(Circular) (Six-base) MAPSORT of: pFN21AB6659.seq Check: 7565 from: 1 to: 5977 pFN21AB6659 5977 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2452 2495 2853 3270 4040 5424 5692 1632 Size: 820 43 358 417 770 1384 268 1917 Fragments arranged by size: 1917 1384 820 770 417 358 268 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2838 2870 2838 Size: 32 5945 AlwNI CAG_nnn'CTG Cuts at: 2995 3720 5292 2995 Size: 725 1572 3680 Fragments arranged by size: 3680 1572 725 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2335 3302 1870 Size: 73 147 245 967 4545 Fragments arranged by size: 4545 967 245 147 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2045 3005 3296 1391 Size: 654 960 291 4072 Fragments arranged by size: 4072 960 654 291 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3141 928 Size: 971 1242 3764 Fragments arranged by size: 3764 1242 971 BbvCI CC'TCA_GC Cuts at: 2460 2460 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2339 2373 2402 2436 4168 4202 1903 Size: 34 402 34 29 34 1732 34 3678 Fragments arranged by size: 3678 1732 402 34 34 34 34 29 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2339 4588 136 Size: 107 122 71 631 605 667 2249 1525 Fragments arranged by size: 2249 1525 667 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 4038 5194 1790 Size: 2248 1156 2573 Fragments arranged by size: 2573 2248 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2142 2297 2679 2888 2972 3029 4637 Size: 895 8 155 382 209 84 57 1608 Cuts at: 4637 1239 Size: 2579 Fragments arranged by size: 2579 1608 895 382 209 155 84 57 8 Bpu10I CC'TnA_GC Cuts at: 2039 2460 2039 Size: 421 5556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2912 4102 4967 5265 2912 Size: 1190 865 298 3624 Fragments arranged by size: 3624 1190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2945 4640 882 Size: 617 1446 1695 2219 Fragments arranged by size: 2219 1695 1446 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2712 3784 4166 4801 Size: 53 83 186 936 1179 1072 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1451 1179 1072 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3123 3153 1178 Size: 30 165 30 1720 30 4002 Fragments arranged by size: 4002 1720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2603 2963 1186 Size: 849 568 360 4200 Fragments arranged by size: 4200 849 568 360 BseYI C'CCAG_C Cuts at: 2102 5180 5676 2102 Size: 3078 496 2403 Fragments arranged by size: 3078 2403 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 2940 3005 4038 4123 5194 2045 Size: 895 65 1033 85 1071 2828 Fragments arranged by size: 2828 1071 1033 895 85 65 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2866 2866 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2940 3005 3296 4038 4123 5194 Size: 399 255 895 65 291 742 85 1071 Cuts at: 5194 1391 Size: 2174 Fragments arranged by size: 2174 1071 895 742 399 291 255 85 65 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2718 3866 5596 2718 Size: 1148 1730 3099 Fragments arranged by size: 3099 1730 1148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2215 4472 4646 65 Size: 1183 967 2257 174 1396 Fragments arranged by size: 2257 1396 1183 967 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972 Size: 1335 367 385 17 1452 86 12 346 Cuts at: 5972 1972 Size: 1977 Fragments arranged by size: 1977 1452 1335 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2321 3493 4287 4307 2321 Size: 1172 794 20 3991 Fragments arranged by size: 3991 1172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2161 2161 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 3424 4313 Size: 54 1425 50 275 18 390 1204 889 Cuts at: 4313 5942 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1204 889 390 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 3168 3907 1353 Size: 1020 795 739 3423 Fragments arranged by size: 3423 1020 795 739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2043 3003 2043 Size: 960 5017 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2142 2297 2452 2495 2679 2853 Size: 393 502 8 155 155 43 184 174 Cuts at: 2853 2888 2972 3029 3270 4040 4637 5424 5692 Size: 35 84 57 241 770 597 787 268 Cuts at: 5692 1239 Size: 1524 Fragments arranged by size: 1524 787 770 597 502 393 268 241 184 174 155 155 84 57 43 35 8 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2534 2618 2768 2261 Size: 273 84 150 5470 Fragments arranged by size: 5470 273 150 84 EcoO109I rG'GnC_Cy Cuts at: 2056 2194 2056 Size: 138 5839 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2089 5124 1678 Size: 411 3035 2531 Fragments arranged by size: 3035 2531 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2329 2361 4618 4650 4692 Size: 32 135 32 757 32 2257 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 2257 757 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 2023 748 Size: 1275 4702 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2310 5091 5275 1305 Size: 864 141 2781 184 2007 Fragments arranged by size: 2781 2007 864 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 10 Size: 54 1425 343 390 3765 Fragments arranged by size: 3765 1425 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3935 4294 4453 518 Size: 648 2769 359 159 2042 Fragments arranged by size: 2769 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3393 4070 5218 5463 1606 Size: 1787 677 1148 245 2120 Fragments arranged by size: 2120 1787 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NruI TCG'CGA Cuts at: 2349 2349 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2083 3335 4880 1132 Size: 97 854 1252 1545 2229 Fragments arranged by size: 2229 1545 1252 854 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2194 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3044 3076 3044 Size: 32 5945 PstI C_TGCA'G Cuts at: 830 2803 2811 3329 830 Size: 1973 8 518 3478 Fragments arranged by size: 3478 1973 518 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 SacI G_AGCT'C Cuts at: 2045 3005 2045 Size: 960 5017 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2373 2373 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2061 2353 2799 2807 3147 3325 4460 Size: 219 1016 292 446 8 340 178 1135 Cuts at: 4460 5141 5332 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1016 681 446 340 292 219 191 178 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2891 4081 4982 5244 820 Size: 197 926 948 1190 901 262 1553 Fragments arranged by size: 1553 1190 948 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 2083 3335 1132 Size: 951 1252 3774 Fragments arranged by size: 3774 1252 951 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2529 2700 1517 Size: 1012 171 4794 Fragments arranged by size: 4794 1012 171 StyI C'CwwG_G Cuts at: 513 1065 2004 2524 2707 2955 3416 513 Size: 552 939 520 183 248 461 3074 Fragments arranged by size: 3074 939 552 520 461 248 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2116 4004 4021 4174 4359 4804 325 Size: 1255 536 1888 17 153 185 445 1498 Fragments arranged by size: 1888 1498 1255 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 4223 5935 Size: 274 80 33 51 1443 92 2154 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 2154 1712 1443 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 2944 3313 2944 Size: 369 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI