KRT20 ORF clone FHC10267

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10267

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Clone info.

Species

human

Clone Name

pFN21AB6659 Sequence (with vector) Restriction Map

Product ID

FHC10267

Flexi Clone PID

FXC10267

ORF Sequence

DNA sequence (1272 bp)
Protein sequence (424 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528550]

Gene

KIBB6659

Symbol

KRT20

Alias : CD20, CK-20, CK20, K20, KRT21

Description

keratin 20, type I

Original Clone ID

cs01259

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1272 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 424 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11379.1 2.9e-82 100.0 KRT20 CCDS11402.1 6.6e-38 52.7 KRT17 CCDS11400.1 1.2e-37 53.8 KRT14 CCDS11398.1 8e-35 50.1 KRT15 CCDS11401.1 8.2e-35 49.9 KRT16 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 148 161 PR01248 Keratin IPR002957 169 192 PR01248 Keratin IPR002957 223 243 PR01248 Keratin IPR002957 295 310 PR01248 Keratin IPR002957 321 347 PR01248 Keratin HMMPfam IPR016044 69 379 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061883 424 608218 100.0 100.0 NP_000413 432 148069,167210,184500 52.7 91.3 NP_005548 473 148067,167200,613000 49.9 90.1 NP_002266 456 148030 50.1 92.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6659 Download

>KIBB6659  1272 bp
ATGGATTTCAGTCGCAGAAGCTTCCACAGAAGCCTGAGCTCCTCCTTGCAGGCCCCTGTA
GTCAGTACAGTGGGCATGCAGCGCCTCGGGACGACACCCAGCGTTTATGGGGGTGCTGGA
GGCCGGGGCATCCGCATCTCCAACTCCAGACACACGGTGAACTATGGGAGCGATCTCACA
GGCGGCGGGGACCTGTTTGTTGGCAATGAGAAAATGGCCATGCAGAACCTAAATGACCGT
CTAGCGAGCTACCTAGAAAAGGTGCGGACCCTGGAGCAGTCCAACTCCAAACTTGAAGTG
CAAATCAAGCAGTGGTACGAAACCAACGCCCCGAGGGCTGGTCGCGACTACAGTGCATAT
TACAGACAAATTGAAGAGCTGCGAAGTCAGATTAAGGATGCTCAACTGCAAAATGCTCGG
TGTGTCCTGCAAATTGATAATGCTAAACTGGCTGCTGAGGACTTCAGACTGAAGTATGAG
ACTGAGAGAGGAATACGTCTAACAGTGGAAGCTGATCTCCAAGGCCTGAATAAGGTCTTT
GATGACCTAACCCTACATAAAACAGATTTGGAGATTCAAATTGAAGAACTGAATAAAGAC
CTAGCTCTCCTCAAAAAGGAGCATCAGGAGGAAGTCGATGGCCTACACAAGCATCTGGGC
AACACTGTCAATGTGGAGGTTGATGCTGCTCCAGGCCTGAACCTTGGCGTCATCATGAAT
GAAATGAGGCAGAAGTATGAAGTCATGGCCCAGAAGAACCTTCAAGAGGCCAAAGAACAG
TTTGAGAGACAGACTGCAGTTCTGCAGCAACAGGTCACAGTGAATACTGAAGAATTAAAA
GGAACTGAGGTTCAACTAACGGAGCTGAGACGCACCTCCCAGAGCCTTGAGATAGAACTC
CAGTCCCATCTCAGCATGAAAGAGTCTTTGGAGCACACTCTAGAGGAGACCAAGGCCCGT
TACAGCAGCCAGTTAGCCAACCTCCAGTCGCTGTTGAGCTCTCTGGAGGCCCAACTGATG
CAGATTCGGAGTAACATGGAACGCCAGAACAACGAATACCATATCCTTCTTGACATAAAG
ACTCGACTTGAACAGGAAATTGCTACTTACCGCCGCCTTCTGGAAGGAGAAGACGTAAAA
ACTACAGAATATCAGTTAAGCACCCTGGAAGAGAGAGATATAAAGAAAACCAGGAAGATT
AAGACAGTCGTGCAAGAAGTAGTGGATGGCAAGGTCGTGTCATCTGAAGTCAAAGAGGTG
GAAGAAAATATC

ORF Protein

Cloned ORF protein sequence for pFN21AB6659 Download

>KIBB6659  424 aa
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLT
GGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAY
YRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVF
DDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMN
EMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIEL
QSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIK
TRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEV
EENI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6659 Download

>pFN21AB6659  5977 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATTTCAGTCGCAGAAGCTTCCACAGAAGCCT
GAGCTCCTCCTTGCAGGCCCCTGTAGTCAGTACAGTGGGCATGCAGCGCCTCGGGACGAC
ACCCAGCGTTTATGGGGGTGCTGGAGGCCGGGGCATCCGCATCTCCAACTCCAGACACAC
GGTGAACTATGGGAGCGATCTCACAGGCGGCGGGGACCTGTTTGTTGGCAATGAGAAAAT
GGCCATGCAGAACCTAAATGACCGTCTAGCGAGCTACCTAGAAAAGGTGCGGACCCTGGA
GCAGTCCAACTCCAAACTTGAAGTGCAAATCAAGCAGTGGTACGAAACCAACGCCCCGAG
GGCTGGTCGCGACTACAGTGCATATTACAGACAAATTGAAGAGCTGCGAAGTCAGATTAA
GGATGCTCAACTGCAAAATGCTCGGTGTGTCCTGCAAATTGATAATGCTAAACTGGCTGC
TGAGGACTTCAGACTGAAGTATGAGACTGAGAGAGGAATACGTCTAACAGTGGAAGCTGA
TCTCCAAGGCCTGAATAAGGTCTTTGATGACCTAACCCTACATAAAACAGATTTGGAGAT
TCAAATTGAAGAACTGAATAAAGACCTAGCTCTCCTCAAAAAGGAGCATCAGGAGGAAGT
CGATGGCCTACACAAGCATCTGGGCAACACTGTCAATGTGGAGGTTGATGCTGCTCCAGG
CCTGAACCTTGGCGTCATCATGAATGAAATGAGGCAGAAGTATGAAGTCATGGCCCAGAA
GAACCTTCAAGAGGCCAAAGAACAGTTTGAGAGACAGACTGCAGTTCTGCAGCAACAGGT
CACAGTGAATACTGAAGAATTAAAAGGAACTGAGGTTCAACTAACGGAGCTGAGACGCAC
CTCCCAGAGCCTTGAGATAGAACTCCAGTCCCATCTCAGCATGAAAGAGTCTTTGGAGCA
CACTCTAGAGGAGACCAAGGCCCGTTACAGCAGCCAGTTAGCCAACCTCCAGTCGCTGTT
GAGCTCTCTGGAGGCCCAACTGATGCAGATTCGGAGTAACATGGAACGCCAGAACAACGA
ATACCATATCCTTCTTGACATAAAGACTCGACTTGAACAGGAAATTGCTACTTACCGCCG
CCTTCTGGAAGGAGAAGACGTAAAAACTACAGAATATCAGTTAAGCACCCTGGAAGAGAG
AGATATAAAGAAAACCAGGAAGATTAAGACAGTCGTGCAAGAAGTAGTGGATGGCAAGGT
CGTGTCATCTGAAGTCAAAGAGGTGGAAGAAAATATCGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT