGAATTCTTGGAGCCAGATAGCTGGGAGACATTAGATCAGCAACAGTTCTTCACAGAG
GAAGAACTAAAAGAATATGAAAATATTATTGCTTTACAAGAAAATGAACTTAAGAAGAAG
GCAGATGAGCTTCAGAAACAAAAAGAAGAGCTACAACGTCAGCATGATCAACTGGAGGCT
CAGAAGCTGGAATATCATCAGGTCATACAGCAGATGGAACAAAAAAAATTACAACAAGGA
ATTCCTCCATCAGGGCCAGCTGGAGAATTGAAGTTTGAGCCACACATTGTTTAAACGAAT
TCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGC
TGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC
ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTAT
ATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACT
GCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCT
GACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCT
TTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCT
GGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCG
CCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTT
CGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA
TTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTG
TCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAA
CTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCT
GTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGG
CAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCA
ATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACAT
CGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGAC
GAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCG
GATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAA
AATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAG
GACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGC
TTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTT
CTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCA
ACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT
GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAG
TCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC
CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC
TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT
CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGC
AGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAA
GCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG
TAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGA
AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG
GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAA
TACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGC
TTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATAC
GGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTAC
CTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTAT
GGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCA
CGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACG
CAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGA
CCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCA
TGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGG
CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGG
GAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCAT
AAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTC
TACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT