APPL2 ORF clone FHC03605

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03605

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Clone info.

Species

human

Clone Name

pFN21AB6205 Sequence (with vector) Restriction Map

Product ID

FHC03605

Flexi Clone PID

FXC03605

ORF Sequence

DNA sequence (1992 bp)
Protein sequence (664 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527678]

Gene

KIBB6205

Symbol

APPL2

Alias : DIP13B

Description

adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2, transcript variant 1

Original Clone ID

cs00805

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1992 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 664 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9101.1 0 100.0 APPL2 CCDS58276.1 0 99.1 APPL2 CCDS58275.1 0 100.0 APPL2 CCDS2882.1 1.6e-122 55.0 APPL1 CCDS19.2 3.4e-17 24.2 ACAP3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 279 374 PF00169 Pleckstrin homology domain IPR006020 494 609 PF00640 Phosphotyrosine interaction domain HMMSmart IPR001849 278 377 SM00233 Pleckstrin homology domain IPR006020 489 622 SM00462 Phosphotyrosine interaction domain ProfileScan IPR001849 277 375 PS50003 Pleckstrin homology domain IPR006020 494 564 PS01179 Phosphotyrosine interaction domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060641 664 606231 100.0 100.0 NP_001238833 670 606231 99.1 100.0 XP_016875040 651 606231 99.8 97.7 XP_011536832 657 606231 98.9 97.7 XP_016875041 621 606231 100.0 93.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6205 Download

>KIBB6205  1992 bp
ATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGCGTTGCAGGACAGCCCCCAGACTCGC
TCTTTACTGAGCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATACCAACCAGCTG
CTCCAGGCAATGCAGCGCGTCTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAG
CTTTCTAAGCAACTGCTGGCATATGAAAAACAGAACTTTGCTCTTGGCAAAGGTGATGAA
GAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGGATGAGCTTAATCTTCTCCAT
ACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATACAATTCCGAGAA
AAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTAGCAATGAGCAT
GACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAGGTGAAG
ACCGAAGTCGGAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCACCTCTCCTCCCTTCAG
TACTACTGTGCCCTCAACGCGCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATG
ATAGGCTTTGCCCATGGACAGATTAACTTTTTTAAGAAGGGAGCAGAGATGTTTTCCAAA
CGTATGGACAGCTTTTTATCCTCCGTTGCAGACATGGTTCAAAGCATTCAGGTAGAACTG
GAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCAAGAATTACTTTCTGTTGATGAATCT
GTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATCAACAGGAACCTCATCCAGAAG
GCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACCTGGGAGAGGCTT
TATTTCTTCACCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGAGGT
TTGATCCAGGACCTGGACAACTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTAC
TGCTTCCAGATCACCACGCCCAATGGAAAATCGGGAATAATCCTCCAGGCTGAGAGCAGA
AAGGAAAATGAAGAGTGGATATGTGCAATAAACAACATCTCCAGACAGATCTACCTGACC
GACAACCCTGAGGCAGTCGCGATCAAGTTGAATCAGACCGCTCTGCAAGCAGTGACTCCC
ATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATGCCCCAGCCAGAACCTGAAAAATTCA
GAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGCAACAGCCAGTCTACCTGAAGCA
GAGGAGCTGATCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCTGCTACAGAA
TTCCTTGATCAGAACAGAGGGAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAA
TCATTTCCAGAAGCAGAAGATTCTCTTTTGCAGCAGATGTTTATAGTTCGGTTTTTGGGA
TCAATGGCAGTTAAAACAGACAGCACTACTGAAGTGATTTATGAAGCGATGAGACAAGTA
TTGGCTGCTCGGGCTATTCATAACATCTTCCGCATGACAGAATCCCATCTGATGGTCACC
AGTCAATCTTTGAGGTTGATAGATCCACAGACTCAAGTATCAAGGGCCAATTTTGAACTT
ACCAGTGTCACACAATTTGCTGCTCATCAAGAAAACAAGAGACTGGTTGGTTTTGTCATC
CGTGTTCCTGAATCCACTGGAGAAGAATCTCTGAGTACATACATTTTTGAAAGCAACTCA
GAAGGCGAAAAGATATGTTATGCTATTAATTTGGGAAAAGAAATTATTGAGGTTCAGAAG
GATCCAGAAGCACTGGCTCAATTAATGCTGTCCATACCACTAACCAATGATGGAAAATAT
GTACTGTTAAACGATCAACCAGATGACGATGATGGAAATCCAAATGAACATAGAGGCGCA
GAATCCGAAGCA

ORF Protein

Cloned ORF protein sequence for pFN21AB6205 Download

>KIBB6205  664 aa
MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQ
LSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFRE
KDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQ
YYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVEL
EAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERL
YFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESR
KENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNS
EMENENDKIVPKATASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDE
SFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVT
SQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNS
EGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGA
ESEA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6205 Download

>pFN21AB6205  6697 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGC
GTTGCAGGACAGCCCCCAGACTCGCTCTTTACTGAGCGTGTTTGAAGAAGATGCTGGCAC
CCTCACAGACTATACCAACCAGCTGCTCCAGGCAATGCAGCGCGTCTATGGAGCCCAGAA
TGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAACAGAA
CTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGT
GGTGGATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGT
TCTACCTATCATACAATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCT
ATTTGGACTCGCTAGCAATGAGCATGACCTCTCAATGGCAAAATACAGCAGGCTGCCTAA
GAAAAAGGAGAATGAGAAGGTGAAGACCGAAGTCGGAAAAGAGGTGGCCGCGGCCCGGCG
GAAGCAGCACCTCTCCTCCCTTCAGTACTACTGTGCCCTCAACGCGCTGCAGTACAGAAA
GCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCCCATGGACAGATTAACTTTTTTAA
GAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCCTCCGTTGCAGACAT
GGTTCAAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCA
AGAATTACTTTCTGTTGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACA
GATCAACAGGAACCTCATCCAGAAGGCTGGTTACCTTAATCTTAGAAACAAAACAGGGCT
GGTCACCACCACCTGGGAGAGGCTTTATTTCTTCACCCAAGGCGGGAATCTCATGTGTCA
GCCCAGGGGAGCCGTGGCTGGAGGTTTGATCCAGGACCTGGACAACTGCTCAGTGATGGC
CGTGGATTGCGAAGACCGGCGCTACTGCTTCCAGATCACCACGCCCAATGGAAAATCGGG
AATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAGTGGATATGTGCAATAAACAA
CATCTCCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATCAAGTTGAATCA
GACCGCTCTGCAAGCAGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATG
CCCCAGCCAGAACCTGAAAAATTCAGAGATGGAAAATGAAAATGACAAGATTGTTCCCAA
AGCAACAGCCAGTCTACCTGAAGCAGAGGAGCTGATCGCGCCTGGAACGCCGATTCAATT
CGATATTGTGCTTCCTGCTACAGAATTCCTTGATCAGAACAGAGGGAGCAGGCGTACCAA
CCCTTTTGGTGAAACTGAGGATGAATCATTTCCAGAAGCAGAAGATTCTCTTTTGCAGCA
GATGTTTATAGTTCGGTTTTTGGGATCAATGGCAGTTAAAACAGACAGCACTACTGAAGT
GATTTATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATTCATAACATCTTCCGCAT
GACAGAATCCCATCTGATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCACAGACTCA
AGTATCAAGGGCCAATTTTGAACTTACCAGTGTCACACAATTTGCTGCTCATCAAGAAAA
CAAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTGAATCCACTGGAGAAGAATCTCTGAG
TACATACATTTTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTATGCTATTAATTTGGG
AAAAGAAATTATTGAGGTTCAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTGTCCAT
ACCACTAACCAATGATGGAAAATATGTACTGTTAAACGATCAACCAGATGACGATGATGG
AAATCCAAATGAACATAGAGGCGCAGAATCCGAAGCAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT