Restriction map for
pFN21AB4707
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# NO CUT ENZYMES ( 164 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AcsI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NgoMIV
NheI NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 175 GACGTC GACGT^C
AcyI 175 GACGTC G[GA]^CG[CT]C
AflIII 414 ACGTGT A^C[GA][CT]GT
AlwI 1030 GGATC GGATC(4/5)
AlwNI 271 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 646 GAGCTC G[AT]GC[AT]^C
AvaI 429 CTCGGG C^[CT]CG[GA]G
BbsI 218 GTCTTC GAAGAC(2/6)
BbvCI 629 CCTCAGC CCTCAGC(-5/-2)
BciVI 614 GTATCC GTATCC(6/5)
BfaI 200 CTAG C^TAG
BpmI 643 CTGGAG CTGGAG(16/14)
BpuAI 218 GTCTTC GAAGAC(2/6)
BsaAI 413 TACGTG [CT]AC^GT[GA]
BsaHI 175 GACGTC G[GA]^CG[CT]C
BsaI 819 GAGACC GGTCTC(1/5)
BsiHKAI 646 GAGCTC G[AT]GC[AT]^C
BsmI 831 GAATGC GAATGC(1/-1)
BsoBI 429 CTCGGG C^[CT]CG[GA]G
BspMI 822 ACCTGC ACCTGC(4/8)
BstXI 635 CCAAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1030 GGATCT [GA]^GATC[CT]
DpnII 1031 GATC ^GATC
DrdI 436 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 719 CTCTTC CTCTTC(1/4)
EcoT14I 674 CCATGG C^C[AT][AT]GG
EcoT22I 122 ATGCAT ATGCA^T
Hin1I 175 GACGTC G[GA]^CG[CT]C
HindIII 138 AAGCTT A^AGCTT
Ksp632I 719 CTCTTC CTCTTC(1/4)
MaeI 200 CTAG C^TAG
MboI 1031 GATC ^GATC
MfeI 209 CAATTG C^AATTG
MflI 1030 GGATCT [GA]^GATC[CT]
MunI 209 CAATTG C^AATTG
NcoI 674 CCATGG C^CATGG
NdeI 1067 CATATG CA^TATG
NdeII 1031 GATC ^GATC
NsiI 122 ATGCAT ATGCA^T
PleI 988 GAGTC GAGTC(4/5)
PstI 1034 CTGCAG CTGCA^G
SacI 646 GAGCTC GAGCT^C
Sau3AI 1031 GATC ^GATC
SfcI 1034 CTGCAG C^T[GA][CT]AG
StuI 593 AGGCCT AGG^CCT
StyI 674 CCATGG C^C[AT][AT]GG
TspRI 95 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 1030 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 358 GGGCTC G[GA]GC[CT]^C
646 GAGCTC
BmyI 358 GGGCTC G[AGT]GC[ACT]^C
646 GAGCTC
BseRI 757 GAGGAG GAGGAG(10/8)
890 GAGGAG
BsiYI 861 CCCAGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
862 CCAGAGATGGG
BslI 861 CCCAGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
862 CCAGAGATGGG
BsmFI 870 GGGAC GGGAC(10/14)
715 GTCCC
Bsp1286I 358 GGGCTC G[AGT]GC[ACT]^C
646 GAGCTC
Cac8I 48 GCCAGC GC[ACGT]^[ACGT]GC
648 GCTCGC
DsaI 354 CCGTGG C^C[GA][CT]GG
674 CCATGG
MspA1I 50 CAGCTG C[AC]G^C[GT]G
1081 CAGCTG
PvuII 50 CAGCTG CAG^CTG
1081 CAGCTG
SfaNI 124 GCATC GCATC(5/9)
845 GCATC
TaqI 80 TCGA T^CGA
744 TCGA
TfiI 895 GATTC G^A[AT]TC
1039 GAATC
TthHB8I 80 TCGA T^CGA
744 TCGA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BsaJI BsmAI BsrI
BssKI BstF5I BstNI DdeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIII
ScrFI SmlI Tru9I TseI Tsp45I
Tsp509I