(Circular) (Six-base) MAPSORT of: pFN21AB4707.seq Check: 4554 from: 1 to: 5791 pFN21AB4707 5791 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2184 3601 278 Size: 53 83 186 936 648 1417 2468 Fragments arranged by size: 2468 1417 936 648 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 2595 2948 3854 5238 5506 1632 Size: 944 19 353 906 1384 268 1917 Fragments arranged by size: 1917 1384 944 906 353 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 2419 4690 5667 1203 Size: 1216 2271 977 1327 Fragments arranged by size: 2271 1327 1216 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 2033 2512 1166 Size: 867 479 4445 Fragments arranged by size: 4445 867 479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2281 3534 5106 2281 Size: 1253 1572 2966 Fragments arranged by size: 2966 1572 1253 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2434 3116 1870 Size: 73 491 682 4545 Fragments arranged by size: 4545 682 491 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3112 4567 618 Size: 325 217 627 1325 1455 1842 Fragments arranged by size: 1842 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 2367 2655 3110 1391 Size: 976 288 455 4072 Fragments arranged by size: 4072 976 455 288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 928 Size: 971 317 4503 Fragments arranged by size: 4503 971 317 BbvCI CC'TCA_GC Cuts at: 2635 2635 Size: 5791 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2630 3683 4893 2630 Size: 1053 1210 3528 Fragments arranged by size: 3528 1210 1053 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BfrBI ATG'CAT Cuts at: 2129 2129 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 3852 5008 1790 Size: 2062 1156 2573 Fragments arranged by size: 2573 2062 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3522 4480 442 Size: 416 53 83 399 24 2105 958 1753 Fragments arranged by size: 2105 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 4451 1239 Size: 1430 1782 2579 Fragments arranged by size: 2579 1782 1430 Bpu10I CC'TnA_GC Cuts at: 2635 2635 Size: 5791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2125 2826 2982 3916 4781 5079 2125 Size: 701 156 934 865 298 2837 Fragments arranged by size: 2837 934 865 701 298 156 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2818 4454 882 Size: 617 1319 1636 2219 Fragments arranged by size: 2219 1636 1319 617 BsaAI yAC'GTr Cuts at: 493 1204 2420 3582 493 Size: 711 1216 1162 2702 Fragments arranged by size: 2702 1216 1162 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 3598 3980 4615 Size: 53 83 186 936 648 1417 382 635 Cuts at: 4615 275 Size: 1451 Fragments arranged by size: 1451 1417 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5566 Fragments arranged by size: 5566 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 2910 1186 Size: 1591 133 4067 Fragments arranged by size: 4067 1591 133 BseYI C'CCAG_C Cuts at: 4994 5490 4994 Size: 496 5295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2655 3852 3937 5008 2655 Size: 1197 85 1071 3438 Fragments arranged by size: 3438 1197 1071 85 BsmI GAATG_Cn' Cuts at: 2842 3302 3395 2842 Size: 460 93 5238 Fragments arranged by size: 5238 460 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2655 3110 3852 3937 5008 1391 Size: 399 577 288 455 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 455 399 288 85 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2836 3146 844 Size: 1992 310 3489 Fragments arranged by size: 3489 1992 310 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4435 4622 1700 Size: 110 2625 187 2869 Fragments arranged by size: 2869 2625 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2647 2647 Size: 5791 BstYI r'GATC_y Cuts at: 1972 3035 3121 3488 3873 3890 5342 5428 5440 Size: 1063 86 367 385 17 1452 86 12 Cuts at: 5440 5786 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1063 385 367 346 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2359 2679 513 Size: 552 1294 320 3625 Fragments arranged by size: 3625 1294 552 320 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2447 4798 809 Size: 507 1131 2351 1802 Fragments arranged by size: 2351 1802 1131 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3238 4127 5756 Size: 54 1425 50 275 18 1408 889 1629 Cuts at: 5756 8 Size: 43 Fragments arranged by size: 1629 1425 1408 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 3721 1353 Size: 1377 991 3423 Fragments arranged by size: 3423 1377 991 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 2653 Size: 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2595 2669 2948 3854 4451 5238 Size: 393 944 19 74 279 906 597 787 Cuts at: 5238 5506 1239 Size: 268 1524 Fragments arranged by size: 1524 944 906 787 597 393 279 268 74 19 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 4938 1678 Size: 3260 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2897 2929 2978 Size: 32 135 32 943 32 350 32 49 Cuts at: 2978 3010 3020 3052 4432 4464 4506 4538 1373 Size: 32 10 32 1380 32 42 32 2626 Fragments arranged by size: 2626 1380 943 350 135 49 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 2143 748 Size: 1395 4396 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 2214 3390 2214 Size: 1176 4615 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3969 Fragments arranged by size: 3969 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2172 2512 3749 4108 4267 518 Size: 648 867 139 340 1237 359 159 2042 Fragments arranged by size: 2042 1237 867 648 359 340 159 139 MspA1I CmG'CkG Cuts at: 1606 2057 3088 3207 3884 5032 5277 1606 Size: 451 1031 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 677 451 245 119 NcoI C'CATG_G Cuts at: 513 1065 2679 513 Size: 552 1614 3625 Fragments arranged by size: 3625 1614 552 NdeI CA'TA_TG Cuts at: 387 3073 387 Size: 2686 3105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NsiI A_TGCA'T Cuts at: 2131 2131 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 3149 4694 1132 Size: 97 1920 1545 2229 Fragments arranged by size: 2229 1920 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5791 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 3043 3143 830 Size: 2213 100 3478 Fragments arranged by size: 3478 2213 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2057 3088 2057 Size: 1031 4760 SacI G_AGCT'C Cuts at: 2655 2655 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 3039 3139 4274 4955 5146 826 Size: 219 1994 100 1135 681 191 1471 Fragments arranged by size: 1994 1471 1135 681 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2140 2841 2997 3895 4796 5058 Size: 197 926 197 701 156 898 901 262 Cuts at: 5058 820 Size: 1553 Fragments arranged by size: 1553 926 901 898 701 262 197 197 156 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2600 1517 Size: 1083 4708 StyI C'CwwG_G Cuts at: 513 1065 2679 3230 513 Size: 552 1614 551 3074 Fragments arranged by size: 3074 1614 552 551 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 3818 3835 3988 4173 4618 325 Size: 1255 1215 1023 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1215 1023 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2182 3599 276 Size: 53 83 186 936 648 1417 2468 Fragments arranged by size: 2468 1417 936 648 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI