(Circular) (Six-base) MAPSORT of: pF1KE1237.seq Check: 1717 from: 1 to: 4373 pF1KE1237 4373 bp With 182 enzymes: SgfI * November 2, 2015 11:21 .. AccI GT'mk_AC Cuts at: 1411 1411 Size: 4373 Acc65I G'GTAC_C Cuts at: 1389 1389 Size: 4373 AclI AA'CG_TT Cuts at: 4213 4213 Size: 4373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 303 1195 2198 2630 3377 3645 303 Size: 892 1003 432 747 268 1031 Fragments arranged by size: 1031 1003 892 747 432 268 AflII C'TTAA_G Cuts at: 1189 1189 Size: 4373 AflIII A'CryG_T Cuts at: 154 1005 1725 2829 3806 154 Size: 851 720 1104 977 721 Fragments arranged by size: 1104 977 851 721 720 AgeI A'CCGG_T Cuts at: 2761 2761 Size: 4373 AlwNI CAG_nnn'CTG Cuts at: 256 1266 1678 3245 256 Size: 1010 412 1567 1384 Fragments arranged by size: 1567 1384 1010 412 ApaLI G'TGCA_C Cuts at: 3143 3143 Size: 4373 ApoI r'AATT_y Cuts at: 634 724 1084 1319 1377 3726 3982 634 Size: 90 360 235 58 2349 256 1025 Fragments arranged by size: 2349 1025 360 256 235 90 58 AseI AT'TA_AT Cuts at: 20 20 Size: 4373 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4373 AvaI C'yCGr_G Cuts at: 1393 1393 Size: 4373 BamHI G'GATC_C Cuts at: 825 1398 825 Size: 573 3800 BanI G'GyrC_C Cuts at: 1389 1803 2051 2086 1389 Size: 414 248 35 3676 Fragments arranged by size: 3676 414 248 35 BanII G_rGCy'C Cuts at: 1387 2417 1387 Size: 1030 3343 BbeI G_GCGC'C Cuts at: 1807 2055 1807 Size: 248 4125 BciVI GTATCCnnnnn_n' Cuts at: 2271 3032 4373 2271 Size: 761 1341 2271 Fragments arranged by size: 2271 1341 761 BclI T'GATC_A Cuts at: 525 1245 1893 525 Size: 720 648 3005 Fragments arranged by size: 3005 720 648 BglII A'GATC_T Cuts at: 1888 3925 1888 Size: 2037 2336 BlpI GC'TnA_GC Cuts at: 370 1485 370 Size: 1115 3258 Bme1580I G_kGCm'C Cuts at: 1998 2091 3147 1998 Size: 93 1056 3224 Fragments arranged by size: 3224 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1585 1666 2001 4145 1585 Size: 81 335 2144 1813 Fragments arranged by size: 2144 1813 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 346 2740 346 Size: 2394 1979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 316 1072 1273 1361 316 Size: 756 201 88 3328 Fragments arranged by size: 3328 756 201 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 265 1549 2428 2920 3218 265 Size: 1284 879 492 298 1420 Fragments arranged by size: 1420 1284 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4077 4077 Size: 4373 BsaAI yAC'GTr Cuts at: 1726 1726 Size: 4373 BsaBI GATnn'nnATC Cuts at: 6 275 1887 3924 6 Size: 269 1612 2037 455 Fragments arranged by size: 2037 1612 455 269 BsaHI Gr'CG_yC Cuts at: 1804 2052 2754 4250 1804 Size: 248 702 1496 1927 Fragments arranged by size: 1927 1496 702 248 BsaWI w'CCGG_w Cuts at: 1562 2083 2761 3035 3182 3593 1562 Size: 521 678 274 147 411 2342 Fragments arranged by size: 2342 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1069 1069 Size: 4373 BseYI C'CCAG_C Cuts at: 1103 1798 2556 3133 3629 1103 Size: 695 758 577 496 1847 Fragments arranged by size: 1847 758 695 577 496 BsiEI CG_ry'CG Cuts at: 104 1915 1961 3169 104 Size: 1811 46 1208 1308 Fragments arranged by size: 1811 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1387 2165 2355 3147 1387 Size: 778 190 792 2613 Fragments arranged by size: 2613 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1387 1998 2091 2165 2355 2417 3147 1387 Size: 611 93 74 190 62 730 2613 Fragments arranged by size: 2613 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3593 3593 Size: 4373 BspHI T'CATG_A Cuts at: 627 3549 627 Size: 2922 1451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1423 1637 1939 2320 1423 Size: 214 302 381 3476 Fragments arranged by size: 3476 381 302 214 BsrBI CCG'CTC Cuts at: 50 2665 2719 4203 4368 50 Size: 2615 54 1484 165 55 Fragments arranged by size: 2615 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2285 2285 Size: 4373 BsrFI r'CCGG_y Cuts at: 2371 2552 2761 2371 Size: 181 209 3983 Fragments arranged by size: 3983 209 181 BssSI C'ACGA_G Cuts at: 2644 3002 2644 Size: 358 4015 BstAPI GCAn_nnn'nTGC Cuts at: 1750 1750 Size: 4373 BstBI TT'CG_AA Cuts at: 2734 2734 Size: 4373 BstXI CCAn_nnnn'nTGG Cuts at: 1103 1103 Size: 4373 BstYI r'GATC_y Cuts at: 1 825 1398 1865 1888 2223 2469 3481 3567 Size: 824 573 467 23 335 246 1012 86 Cuts at: 3567 3579 3925 1 Size: 12 346 449 Fragments arranged by size: 1012 824 573 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2323 2504 2667 2323 Size: 181 163 4029 Fragments arranged by size: 4029 181 163 BtsI GCAGTG_nn' Cuts at: 1616 1616 Size: 4373 DraI TTT'AAA Cuts at: 639 683 1372 639 Size: 44 689 3640 Fragments arranged by size: 3640 689 44 DraIII CAC_nnn'GTG Cuts at: 225 402 225 Size: 177 4196 DrdI GACnn_nn'nnGTC Cuts at: 2079 2937 2079 Size: 858 3515 EaeI y'GGCC_r Cuts at: 1958 2132 2523 2550 3895 1958 Size: 174 391 27 1345 2436 Fragments arranged by size: 2436 1345 391 174 27 EagI C'GGCC_G Cuts at: 1958 1958 Size: 4373 EarI CTCTTCn'nnn_ Cuts at: 515 710 803 950 1220 2396 2606 515 Size: 195 93 147 270 1176 210 2282 Fragments arranged by size: 2282 1176 270 210 195 147 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2581 2891 3037 4183 2581 Size: 310 146 1146 2771 Fragments arranged by size: 2771 1146 310 146 EcoICRI GAG'CTC Cuts at: 1385 1385 Size: 4373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 303 316 1072 1195 1273 1361 2198 2630 3377 Size: 13 756 123 78 88 837 432 747 Cuts at: 3377 3645 303 Size: 268 1031 Fragments arranged by size: 1031 837 756 747 432 268 123 88 78 13 EcoNI CCTnn'n_nnAGG Cuts at: 1328 1328 Size: 4373 EcoO109I rG'GnC_Cy Cuts at: 1512 1512 Size: 4373 EcoRI G'AATT_C Cuts at: 1084 1319 1377 1084 Size: 235 58 4080 Fragments arranged by size: 4080 235 58 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1006 2392 1006 Size: 1386 2987 FspI TGC'GCA Cuts at: 2154 2154 Size: 4373 HaeII r_GCGC'y Cuts at: 1807 2055 3077 4056 1807 Size: 248 1022 979 2124 Fragments arranged by size: 2124 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1101 1133 2215 2245 2247 2277 1101 Size: 32 1082 30 2 30 3197 Fragments arranged by size: 3197 1082 32 30 30 2 HincII GTy'rAC Cuts at: 1412 1412 Size: 4373 KasI G'GCGC_C Cuts at: 1803 2051 1803 Size: 248 4125 KpnI G_GTAC'C Cuts at: 1393 1393 Size: 4373 MluI A'CGCG_T Cuts at: 3806 3806 Size: 4373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 452 908 3044 3228 452 Size: 456 2136 184 1597 Fragments arranged by size: 2136 1597 456 184 MscI TGG'CCA Cuts at: 2134 2134 Size: 4373 MslI CAynn'nnrTG Cuts at: 2362 2489 2362 Size: 127 4246 MspA1I CmG'CkG Cuts at: 1339 1484 1798 2158 3171 3416 1339 Size: 145 314 360 1013 245 2296 Fragments arranged by size: 2296 1013 360 314 245 145 NaeI GCC'GGC Cuts at: 2554 2554 Size: 4373 NarI GG'CG_CC Cuts at: 1804 2052 1804 Size: 248 4125 NgoMIV G'CCGG_C Cuts at: 2552 2552 Size: 4373 NspI r_CATG'y Cuts at: 158 1009 1426 1926 2833 158 Size: 851 417 500 907 1698 Fragments arranged by size: 1698 907 851 500 417 PacI TTA_AT'TAA Cuts at: 461 461 Size: 4373 PciI A'CATG_T Cuts at: 154 1005 2829 154 Size: 851 1824 1698 Fragments arranged by size: 1824 1698 851 PflMI CCAn_nnn'nTGG Cuts at: 113 256 113 Size: 143 4230 PfoI T'CCnGG_A Cuts at: 887 887 Size: 4373 PmeI GTTT'AAAC Cuts at: 1372 1372 Size: 4373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 3512 Size: 32 4341 PstI C_TGCA'G Cuts at: 1420 2105 1420 Size: 685 3688 PvuI CG_AT'CG Cuts at: 104 104 Size: 4373 PvuII CAG'CTG Cuts at: 1339 1798 2158 1339 Size: 459 360 3554 Fragments arranged by size: 3554 459 360 RsrII CG'GwC_CG Cuts at: 2568 2568 Size: 4373 SacI G_AGCT'C Cuts at: 1387 1387 Size: 4373 SalI G'TCGA_C Cuts at: 1410 1410 Size: 4373 SapI GCTCTTCn'nnn_ Cuts at: 515 1220 2396 2606 515 Size: 705 1176 210 2282 Fragments arranged by size: 2282 1176 705 210 SbfI CC_TGCA'GG Cuts at: 1420 1420 Size: 4373 ScaI AGT'ACT Cuts at: 3890 3890 Size: 4373 SfcI C'TryA_G Cuts at: 33 130 1416 2101 3094 3285 33 Size: 97 1286 685 993 191 1121 Fragments arranged by size: 1286 1121 993 685 191 97 SfoI GGC'GCC Cuts at: 1805 2053 1805 Size: 248 4125 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4373 SmaI CCC'GGG Cuts at: 1395 1395 Size: 4373 SmlI C'TyrA_G Cuts at: 280 1189 1528 2443 2935 3197 280 Size: 909 339 915 492 262 1456 Fragments arranged by size: 1456 915 909 492 339 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4373 SphI G_CATG'C Cuts at: 1426 1926 1426 Size: 500 3873 SspI AAT'ATT Cuts at: 216 679 1164 216 Size: 463 485 3425 Fragments arranged by size: 3425 485 463 StyI C'CwwG_G Cuts at: 1507 1861 1507 Size: 354 4019 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 788 1956 2089 2548 2757 788 Size: 1168 133 459 209 2404 Fragments arranged by size: 2404 1168 459 209 133 TatI w'GTAC_w Cuts at: 207 3888 207 Size: 3681 692 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3890 3922 3890 Size: 32 4341 Tth111I GACn'n_nGTC Cuts at: 2170 2170 Size: 4373 XbaI T'CTAG_A Cuts at: 1404 1404 Size: 4373 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1868 1868 Size: 4373 XmaI C'CCGG_G Cuts at: 1393 1393 Size: 4373 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PacI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI