################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BglII BlnI BmyI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TseI TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 481 GCGG CCGC(-3/-1) AfaI 100 GTAC GT^AC AflII 1081 CTTAAG C^TTAAG BamHI 717 GGATCC G^GATCC BfrI 1081 CTTAAG C^TTAAG BlpI 261 GCTAAGC GC^T[ACGT]AGC Bpu1102I 261 GCTAAGC GC^T[ACGT]AGC BsaBI 163 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 666 CCCAGG C^C[ACGT][ACGT]GG BseRI 946 GAGGAG GAGGAG(10/8) BspHI 519 TCATGA T^CATGA BstXI 988 CCAGATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 717 GGATCC [GA]^GATC[CT] Cac8I 1227 GCCAGC GC[ACGT]^[ACGT]GC CelII 261 GCTAAGC GC^T[ACGT]AGC EcoNI 1216 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 481 GCGGC GC^[ACGT]GC HaeIII 1226 GGCC GG^CC ItaI 481 GCGGC GC^[ACGT]GC MaeII 1128 ACGT A^CGT MamI 163 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 717 GGATCC [GA]^GATC[CT] MspA1I 1229 CAGCTG C[AC]G^C[GT]G MwoI 61 GCTCTTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PacI 349 TTAATTAA TTAAT^TAA PflMI 142 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1229 CAGCTG CAG^CTG RcaI 519 TCATGA T^CATGA RsaI 100 GTAC GT^AC SfcI 22 CTACAG C^T[GA][CT]AG Van91I 142 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 717 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 46 ACATGT A^C[GA][CT]GT 897 ACATGT AlwI 718 GATCC GGATC(4/5) 717 GGATC AlwNI 143 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 1153 CAGAAGCTG AvaII 11 GGACC G^G[AT]CC 390 GGACC BclI 417 TGATCA T^GATCA 1137 TGATCA BsiYI 142 CCAGATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1216 CCTCCATCAGG BslI 142 CCAGATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1216 CCTCCATCAGG BsmAI 703 GAGAC GTCTC(1/5) 1000 GAGAC BspLU11I 46 ACATGT A^CATGT 897 ACATGT BssKI 667 CCAGG ^CC[ACGT]GG 780 CCTGG BstNI 667 CCAGG CC^[AT]GG 780 CCTGG DraI 529 TTTAAA TTT^AAA 573 TTTAAA DraIII 112 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 289 CACCATGTG EcoRI 976 GAATTC G^AATTC 1211 GAATTC EcoRII 667 CCAGG ^CC[AT]GG 780 CCTGG FbaI 417 TGATCA T^GATCA 1137 TGATCA MaeIII 287 GTCAC ^GT[ACGT]AC 937 GTGAC MvaI 667 CCAGG CC^[AT]GG 780 CCTGG NlaIV 717 GGATCC GG[ACGT]^[ACGT]CC 984 GGAGCC NspI 46 ACATGT [GA]CATG^[CT] 897 ACATGT SapI 1117 GAAGAGC GCTCTTC(1/4) 400 GCTCTTC ScrFI 667 CCAGG CC^[ACGT]GG 780 CCTGG SmlI 172 CTCAAG C^T[CT][GA]AG 1081 CTTAAG Tsp45I 287 GTCAC ^GT[GC]AC 937 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AluI ApoI BpmI BsrI BstF5I Cfr13I DdeI DpnII EarI FokI HinfI HphI Ksp632I MboI MboII MnlI MseI NdeII NlaIII PleI Sau3AI Sau96I SspI TfiI Tru9I Tsp509I