(Circular) (Six-base) MAPSORT of: pFN21AB6205.seq Check: 9575 from: 1 to: 6697 pFN21AB6205 6697 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4507 278 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3313 4040 1937 Size: 1376 727 4594 Fragments arranged by size: 4594 1376 727 Acc65I G'GTAC_C Cuts at: 4018 4018 Size: 6697 AclI AA'CG_TT Cuts at: 1563 4824 5197 1563 Size: 3261 373 3063 Fragments arranged by size: 3261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 3339 3555 4760 6144 6412 1632 Size: 893 814 216 1205 1384 268 1917 Fragments arranged by size: 1917 1384 1205 893 814 268 216 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6500 AflIII A'CryG_T Cuts at: 1203 5596 6573 1203 Size: 4393 977 1327 Fragments arranged by size: 4393 1327 977 AgeI A'CCGG_T Cuts at: 5528 5528 Size: 6697 AhdI GACnn_n'nnGTC Cuts at: 5426 5426 Size: 6697 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6697 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6697 AlwNI CAG_nnn'CTG Cuts at: 2801 2977 3253 4440 6012 2801 Size: 176 276 1187 1572 3486 Fragments arranged by size: 3486 1572 1187 276 176 ApaLI G'TGCA_C Cuts at: 4754 5910 4754 Size: 1156 5541 ApoI r'AATT_y Cuts at: 1493 3259 3383 4006 4232 4384 6493 1493 Size: 1766 124 623 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 623 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 3831 3887 5251 160 Size: 872 2799 56 1364 1606 Fragments arranged by size: 2799 1606 1364 872 56 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 AvaI C'yCGr_G Cuts at: 1870 1943 3573 4022 1870 Size: 73 1630 449 4545 Fragments arranged by size: 4545 1630 449 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3645 3678 3645 Size: 33 6664 BamHI G'GATC_C Cuts at: 3865 4027 4394 3865 Size: 162 367 6168 Fragments arranged by size: 6168 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 4018 5473 618 Size: 325 217 627 309 1922 1455 1842 Fragments arranged by size: 1922 1842 1455 627 325 309 217 BanII G_rGCy'C Cuts at: 1391 2155 2601 4016 1391 Size: 764 446 1415 4072 Fragments arranged by size: 4072 1415 764 446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2489 3018 928 Size: 971 590 529 4607 Fragments arranged by size: 4607 971 590 529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4888 4922 1903 Size: 34 50 34 2867 34 3678 Fragments arranged by size: 3678 2867 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4589 5799 4589 Size: 1210 5487 BclI T'GATC_A Cuts at: 1548 3391 1548 Size: 1843 4854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5308 136 Size: 107 122 71 631 605 3636 1525 Fragments arranged by size: 3636 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3132 6692 3132 Size: 3560 3137 BlpI GC'TnA_GC Cuts at: 4114 4114 Size: 6697 Bme1580I G_kGCm'C Cuts at: 1790 2557 4758 5914 1790 Size: 767 2201 1156 2573 Fragments arranged by size: 2573 2201 1156 767 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6697 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4428 5386 442 Size: 416 53 83 399 24 3011 958 1753 Fragments arranged by size: 3011 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2415 1056 Size: 1359 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2778 2979 3053 3109 3783 5357 1239 Size: 872 667 201 74 56 674 1574 2579 Fragments arranged by size: 2579 1574 872 674 667 201 74 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3642 4822 5687 5985 3642 Size: 1180 865 298 4354 Fragments arranged by size: 4354 1180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5360 882 Size: 617 3861 2219 Fragments arranged by size: 3861 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4488 493 Size: 711 3284 2702 Fragments arranged by size: 3284 2702 711 BsaBI GATnn'nnATC Cuts at: 3960 6691 3960 Size: 2731 3966 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4504 4886 5521 275 Size: 53 83 186 936 2971 382 635 1451 Fragments arranged by size: 2971 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5130 5528 5802 5949 6360 5130 Size: 398 274 147 411 5467 Fragments arranged by size: 5467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 3107 3137 3397 Size: 30 165 30 1116 30 558 30 260 Cuts at: 3397 3427 1178 Size: 30 4448 Fragments arranged by size: 4448 1116 558 260 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2524 3341 1186 Size: 862 476 817 4542 Fragments arranged by size: 4542 862 817 476 BseYI C'CCAG_C Cuts at: 2754 3242 5900 6396 2754 Size: 488 2658 496 3055 Fragments arranged by size: 3055 2658 496 488 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6124 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4147 4908 5936 1540 Size: 275 186 2146 761 1028 2301 Fragments arranged by size: 2301 2146 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4758 4843 5914 4758 Size: 85 1071 5541 Fragments arranged by size: 5541 1071 85 BsmI GAATG_Cn' Cuts at: 2709 4208 4301 2709 Size: 1499 93 5105 Fragments arranged by size: 5105 1499 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2557 2601 4016 4758 4843 5914 Size: 399 365 402 44 1415 742 85 1071 Cuts at: 5914 1391 Size: 2174 Fragments arranged by size: 2174 1415 1071 742 402 399 365 85 44 BspEI T'CCGG_A Cuts at: 6360 6360 Size: 6697 BspHI T'CATG_A Cuts at: 4586 6316 4586 Size: 1730 4967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4052 844 Size: 3208 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3185 4584 1394 Size: 429 1362 1399 3507 Fragments arranged by size: 3507 1399 1362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2139 2422 5192 5366 65 Size: 1183 891 283 2770 174 1396 Fragments arranged by size: 2770 1396 1183 891 283 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3015 5341 5528 1700 Size: 110 1205 2326 187 2869 Fragments arranged by size: 2869 2326 1205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6697 BssSI C'ACGA_G Cuts at: 4757 5769 4757 Size: 1012 5685 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6697 BstBI TT'CG_AA Cuts at: 5501 5501 Size: 6697 BstEII G'GTnAC_C Cuts at: 1184 2849 2881 3619 1184 Size: 1665 32 738 4262 Fragments arranged by size: 4262 1665 738 32 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2668 2846 2281 Size: 387 178 6132 Fragments arranged by size: 6132 387 178 BstYI r'GATC_y Cuts at: 1972 2368 2395 3132 3646 3865 4027 4394 4779 Size: 396 27 737 514 219 162 367 385 Cuts at: 4779 4796 6248 6334 6346 6692 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 737 514 396 385 367 346 219 162 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 3153 3153 Size: 6697 BtgI C'CryG_G Cuts at: 513 1065 2013 2508 2617 2952 3000 513 Size: 552 948 495 109 335 48 4210 Fragments arranged by size: 4210 948 552 495 335 109 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3566 487 Size: 683 56 2340 3618 Fragments arranged by size: 3618 2340 683 56 BtsI GCAGTG_nn' Cuts at: 3201 4213 5007 5027 3201 Size: 1012 794 20 4871 Fragments arranged by size: 4871 1012 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6662 DraI TTT'AAA Cuts at: 2391 4001 2391 Size: 1610 5087 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5704 809 Size: 507 4388 1802 Fragments arranged by size: 4388 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2505 2586 Size: 54 1425 50 275 18 342 333 81 Cuts at: 2586 2808 2997 4144 5033 6662 8 Size: 222 189 1147 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 342 333 275 222 189 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4144 1537 Size: 275 2332 4090 Fragments arranged by size: 4090 2332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3090 4627 1353 Size: 1737 1537 3423 Fragments arranged by size: 3423 1737 1537 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2533 2750 5284 5658 5804 387 Size: 1064 138 944 217 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 944 374 217 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2525 2778 2979 3053 3109 3339 Size: 393 479 414 253 201 74 56 230 Cuts at: 3339 3555 3783 4760 5357 6144 6412 1239 Size: 216 228 977 597 787 268 1524 Fragments arranged by size: 1524 977 787 597 479 414 393 268 253 230 228 216 201 74 56 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2896 2030 Size: 866 5831 EcoO109I rG'GnC_Cy Cuts at: 2974 2974 Size: 6697 EcoRI G'AATT_C Cuts at: 3383 4006 4384 3383 Size: 623 378 5696 Fragments arranged by size: 5696 623 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2783 3485 2783 Size: 702 5995 FspI TGC'GCA Cuts at: 5203 5203 Size: 6697 HaeII r_GCGC'y Cuts at: 1678 3022 5844 1678 Size: 1344 2822 2531 Fragments arranged by size: 2822 2531 1344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2952 2972 2984 3004 3428 Size: 32 135 32 1380 20 12 20 424 Cuts at: 3428 3460 5338 5370 5412 5444 1373 Size: 32 1878 32 42 32 2626 Fragments arranged by size: 2626 1878 1380 424 135 42 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 4041 4287 669 Size: 988 281 2103 246 3079 Fragments arranged by size: 3079 2103 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6697 HpaI GTT'AAC Cuts at: 4287 4287 Size: 6697 KpnI G_GTAC'C Cuts at: 4022 4022 Size: 6697 MfeI C'AATT_G Cuts at: 4296 4296 Size: 6697 MluI A'CGCG_T Cuts at: 6573 6573 Size: 6697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2473 5811 5995 1305 Size: 1168 3338 184 2007 Fragments arranged by size: 3338 2007 1168 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2588 10 Size: 54 1425 343 342 414 4119 Fragments arranged by size: 4119 1425 414 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 4655 5014 5173 518 Size: 648 3489 359 159 2042 Fragments arranged by size: 3489 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2510 4113 4790 5938 6183 1606 Size: 516 388 1603 677 1148 245 2120 Fragments arranged by size: 2120 1603 1148 677 516 388 245 NcoI C'CATG_G Cuts at: 513 1065 2617 513 Size: 552 1552 4593 Fragments arranged by size: 4593 1552 552 NdeI CA'TA_TG Cuts at: 387 2206 387 Size: 1819 4878 NheI G'CTAG_C Cuts at: 1052 2411 1052 Size: 1359 5338 NotI GC'GGCC_GC Cuts at: 4144 4144 Size: 6697 NruI TCG'CGA Cuts at: 3164 3164 Size: 6697 NspI r_CATG'y Cuts at: 1132 1229 4055 5600 1132 Size: 97 2826 1545 2229 Fragments arranged by size: 2826 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2943 1348 Size: 1595 5102 PciI A'CATG_T Cuts at: 5596 5596 Size: 6697 PflMI CCAn_nnn'nTGG Cuts at: 1108 2892 2977 3616 1108 Size: 1784 85 639 4189 Fragments arranged by size: 4189 1784 639 85 PfoI T'CCnGG_A Cuts at: 2970 2970 Size: 6697 PmeI GTTT'AAAC Cuts at: 4001 4001 Size: 6697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4766 4798 6279 6311 4766 Size: 32 1481 32 5152 Fragments arranged by size: 5152 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2974 2974 Size: 6697 PshAI GACnn'nnGTC Cuts at: 1613 2489 1613 Size: 876 5821 PsiI TTA'TAA Cuts at: 4267 4267 Size: 6697 PstI C_TGCA'G Cuts at: 830 2571 4049 830 Size: 1741 1478 3478 Fragments arranged by size: 3478 1741 1478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6697 PvuII CAG'CTG Cuts at: 2122 2122 Size: 6697 SacII CC_GC'GG Cuts at: 2511 2511 Size: 6697 SalI G'TCGA_C Cuts at: 1936 4039 1936 Size: 2103 4594 SbfI CC_TGCA'GG Cuts at: 4049 4049 Size: 6697 ScaI AGT'ACT Cuts at: 2546 4945 6657 2546 Size: 2399 1712 2586 Fragments arranged by size: 2586 2399 1712 SfcI C'TryA_G Cuts at: 826 1045 2567 3378 4045 5180 5861 6052 826 Size: 219 1522 811 667 1135 681 191 1471 Fragments arranged by size: 1522 1471 1135 811 681 667 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6697 SmaI CCC'GGG Cuts at: 1872 4024 1872 Size: 2152 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3657 4801 5702 5964 820 Size: 197 926 1714 1144 901 262 1553 Fragments arranged by size: 1714 1553 1144 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4488 493 Size: 3995 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6697 SphI G_CATG'C Cuts at: 1132 4055 1132 Size: 2923 3774 SspI AAT'ATT Cuts at: 5 52 4621 5 Size: 47 4569 2081 Fragments arranged by size: 4569 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6697 StyI C'CwwG_G Cuts at: 513 1065 2617 2917 4136 513 Size: 552 1552 300 1219 3074 Fragments arranged by size: 3074 1552 1219 552 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 2930 3158 4724 4741 4894 5079 Size: 1255 921 429 228 1566 17 153 185 Cuts at: 5079 5524 325 Size: 445 1498 Fragments arranged by size: 1566 1498 1255 921 445 429 228 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 2571 3779 Size: 274 80 33 51 1443 567 27 1208 Cuts at: 3779 3925 4943 6655 96 Size: 146 1018 1712 138 Fragments arranged by size: 1712 1443 1208 1018 567 274 146 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2255 2287 2487 2519 2551 6657 6689 2255 Size: 32 200 32 32 4106 32 2263 Fragments arranged by size: 4106 2263 200 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6697 XbaI T'CTAG_A Cuts at: 4033 4033 Size: 6697 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2950 2011 Size: 939 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6697 XmaI C'CCGG_G Cuts at: 1870 4022 1870 Size: 2152 4545 XmnI GAAnn'nnTTC Cuts at: 3447 4826 3447 Size: 1379 5318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4505 276 Size: 53 83 186 936 2971 2468 Fragments arranged by size: 2971 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI