MFAP3 ORF clone FHC11544

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11544

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Clone info.

Species

human

Clone Name

pFN21AB4707 Sequence (with vector) Restriction Map

Product ID

FHC11544

Flexi Clone PID

FXC11544

ORF Sequence

DNA sequence (1086 bp)
Protein sequence (362 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB529114]

Gene

KIBB4707

Symbol

MFAP3

Description

microfibrillar-associated protein 3, transcript variant 1

Original Clone ID

fk15507

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1086 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 362 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4324.1 4.4e-142 100.0 MFAP3 CCDS47319.1 4.1e-82 100.0 MFAP3 CCDS34103.1 1.8e-57 55.1 MFAP3L CCDS43281.1 1.2e-51 65.1 MFAP3L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013098 61 130 PF07679 Immunoglobulin I-set HMMSmart IPR003599 58 141 SM00409 Immunoglobulin subtype IPR003598 64 131 SM00408 Immunoglobulin subtype 2 ProfileScan IPR007110 45 137 PS50835 Immunoglobulin-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 CCLFTLVASIIVPAAFVLEDVDF 27 PRIMARY 23 2 118 DRGLYTCFVTSPIRASYSVTLRV 140 SECONDARY 23 3 151 YMIVCLIAFTITLILNVTRLCMM 173 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001229265 362 600491 100.0 100.0 NP_005918 362 600491 100.0 100.0 XP_016864359 409 616523 55.1 86.2 NP_067679 409 616523 55.1 86.2 XP_016864358 409 616523 55.1 86.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4707 Download

>KIBB4707  1086 bp
ATGAAGCTACATTGTTGCTTATTCACTTTAGTGGCAAGTATTATTGTGCCAGCTGCTTTT
GTTTTGGAAGATGTGGACTTCGACCAAATGGTTTCACTGGAAGCAAATCGTAGTTCTTAC
AATGCATCCTTTCCCTCAAGCTTTGAACTCTCAGCAAGTTCCCACTCGGATGATGACGTC
ATCATAGCCAAAGAGGGAACTAGCGTTTCAATTGAGTGTCTTCTCACAGCCAGTCACTAT
GAAGATGTCCATTGGCACAATTCAAAAGGACAGCAACTGGATGGCAGAAGCAGAGGTGGA
AAGTGGTTGGTTTCTGATAACTTCCTAAACATCACCAATGTAGCTTTTGATGACCGTGGG
CTCTATACCTGTTTCGTCACCTCTCCAATTCGTGCCTCCTACTCTGTCACCCTACGTGTT
ATCTTCACCTCGGGAGACATGAGTGTCTATTACATGATTGTTTGCCTGATTGCCTTTACA
ATCACACTCATCTTGAATGTCACACGGCTGTGCATGATGAGCAGCCATCTTCGCAAGACT
GAGAAGGCCATCAATGAGTTCTTTAGAACTGAAGGGGCTGAGAAACTTCAGAAGGCCTTT
GAGATTGCAAAACGTATCCCCATCATTACCTCAGCCAAAACTCTGGAGCTCGCCAAAGTC
ACACAATTTAAGACCATGGAGTTTGCTCGTTATATTGAAGAACTGGCAAGAAGTGTCCCT
CTTCCACCTCTTATTCTAAACTGTCGAGCCTTTGTTGAGGAGATGTTTGAGGCTGTGCGA
GTGGATGACCCTGATGACCTGGGTGAAAGAATTAAAGAGAGACCTGCCTTGAATGCTCAA
GGTGGCATCTATGTCATTAACCCAGAGATGGGACGGAGTAATTCACCAGGAGGAGATTCA
GATGATGGCTCTCTGAATGAACAAGGCCAGGAAATAGCAGTTCAGGTTTCTGTCCACCTT
CAGTCAGAAACCAAAAGTATTGATACAGAGTCTCAAGGCAGCAGTCATTTCAGTCCACCT
GATGATATAGGATCTGCAGAATCTAACTGTAACTACAAAGATGGGGCATATGAAAACTGT
CAGCTG

ORF Protein

Cloned ORF protein sequence for pFN21AB4707 Download

>KIBB4707  362 aa
MKLHCCLFTLVASIIVPAAFVLEDVDFDQMVSLEANRSSYNASFPSSFELSASSHSDDDV
IIAKEGTSVSIECLLTASHYEDVHWHNSKGQQLDGRSRGGKWLVSDNFLNITNVAFDDRG
LYTCFVTSPIRASYSVTLRVIFTSGDMSVYYMIVCLIAFTITLILNVTRLCMMSSHLRKT
EKAINEFFRTEGAEKLQKAFEIAKRIPIITSAKTLELAKVTQFKTMEFARYIEELARSVP
LPPLILNCRAFVEEMFEAVRVDDPDDLGERIKERPALNAQGGIYVINPEMGRSNSPGGDS
DDGSLNEQGQEIAVQVSVHLQSETKSIDTESQGSSHFSPPDDIGSAESNCNYKDGAYENC
QL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4707 Download

>pFN21AB4707  5791 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGCTACATTGTTGCTTATTCACTTTAGTGGC
AAGTATTATTGTGCCAGCTGCTTTTGTTTTGGAAGATGTGGACTTCGACCAAATGGTTTC
ACTGGAAGCAAATCGTAGTTCTTACAATGCATCCTTTCCCTCAAGCTTTGAACTCTCAGC
AAGTTCCCACTCGGATGATGACGTCATCATAGCCAAAGAGGGAACTAGCGTTTCAATTGA
GTGTCTTCTCACAGCCAGTCACTATGAAGATGTCCATTGGCACAATTCAAAAGGACAGCA
ACTGGATGGCAGAAGCAGAGGTGGAAAGTGGTTGGTTTCTGATAACTTCCTAAACATCAC
CAATGTAGCTTTTGATGACCGTGGGCTCTATACCTGTTTCGTCACCTCTCCAATTCGTGC
CTCCTACTCTGTCACCCTACGTGTTATCTTCACCTCGGGAGACATGAGTGTCTATTACAT
GATTGTTTGCCTGATTGCCTTTACAATCACACTCATCTTGAATGTCACACGGCTGTGCAT
GATGAGCAGCCATCTTCGCAAGACTGAGAAGGCCATCAATGAGTTCTTTAGAACTGAAGG
GGCTGAGAAACTTCAGAAGGCCTTTGAGATTGCAAAACGTATCCCCATCATTACCTCAGC
CAAAACTCTGGAGCTCGCCAAAGTCACACAATTTAAGACCATGGAGTTTGCTCGTTATAT
TGAAGAACTGGCAAGAAGTGTCCCTCTTCCACCTCTTATTCTAAACTGTCGAGCCTTTGT
TGAGGAGATGTTTGAGGCTGTGCGAGTGGATGACCCTGATGACCTGGGTGAAAGAATTAA
AGAGAGACCTGCCTTGAATGCTCAAGGTGGCATCTATGTCATTAACCCAGAGATGGGACG
GAGTAATTCACCAGGAGGAGATTCAGATGATGGCTCTCTGAATGAACAAGGCCAGGAAAT
AGCAGTTCAGGTTTCTGTCCACCTTCAGTCAGAAACCAAAAGTATTGATACAGAGTCTCA
AGGCAGCAGTCATTTCAGTCCACCTGATGATATAGGATCTGCAGAATCTAACTGTAACTA
CAAAGATGGGGCATATGAAAACTGTCAGCTGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT