(Circular) (Six-base) MAPSORT of: pFN21AB5288.seq Check: 4836 from: 1 to: 6355 pFN21AB5288 6355 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2242 2242 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3698 1937 Size: 1761 4594 Acc65I G'GTAC_C Cuts at: 2906 3676 2906 Size: 770 5585 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 4418 5802 6070 1632 Size: 1505 1281 1384 268 1917 Fragments arranged by size: 1917 1505 1384 1281 268 AfeI AGC'GCT Cuts at: 2306 2306 Size: 6355 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 3162 5084 3162 Size: 1922 4433 AleI CACnn'nnGTG Cuts at: 1166 2356 1166 Size: 1190 5165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 2170 2257 3014 4098 5670 2170 Size: 87 757 1084 1572 2855 Fragments arranged by size: 2855 1572 1084 757 87 ApaI G_GGCC'C Cuts at: 2581 2581 Size: 6355 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 3664 3890 4042 6151 1493 Size: 2171 226 152 2109 1697 Fragments arranged by size: 2171 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3057 3680 1870 Size: 73 1114 623 4545 Fragments arranged by size: 4545 1114 623 73 AvrII C'CTAG_G Cuts at: 3554 3554 Size: 6355 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2906 3676 5131 618 Size: 325 217 627 1119 770 1455 1842 Fragments arranged by size: 1842 1455 1119 770 627 325 217 BanII G_rGCy'C Cuts at: 1391 2581 3562 3674 1391 Size: 1190 981 112 4072 Fragments arranged by size: 4072 1190 981 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 5013 Fragments arranged by size: 5013 971 371 BbvCI CC'TCA_GC Cuts at: 2213 2514 3585 2213 Size: 301 1071 4983 Fragments arranged by size: 4983 1071 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4546 4580 1903 Size: 34 50 34 2525 34 3678 Fragments arranged by size: 3678 2525 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3467 4966 136 Size: 107 122 71 631 605 1795 1499 1525 Fragments arranged by size: 1795 1525 1499 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 2301 2871 3772 2301 Size: 570 901 4884 Fragments arranged by size: 4884 901 570 Bme1580I G_kGCm'C Cuts at: 1790 2581 2950 4416 5572 1790 Size: 791 369 1466 1156 2573 Fragments arranged by size: 2573 1466 1156 791 369 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2705 2953 3024 Size: 416 53 83 399 24 1288 248 71 Cuts at: 3024 4086 5044 442 Size: 1062 958 1753 Fragments arranged by size: 1753 1288 1062 958 416 399 248 83 71 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2093 2192 3413 3626 5015 1239 Size: 809 45 99 1221 213 1389 2579 Fragments arranged by size: 2579 1389 1221 809 213 99 45 Bpu10I CC'TnA_GC Cuts at: 2213 2514 3107 3585 2213 Size: 301 593 478 4983 Fragments arranged by size: 4983 593 478 301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3502 5018 882 Size: 617 2003 1516 2219 Fragments arranged by size: 2219 2003 1516 617 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4162 4544 5179 275 Size: 53 83 186 936 2629 382 635 1451 Fragments arranged by size: 2629 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 2343 4788 5186 5460 5607 6018 2112 Size: 231 2445 398 274 147 411 2449 Fragments arranged by size: 2449 2445 411 398 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3182 3212 3253 3283 1178 Size: 30 165 30 1779 30 41 30 4250 Fragments arranged by size: 4250 1779 165 41 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2626 2831 1186 Size: 1440 205 4710 Fragments arranged by size: 4710 1440 205 BseYI C'CCAG_C Cuts at: 2154 2257 2403 2428 2672 2927 3372 3472 5558 Size: 103 146 25 244 255 445 100 2086 Cuts at: 5558 6054 2154 Size: 496 2455 Fragments arranged by size: 2455 2086 496 445 255 244 146 103 100 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2939 1163 Size: 573 1203 4579 Fragments arranged by size: 4579 1203 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 3805 4566 5594 1540 Size: 275 186 322 1482 761 1028 2301 Fragments arranged by size: 2301 1482 1028 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 4416 4501 5572 2508 Size: 1908 85 1071 3291 Fragments arranged by size: 3291 1908 1071 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2581 2950 3562 3674 4416 4501 Size: 399 718 73 369 612 112 742 85 Cuts at: 4501 5572 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 718 612 399 369 112 85 73 BspEI T'CCGG_A Cuts at: 2112 2343 6018 2112 Size: 231 3675 2449 Fragments arranged by size: 3675 2449 231 BspHI T'CATG_A Cuts at: 2812 3249 4244 5974 2812 Size: 437 995 1730 3193 Fragments arranged by size: 3193 1730 995 437 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 3710 844 Size: 1398 1468 3489 Fragments arranged by size: 3489 1468 1398 BsrBI CCG'CTC Cuts at: 1394 1823 2634 4242 1394 Size: 429 811 1608 3507 Fragments arranged by size: 3507 1608 811 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 3423 4850 5024 65 Size: 1183 1258 917 1427 174 1396 Fragments arranged by size: 1427 1396 1258 1183 917 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2271 2991 4999 5186 1700 Size: 110 461 720 2008 187 2869 Fragments arranged by size: 2869 2008 720 461 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6355 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 2358 2358 Size: 6355 BstYI r'GATC_y Cuts at: 1972 2109 3685 4052 4437 4454 5906 5992 6004 Size: 137 1576 367 385 17 1452 86 12 Cuts at: 6004 6350 1972 Size: 346 1977 Fragments arranged by size: 1977 1576 1452 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2638 2912 3000 2638 Size: 274 88 5993 Fragments arranged by size: 5993 274 88 BtgI C'CryG_G Cuts at: 513 1065 2317 3596 513 Size: 552 1252 1279 3272 Fragments arranged by size: 3272 1279 1252 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2643 3331 487 Size: 683 56 1417 688 3511 Fragments arranged by size: 3511 1417 688 683 56 BtsI GCAGTG_nn' Cuts at: 2775 2895 3173 3239 3871 4665 4685 2775 Size: 120 278 66 632 794 20 4445 Fragments arranged by size: 4445 794 632 278 120 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2695 2730 529 Size: 35 2131 35 4154 Fragments arranged by size: 4154 2131 35 35 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 3802 4691 Size: 54 1425 50 275 18 490 1482 889 Cuts at: 4691 6320 8 Size: 1629 43 Fragments arranged by size: 1629 1482 1425 889 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2320 3802 1537 Size: 275 508 1482 4090 Fragments arranged by size: 4090 1482 508 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 2565 2987 3015 4285 1353 Size: 1160 52 422 28 1270 3423 Fragments arranged by size: 3423 1270 1160 422 52 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4942 5316 5462 387 Size: 1064 138 3353 374 146 1280 Fragments arranged by size: 3353 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2093 2192 3137 3413 3626 4418 Size: 393 416 45 99 945 276 213 792 Cuts at: 4418 5015 5802 6070 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 945 792 787 597 416 393 276 268 213 99 45 EcoNI CCTnn'n_nnAGG Cuts at: 2641 2641 Size: 6355 EcoO109I rG'GnC_Cy Cuts at: 2032 2127 2472 2556 2577 2578 3306 2032 Size: 95 345 84 21 1 728 5081 Fragments arranged by size: 5081 728 345 95 84 21 1 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 2308 5502 1678 Size: 630 3194 2531 Fragments arranged by size: 3194 2531 630 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2007 2039 3148 3178 3180 Size: 32 135 32 435 32 1109 30 2 Cuts at: 3180 3210 4996 5028 5070 5102 1373 Size: 30 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 1109 435 135 42 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3046 3699 3945 669 Size: 988 281 1108 653 246 3079 Fragments arranged by size: 3079 1108 988 653 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 2910 3680 2910 Size: 770 5585 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5469 5653 1305 Size: 4164 184 2007 Fragments arranged by size: 4164 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2356 2453 4313 4672 4831 518 Size: 648 1190 97 1860 359 159 2042 Fragments arranged by size: 2042 1860 1190 648 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2236 2300 2319 2403 2428 3028 3337 3353 Size: 630 64 19 84 25 600 309 16 Cuts at: 3353 3376 3771 4448 5596 5841 1606 Size: 23 395 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 600 395 309 245 84 64 25 23 19 16 NaeI GCC'GGC Cuts at: 2273 2273 Size: 6355 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5803 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NgoMIV G'CCGG_C Cuts at: 2271 2271 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 3713 5258 1132 Size: 97 2484 1545 2229 Fragments arranged by size: 2484 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2626 3501 1348 Size: 1278 875 4202 Fragments arranged by size: 4202 1278 875 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 2717 2740 3292 3322 1108 Size: 1609 23 552 30 4141 Fragments arranged by size: 4141 1609 552 30 23 PfoI T'CCnGG_A Cuts at: 2025 2442 2025 Size: 417 5938 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2472 3306 2032 Size: 440 834 5081 Fragments arranged by size: 5081 834 440 PshAI GACnn'nnGTC Cuts at: 1613 3059 1613 Size: 1446 4909 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PspOMI G'GGCC_C Cuts at: 2577 2577 Size: 6355 PspXI vC'TCGA_Gb Cuts at: 3057 3057 Size: 6355 PstI C_TGCA'G Cuts at: 830 2235 2427 3180 3617 3707 830 Size: 1405 192 753 437 90 3478 Fragments arranged by size: 3478 1405 753 437 192 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2428 2428 Size: 6355 SacII CC_GC'GG Cuts at: 2320 2320 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 3015 2513 Size: 502 5853 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2231 2423 3176 3613 3703 4838 5519 Size: 219 1186 192 753 437 90 1135 681 Cuts at: 5519 5710 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 753 681 437 219 192 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3057 4459 5360 5622 820 Size: 197 926 1114 1402 901 262 1553 Fragments arranged by size: 1553 1402 1114 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 2375 4279 5 Size: 47 2323 1904 2081 Fragments arranged by size: 2323 2081 1904 47 StuI AGG'CCT Cuts at: 1517 3609 1517 Size: 2092 4263 StyI C'CwwG_G Cuts at: 513 1065 3554 3794 513 Size: 552 2489 240 3074 Fragments arranged by size: 3074 2489 552 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4382 4399 4552 4737 5182 325 Size: 1255 2802 17 153 185 445 1498 Fragments arranged by size: 2802 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4601 6313 96 Size: 274 80 33 51 1443 2624 1712 138 Fragments arranged by size: 2624 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3182 3214 6315 6347 3182 Size: 32 3101 32 3190 Fragments arranged by size: 3190 3101 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 3480 2758 Size: 722 5633 XhoI C'TCGA_G Cuts at: 1943 3057 1943 Size: 1114 5241 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 3520 4484 3520 Size: 964 5391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI