(Circular) (Six-base) MAPSORT of: pFN21AE1051.seq Check: 374 from: 1 to: 6040 pFN21AE1051 6040 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3104 3104 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 3383 1937 Size: 202 1244 4594 Fragments arranged by size: 4594 1244 202 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3317 4103 5487 5755 1632 Size: 1685 786 1384 268 1917 Fragments arranged by size: 1917 1685 1384 786 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 2834 1166 Size: 1668 4372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3112 1184 Size: 1928 4112 AlwNI CAG_nnn'CTG Cuts at: 2154 2275 2797 3783 5355 2154 Size: 121 522 986 1572 2839 Fragments arranged by size: 2839 1572 986 522 121 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3365 1870 Size: 73 1422 4545 Fragments arranged by size: 4545 1422 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2166 2485 2716 3138 Size: 325 217 627 269 110 319 231 422 Cuts at: 3138 3361 4816 618 Size: 223 1455 1842 Fragments arranged by size: 1842 1455 627 422 325 319 269 231 223 217 110 BanII G_rGCy'C Cuts at: 1391 2356 3111 3359 1391 Size: 965 755 248 4072 Fragments arranged by size: 4072 965 755 248 BbeI G_GCGC'C Cuts at: 2720 2720 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2716 928 Size: 971 817 4252 Fragments arranged by size: 4252 971 817 BbvCI CC'TCA_GC Cuts at: 2205 3242 2205 Size: 1037 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2190 2199 4651 Size: 107 122 71 631 605 518 9 2452 Cuts at: 4651 136 Size: 1525 Fragments arranged by size: 2452 1525 631 605 518 122 107 71 9 BglII A'GATC_T Cuts at: 2242 6035 2242 Size: 3793 2247 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2059 2161 2490 2497 3141 4101 5257 1790 Size: 269 102 329 7 644 960 1156 2573 Fragments arranged by size: 2573 1156 960 644 329 269 102 7 BmgBI CAC'GTC Cuts at: 1318 2707 1318 Size: 1389 4651 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2429 2484 3079 Size: 416 53 83 399 24 1012 55 595 Cuts at: 3079 3771 4729 442 Size: 692 958 1753 Fragments arranged by size: 1753 1012 958 692 595 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 Bpu10I CC'TnA_GC Cuts at: 2205 3242 2205 Size: 1037 5003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 3831 493 Size: 711 1392 1235 2702 Fragments arranged by size: 2702 1392 1235 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2211 2717 2952 Size: 53 83 186 936 480 198 506 235 Cuts at: 2952 3324 3847 4229 4864 275 Size: 372 523 382 635 1451 Fragments arranged by size: 1451 936 635 523 506 480 382 372 235 198 186 83 53 BsaWI w'CCGG_w Cuts at: 2296 4473 4871 5145 5292 5703 2296 Size: 2177 398 274 147 411 2633 Fragments arranged by size: 2633 2177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 2076 2154 2306 2481 5243 5739 2076 Size: 78 152 175 2762 496 2377 Fragments arranged by size: 2762 2377 496 175 152 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 1163 Size: 573 606 4861 Fragments arranged by size: 4861 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3162 4101 4186 5257 3162 Size: 939 85 1071 3945 Fragments arranged by size: 3945 1071 939 85 BsmI GAATG_Cn' Cuts at: 2332 3551 3644 2332 Size: 1219 93 4728 Fragments arranged by size: 4728 1219 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2161 2356 2490 2497 3111 3141 Size: 399 269 102 195 134 7 614 30 Cuts at: 3141 3162 3359 4101 4186 5257 1391 Size: 21 197 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 399 269 197 195 134 102 85 30 21 7 BspEI T'CCGG_A Cuts at: 2296 5703 2296 Size: 3407 2633 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3104 3395 844 Size: 2260 291 3489 Fragments arranged by size: 3489 2260 291 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 2091 1925 Size: 166 5874 BssSI C'ACGA_G Cuts at: 3053 4100 5112 3053 Size: 1047 1012 3981 Fragments arranged by size: 3981 1047 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstXI CCAn_nnnn'nTGG Cuts at: 2497 2497 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2085 2242 3370 3737 4122 4139 5591 5677 Size: 113 157 1128 367 385 17 1452 86 Cuts at: 5677 5689 6035 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1128 385 367 346 157 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 2197 2214 2436 513 Size: 552 939 26 167 17 222 4117 Fragments arranged by size: 4117 939 552 222 167 26 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2888 3556 4350 4370 2888 Size: 668 794 20 4558 Fragments arranged by size: 4558 794 668 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2389 2424 529 Size: 35 1825 35 4145 Fragments arranged by size: 4145 1825 35 35 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 2433 2499 Size: 54 1425 50 275 18 361 242 66 Cuts at: 2499 2514 3049 3487 4376 6005 8 Size: 15 535 438 889 1629 43 Fragments arranged by size: 1629 1425 889 535 438 361 275 242 66 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2975 3173 3970 1353 Size: 1622 198 797 3423 Fragments arranged by size: 3423 1622 797 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2626 2645 2903 4627 5001 5147 Size: 1064 138 1037 19 258 1724 374 146 Cuts at: 5147 387 Size: 1280 Fragments arranged by size: 1724 1280 1064 1037 374 258 146 138 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3317 4103 4700 5487 5755 1239 Size: 393 1685 786 597 787 268 1524 Fragments arranged by size: 1685 1524 787 786 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 3335 2360 Size: 975 5065 EcoO109I rG'GnC_Cy Cuts at: 2039 2474 2989 3291 2039 Size: 435 515 302 4788 Fragments arranged by size: 4788 515 435 302 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 2258 2331 4546 2258 Size: 73 2215 3752 Fragments arranged by size: 3752 2215 73 HaeII r_GCGC'y Cuts at: 1678 2134 2205 2720 5187 1678 Size: 456 71 515 2467 2531 Fragments arranged by size: 2531 2467 515 456 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4681 4713 4755 4787 1373 Size: 32 135 32 3109 32 42 32 2626 Fragments arranged by size: 3109 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 2716 2716 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 2516 10 Size: 54 1425 343 603 81 3534 Fragments arranged by size: 3534 1425 603 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 2834 2870 3023 3998 4357 4516 Size: 648 1275 393 36 153 975 359 159 Cuts at: 4516 518 Size: 2042 Fragments arranged by size: 2042 1275 975 648 393 359 159 153 36 MspA1I CmG'CkG Cuts at: 1606 2032 2062 2154 2164 2335 2824 3085 3135 Size: 426 30 92 10 171 489 261 50 Cuts at: 3135 3211 3456 4133 5281 5526 1606 Size: 76 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 426 261 245 245 171 92 76 50 30 10 NarI GG'CG_CC Cuts at: 2717 2717 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 2004 2436 513 Size: 552 939 432 4117 Fragments arranged by size: 4117 939 552 432 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2869 2881 3398 4943 1132 Size: 97 1640 12 517 1545 2229 Fragments arranged by size: 2229 1640 1545 517 97 12 PasI CC'CwG_GG Cuts at: 1348 2171 2957 1348 Size: 823 786 4431 Fragments arranged by size: 4431 823 786 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2431 1108 Size: 1323 4717 PfoI T'CCnGG_A Cuts at: 2991 2991 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PmlI CAC'GTG Cuts at: 2596 2596 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2039 2474 2989 3291 2039 Size: 435 515 302 4788 Fragments arranged by size: 4788 515 435 302 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 2547 3392 830 Size: 1717 845 3478 Fragments arranged by size: 3478 1717 845 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2335 3085 2335 Size: 750 5290 SacII CC_GC'GG Cuts at: 2033 2033 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SanDI GG'GwC_CC Cuts at: 2039 2039 Size: 6040 SapI GCTCTTCn'nnn_ Cuts at: 2975 2975 Size: 6040 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2267 2543 3193 3388 4523 5204 5395 Size: 219 1222 276 650 195 1135 681 191 Cuts at: 5395 826 Size: 1471 Fragments arranged by size: 1471 1222 1135 681 650 276 219 195 191 SfoI GGC'GCC Cuts at: 2718 2718 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 2881 3398 1132 Size: 1749 517 3774 Fragments arranged by size: 3774 1749 517 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 3039 3479 513 Size: 552 939 432 603 440 3074 Fragments arranged by size: 3074 939 603 552 440 432 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2184 2473 4067 4084 4237 4422 4867 Size: 1255 604 289 1594 17 153 185 445 Cuts at: 4867 325 Size: 1498 Fragments arranged by size: 1594 1498 1255 604 445 289 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 4286 5998 Size: 274 80 33 51 1443 822 1487 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 1712 1487 1443 822 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 3071 3284 1316 Size: 1755 213 4072 Fragments arranged by size: 4072 1755 213 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 3100 2443 Size: 657 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 3260 4169 3260 Size: 909 5131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI