FADS3 ORF clone FHC11678

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11678

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Clone info.

Species

human

Clone Name

pFN21AE1051 Sequence (with vector) Restriction Map

Product ID

FHC11678

Flexi Clone PID

FXC11678

ORF Sequence

DNA sequence (1335 bp)
Protein sequence (445 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529172]

Gene

KIEE1051

Symbol

FADS3

Alias : CYB5RP, LLCDL3

Description

fatty acid desaturase 3

Original Clone ID

cp01518

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1335 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 445 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8013.1 9.4e-200 100.0 FADS3 CCDS8012.1 9.5e-128 62.6 FADS2 CCDS60808.1 9e-111 64.2 FADS2 CCDS60807.1 9.2e-111 63.0 FADS2 CCDS8011.2 1.4e-103 52.4 FADS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001199 23 96 PF00173 Cytochrome b5 IPR005804 159 415 PF00487 Fatty acid desaturase ProfileScan IPR001199 20 97 PS50255 Cytochrome b5
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 130 ASPTFFAFLLGHILAMEVLAWLL 152 PRIMARY 23 2 158 PGWVPSALAAFILAISQAQSWCL 180 SECONDARY 23 3 268 FLIGPPLLTLVNFEVENLAYMLV 290 SECONDARY 23 4 309 LSYLPFYGVPGVLLFFVAVRVLE 331 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_068373 445 606150 100.0 100.0 XP_011543325 454 606150 97.8 100.0 XP_016873213 491 606150 97.4 87.2 XP_016873212 500 606150 95.0 87.2 NP_004256 444 606149 62.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1051 Download

>KIEE1051  1335 bp
ATGGGCGGCGTCGGGGAGCCGGGACCGCGGGAGGGACCCGCGCAGCCGGGGGCACCGCTG
CCCACCTTCTGCTGGGAGCAGATCCGCGCGCACGACCAGCCCGGCGACAAGTGGCTGGTC
ATCGAGCGCCGCGTCTACGACATCAGCCGCTGGGCACAGCGGCACCCAGGGGGCAGCCGC
CTCATCGGCCACCACGGCGCTGAGGACGCCACGGATGCCTTCCGTGCCTTCCATCAAGAT
CTCAATTTTGTGCGCAAGTTCCTACAGCCCCTGTTGATTGGAGAGCTGGCTCCGGAAGAA
CCCAGCCAGGATGGACCCCTGAATGCGCAGCTGGTCGAGGACTTCCGAGCCCTGCACCAG
GCAGCCGAGGACATGAAGCTGTTTGATGCCAGTCCCACCTTCTTTGCTTTCCTACTGGGC
CACATCCTGGCCATGGAGGTGCTGGCCTGGCTCCTTATCTACCTCCTGGGTCCTGGCTGG
GTGCCCAGTGCCCTGGCCGCCTTCATCCTGGCCATCTCTCAGGCTCAGTCCTGGTGTCTG
CAGCATGACCTGGGCCATGCCTCCATCTTCAAGAAGTCCTGGTGGAACCACGTGGCCCAG
AAGTTCGTGATGGGGCAGCTAAAGGGCTTCTCCGCCCACTGGTGGAACTTCCGCCACTTC
CAGCACCACGCCAAGCCCAACATCTTCCACAAAGACCCAGACGTGACGGTGGCGCCCGTC
TTCCTCCTGGGGGAGTCATCCGTCGAGTATGGCAAGAAGAAACGCAGATACCTACCCTAC
AACCAGCAGCACCTGTACTTCTTCCTGATCGGCCCGCCGCTGCTCACCCTGGTGAACTTT
GAAGTGGAAAATCTGGCGTACATGCTGGTGTGCATGCAGTGGGCGGATTTGCTCTGGGCC
GCCAGCTTCTATGCCCGCTTCTTCTTATCCTACCTCCCCTTCTACGGCGTCCCTGGGGTG
CTGCTCTTCTTTGTTGCTGTCAGGGTCCTGGAAAGCCACTGGTTCGTGTGGATCACACAG
ATGAACCACATCCCCAAGGAGATCGGCCACGAGAAGCACCGGGACTGGGTCAGCTCTCAG
CTGGCAGCCACCTGCAACGTGGAGCCCTCACTTTTCACCAACTGGTTCAGCGGGCACCTC
AACTTCCAGATCGAGCACCACCTCTTCCCCAGGATGCCGAGACACAACTACAGCCGGGTG
GCCCCGCTGGTCAAGTCGCTGTGTGCCAAGCACGGCCTCAGCTACGAAGTGAAGCCCTTC
CTCACCGCGCTGGTGGACATCGTCAGGTCCCTGAAGAAGTCTGGTGACATCTGGCTGGAC
GCCTACCTCCATCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE1051 Download

>KIEE1051  445 aa
MGGVGEPGPREGPAQPGAPLPTFCWEQIRAHDQPGDKWLVIERRVYDISRWAQRHPGGSR
LIGHHGAEDATDAFRAFHQDLNFVRKFLQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQ
AAEDMKLFDASPTFFAFLLGHILAMEVLAWLLIYLLGPGWVPSALAAFILAISQAQSWCL
QHDLGHASIFKKSWWNHVAQKFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPV
FLLGESSVEYGKKKRRYLPYNQQHLYFFLIGPPLLTLVNFEVENLAYMLVCMQWADLLWA
ASFYARFFLSYLPFYGVPGVLLFFVAVRVLESHWFVWITQMNHIPKEIGHEKHRDWVSSQ
LAATCNVEPSLFTNWFSGHLNFQIEHHLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKPF
LTALVDIVRSLKKSGDIWLDAYLHQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1051 Download

>pFN21AE1051  6040 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCGGCGTCGGGGAGCCGGGACCGCGGGAGGG
ACCCGCGCAGCCGGGGGCACCGCTGCCCACCTTCTGCTGGGAGCAGATCCGCGCGCACGA
CCAGCCCGGCGACAAGTGGCTGGTCATCGAGCGCCGCGTCTACGACATCAGCCGCTGGGC
ACAGCGGCACCCAGGGGGCAGCCGCCTCATCGGCCACCACGGCGCTGAGGACGCCACGGA
TGCCTTCCGTGCCTTCCATCAAGATCTCAATTTTGTGCGCAAGTTCCTACAGCCCCTGTT
GATTGGAGAGCTGGCTCCGGAAGAACCCAGCCAGGATGGACCCCTGAATGCGCAGCTGGT
CGAGGACTTCCGAGCCCTGCACCAGGCAGCCGAGGACATGAAGCTGTTTGATGCCAGTCC
CACCTTCTTTGCTTTCCTACTGGGCCACATCCTGGCCATGGAGGTGCTGGCCTGGCTCCT
TATCTACCTCCTGGGTCCTGGCTGGGTGCCCAGTGCCCTGGCCGCCTTCATCCTGGCCAT
CTCTCAGGCTCAGTCCTGGTGTCTGCAGCATGACCTGGGCCATGCCTCCATCTTCAAGAA
GTCCTGGTGGAACCACGTGGCCCAGAAGTTCGTGATGGGGCAGCTAAAGGGCTTCTCCGC
CCACTGGTGGAACTTCCGCCACTTCCAGCACCACGCCAAGCCCAACATCTTCCACAAAGA
CCCAGACGTGACGGTGGCGCCCGTCTTCCTCCTGGGGGAGTCATCCGTCGAGTATGGCAA
GAAGAAACGCAGATACCTACCCTACAACCAGCAGCACCTGTACTTCTTCCTGATCGGCCC
GCCGCTGCTCACCCTGGTGAACTTTGAAGTGGAAAATCTGGCGTACATGCTGGTGTGCAT
GCAGTGGGCGGATTTGCTCTGGGCCGCCAGCTTCTATGCCCGCTTCTTCTTATCCTACCT
CCCCTTCTACGGCGTCCCTGGGGTGCTGCTCTTCTTTGTTGCTGTCAGGGTCCTGGAAAG
CCACTGGTTCGTGTGGATCACACAGATGAACCACATCCCCAAGGAGATCGGCCACGAGAA
GCACCGGGACTGGGTCAGCTCTCAGCTGGCAGCCACCTGCAACGTGGAGCCCTCACTTTT
CACCAACTGGTTCAGCGGGCACCTCAACTTCCAGATCGAGCACCACCTCTTCCCCAGGAT
GCCGAGACACAACTACAGCCGGGTGGCCCCGCTGGTCAAGTCGCTGTGTGCCAAGCACGG
CCTCAGCTACGAAGTGAAGCCCTTCCTCACCGCGCTGGTGGACATCGTCAGGTCCCTGAA
GAAGTCTGGTGACATCTGGCTGGACGCCTACCTCCATCAGGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT