Restriction map for
pFN21AB6205
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MroI MslI MunI NaeI
NarI NgoMIV NotI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1307 GTCTAC GT^[AC][GT]AC
AocI 1147 CCTGAGG CC^T[ACGT]AGG
Asp700I 1438 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1568 CTCGGG C^[CT]CG[GA]G
AvaII 969 GGACC G^G[AT]CC
BamHI 1860 GGATCC G^GATCC
BanI 91 GGCACC G^G[CT][GA]CC
BclI 1386 TGATCA T^GATCA
BcnI 509 CCCGG CC^[GC]GG
BglII 1127 AGATCT A^GATCT
BsaBI 1951 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 747 GTCCC GGGAC(10/14)
BsmI 704 GCATTC GAATGC(1/-1)
BsoBI 1568 CTCGGG C^[CT]CG[GA]G
BsrBI 1178 CCGCTC CCGCTC(-3/-3)
BsrFI 1010 ACCGGC [GA]^CCGG[CT]
Bsu36I 1147 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1010 ACCGGC [GA]^CCGG[CT]
DraI 384 TTTAAA TTT^AAA
DraII 968 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1090 GAAGAG CTCTTC(1/4)
Eco81I 1147 CCTGAGG CC^T[ACGT]AGG
EcoO109I 968 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1378 GAATTC G^AATTC
FbaI 1386 TGATCA T^GATCA
HaeII 1013 GGCGCT [GA]GCGC^[CT]
HgaI 137 GCGTC GACGC(5/10)
Ksp632I 1090 GAAGAG CTCTTC(1/4)
KspI 503 CCGCGG CCGC^GG
MaeII 660 ACGT A^CGT
MamI 1951 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 509 CCCGG CC^[GC]GG
NcoI 612 CCATGG C^CATGG
NdeI 200 CATATG CA^TATG
NheI 406 GCTAGC G^CTAGC
NruI 1157 TCGCGA TCG^CGA
PpuMI 968 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 480 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 562 CTGCAG CTGCA^G
PvuII 115 CAGCTG CAG^CTG
SacII 503 CCGCGG CCGC^GG
ScaI 539 AGTACT AGT^ACT
SmlI 1652 CTCAAG C^T[CT][GA]AG
TaqI 1355 TCGA T^CGA
TthHB8I 1355 TCGA T^CGA
XcmI 938 CCAGGGGAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1438 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1254 AAATTC [GA]^AATT[CT]
1378 GAATTC
ApoI 1254 AAATTC [GA]^AATT[CT]
1378 GAATTC
AseI 1825 ATTAAT AT^TAAT
1881 ATTAAT
AsnI 1825 ATTAAT AT^TAAT
1881 ATTAAT
BalI 167 TGGCCA TGG^CCA
581 TGGCCA
BanII 146 GAGCCC G[GA]GC[CT]^C
592 GAGCCC
BbsI 477 GAAGAC GAAGAC(2/6)
1006 GAAGAC
BfaI 25 CTAG C^TAG
407 CTAG
BpuAI 477 GAAGAC GAAGAC(2/6)
1006 GAAGAC
BsmAI 1551 GAGAC GTCTC(1/5)
1719 GAGAC
BsrDI 127 GCAATG GCAATG(2/0)
410 GCAATG
EcoNI 21 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
887 CCTGGGAGAGG
EcoT14I 612 CCATGG C^C[AT][AT]GG
912 CCAAGG
HapII 510 CCGG C^CGG
1011 CCGG
HpaII 510 CCGG C^CGG
1011 CCGG
MaeI 25 CTAG C^TAG
407 CTAG
MluNI 167 TGGCCA TGG^CCA
581 TGGCCA
MscI 167 TGGCCA TGG^CCA
581 TGGCCA
MspA1I 115 CAGCTG C[AC]G^C[GT]G
503 CCGCGG
MspI 510 CCGG C^CGG
1011 CCGG
PshBI 1825 ATTAAT AT^TAAT
1881 ATTAAT
SfaNI 85 GATGC GCATC(5/9)
738 GATGC
SfcI 562 CTGCAG C^T[GA][CT]AG
1373 CTACAG
StyI 612 CCATGG C^C[AT][AT]GG
912 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoO65I EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MvaI MvnI MwoI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII