RTKN ORF clone FHC08910

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08910

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Clone info.

Species

human

Clone Name

pFN21AB5288 Sequence (with vector) Restriction Map

Product ID

FHC08910

Flexi Clone PID

FXC08910

Original Clone PID

ORK08910

ORF Sequence

DNA sequence (1650 bp)
Protein sequence (550 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527260]

Gene

KIBB5288

Symbol

RTKN

Description

rhotekin, transcript variant 2

Original Clone ID

bm03069

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1650 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 550 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1941.1 5.5e-161 100.0 RTKN CCDS33226.1 1.7e-154 100.0 RTKN CCDS42699.1 1.7e-150 100.0 RTKN CCDS7263.1 2.2e-52 40.7 RTKN2 CCDS73140.1 2.2e-14 45.5 RTKN2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000861 26 84 PF02185 HR1 repeat IPR001849 300 400 PF00169 Pleckstrin homology domain HMMSmart IPR011072 23 86 SM00742 HR1 rho-binding repeat IPR001849 297 405 SM00233 Pleckstrin homology domain ProfileScan IPR001849 296 403 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_149035 550 602288 100.0 100.0 XP_016860124 550 602288 100.0 100.0 NP_001015055 563 602288 100.0 95.8 NP_001015056 513 602288 100.0 93.3 XP_016860125 477 602288 93.5 86.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5288 Download

>KIBB5288  1650 bp
ATGCAGGACAGATTGCACATCCTGGAGGACCTGAATATGCTCTACATTCGGCAGATGGCA
CTCAGCCTGGAGGACACGGAGTTGCAGAGGAAGCTAGACCATGAGATCCGGATGAGGGAA
GGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAGGCTCTGGAGGCCACCAAG
AGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGCGAGCTGCAGCGGCGCAAG
GAGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCCTTCTGACAGTGGCCCGCCCGCTGAG
CGCTCCCCCTGCCGCGGCCGGGTCTGCATCTCTGACCTCCGGATTCCACTCATGTGGAAG
GACACAGAATATTTCAAGAACAAAGGTGACTTGCACCGCTGGGCTGTGTTCCTGCTGCTG
CAGCTGGGGGAACACATCCAGGACACAGAGATGATCCTAGTGGACAGGACCCTCACAGAC
ATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAGGCGGGGCCAGACTTTGAACTGCGGTTA
GAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGCCCTGACTGGCGGCCCCAAGAGGCTT
GCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGGCGTGTCCGGGCATCGCTG
GACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCCACCCCAGTTGTTGGTGGT
CCTCGTTACCACCTCTTGGCTCACACCACACTCACCCTGGCAGCAGTGCAAGATGGATTC
CGCACACATGACCTCACCCTTGCCAGTCATGAGGAGAACCCTGCCTGGCTGCCCCTTTAT
GGTAGCGTGTGTTGCCGTCTGGCAGCTCAGCCTCTCTGCATGACTCAGCCCACTGCAAGT
GGTACCCTCAGGGTGCAGCAAGCTGGGGAGATGCAGAACTGGGCACAAGTGCATGGAGTT
CTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCTGAGGATGCAGACACTGGGGAAGAG
CCGCTGCTTACTATTGCTGTCAACAAGGAGACTCGAGTCCGGGCAGGGGAGCTGGACCAG
GCTCTAGGACGGCCCTTCACCCTAAGCATCAGTAACCAGTATGGGGATGATGAGGTGACA
CACACCCTTCAGACAGAAAGTCGGGAAGCACTGCAGAGCTGGATGGAGGCTCTGTGGCAG
CTTTTCTTTGACATGAGCCAATGGAAGCAGTGCTGTGATGAAATCATGAAAATTGAAACT
CCTGCTCCCCGGAAACCACCCCAAGCACTGGCAAAGCAGGGGTCCTTGTACCATGAGATG
GCTATTGAGCCGCTGGATGACATCGCAGCGGTGACAGACATCCTGACCCAGCGGGAGGGC
GCAAGGCTGGAGACACCCCCACCCTGGCTGGCAATGTTTACAGACCAGCCTGCCCTGCCT
AACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCCAGACTGGACCCACCCCCTGCCCTGG
GGGAGACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGACCACTCCCCTAGGGCTCGC
TCGGTTGCCCCCCTCCCACCTCAGCGATCCCCACGGACCAGAGGCCTCTGCAGCAAAGGC
CAACCTCGCACTTGGCTCCAGTCACCAGTG

ORF Protein

Cloned ORF protein sequence for pFN21AB5288 Download

>KIBB5288  550 aa
MQDRLHILEDLNMLYIRQMALSLEDTELQRKLDHEIRMREGACKLLAACSQREQALEATK
SLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRGRVCISDLRIPLMWK
DTEYFKNKGDLHRWAVFLLLQLGEHIQDTEMILVDRTLTDISFQSNVLFAEAGPDFELRL
ELYGACVEEEGALTGGPKRLATKLSSSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGG
PRYHLLAHTTLTLAAVQDGFRTHDLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTAS
GTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAGELDQ
ALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMSQWKQCCDEIMKIET
PAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALP
NPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKG
QPRTWLQSPV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5288 Download

>pFN21AB5288  6355 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGGACAGATTGCACATCCTGGAGGACCTGAA
TATGCTCTACATTCGGCAGATGGCACTCAGCCTGGAGGACACGGAGTTGCAGAGGAAGCT
AGACCATGAGATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCG
AGAGCAGGCTCTGGAGGCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTA
CATGGGCGAGCTGCAGCGGCGCAAGGAGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCC
TTCTGACAGTGGCCCGCCCGCTGAGCGCTCCCCCTGCCGCGGCCGGGTCTGCATCTCTGA
CCTCCGGATTCCACTCATGTGGAAGGACACAGAATATTTCAAGAACAAAGGTGACTTGCA
CCGCTGGGCTGTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGACACAGAGATGAT
CCTAGTGGACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAGGC
GGGGCCAGACTTTGAACTGCGGTTAGAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGC
CCTGACTGGCGGCCCCAAGAGGCTTGCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTC
AGGGAGGCGTGTCCGGGCATCGCTGGACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTT
GCTCCCCACCCCAGTTGTTGGTGGTCCTCGTTACCACCTCTTGGCTCACACCACACTCAC
CCTGGCAGCAGTGCAAGATGGATTCCGCACACATGACCTCACCCTTGCCAGTCATGAGGA
GAACCCTGCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGCCGTCTGGCAGCTCAGCCTCT
CTGCATGACTCAGCCCACTGCAAGTGGTACCCTCAGGGTGCAGCAAGCTGGGGAGATGCA
GAACTGGGCACAAGTGCATGGAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACC
TGAGGATGCAGACACTGGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCG
AGTCCGGGCAGGGGAGCTGGACCAGGCTCTAGGACGGCCCTTCACCCTAAGCATCAGTAA
CCAGTATGGGGATGATGAGGTGACACACACCCTTCAGACAGAAAGTCGGGAAGCACTGCA
GAGCTGGATGGAGGCTCTGTGGCAGCTTTTCTTTGACATGAGCCAATGGAAGCAGTGCTG
TGATGAAATCATGAAAATTGAAACTCCTGCTCCCCGGAAACCACCCCAAGCACTGGCAAA
GCAGGGGTCCTTGTACCATGAGATGGCTATTGAGCCGCTGGATGACATCGCAGCGGTGAC
AGACATCCTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCTGGCTGGCAAT
GTTTACAGACCAGCCTGCCCTGCCTAACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCC
AGACTGGACCCACCCCCTGCCCTGGGGGAGACCCCGAACCTTTTCCCTGGATGCTGTCCC
CCCAGACCACTCCCCTAGGGCTCGCTCGGTTGCCCCCCTCCCACCTCAGCGATCCCCACG
GACCAGAGGCCTCTGCAGCAAAGGCCAACCTCGCACTTGGCTCCAGTCACCAGTGGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT