LMX1A ORF clone FHC03263

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03263

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Clone info.

Species

human

Clone Name

pFN21AB9714 Sequence (with vector) Restriction Map

Product ID

FHC03263

Flexi Clone PID

FXC03263

ORF Sequence

DNA sequence (1146 bp)
Protein sequence (382 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463837]

Gene

KIBB9714

Symbol

LMX1A

Alias : DFNA7, LMX1, LMX1.1

Description

LIM homeobox transcription factor 1, alpha, transcript variant 1

Original Clone ID

cp00297

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1146 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 382 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1247.1 1.6e-97 100.0 LMX1A CCDS55342.1 3.5e-65 68.7 LMX1B CCDS6866.2 2.5e-62 67.2 LMX1B CCDS55343.1 5e-57 66.2 LMX1B CCDS10290.1 8.1e-19 38.7 ISL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001781 35 90 PF00412 Zinc finger IPR001781 94 151 PF00412 Zinc finger IPR001356 196 252 PF00046 Homeobox HMMSmart IPR001781 34 85 SM00132 Zinc finger IPR001781 93 147 SM00132 Zinc finger IPR001356 195 257 SM00389 Homeobox ProfileScan IPR001781 33 92 PS50023 Zinc finger IPR001781 93 154 PS50023 Zinc finger IPR001356 193 253 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001167540 382 600298 100.0 100.0 NP_796372 382 600298 100.0 100.0 NP_001167618 402 161200,602575 68.7 100.0 NP_002307 395 161200,602575 67.2 100.0 NP_001167617 406 161200,602575 66.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9714 Download

>KIBB9714  1146 bp
ATGCTGGACGGCCTAAAGATGGAGGAGAACTTCCAAAGCGCGATCGACACCTCGGCCTCC
TTCTCCTCGCTGCTGGGCAGAGCGGTGAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGG
GTCATCTTGGACAGGTTTCTGCTGCGGCTCAACGACAGCTTCTGGCATGAGCAGTGCGTG
CAGTGCGCCTCCTGCAAAGAGCCCCTGGAGACCACCTGCTTCTACCGGGACAAGAAGCTG
TACTGCAAGTATGACTACGAGAAGCTGTTTGCTGTTAAATGTGGGGGCTGCTTCGAGGCC
ATCGCTCCCAATGAGTTTGTTATGCGGGCCCAGAAGAGTGTATACCACCTGAGCTGCTTC
TGCTGCTGTGTCTGCGAGCGACAGCTTCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGG
CAGCTGCTCTGCAAAGGGGACTATGAGAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCA
GCAGCCTCAGACTCAGGTAAAAGTGATGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGG
GCAGGGAAAGGAACTGCTGAGGAAGGCAAGGACCATAAGCGCCCCAAACGTCCGAGAACC
ATCTTGACAACTCAACAGAGGCGAGCATTCAAGGCCTCATTTGAAGTATCCTCCAAGCCC
TGCAGGAAGGTGAGAGAGACTCTGGCTGCAGAGACAGGGCTGAGTGTCCGTGTCGTCCAG
GTGTGGTTCCAAAACCAGAGAGCGAAGATGAAGAAGCTGGCCAGGCGACAGCAGCAGCAG
CAGCAAGATCAGCAGAACACCCAGAGGCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGT
GCTGGGATGGAAGGAATCATGAACCCCTACACGGCTCTGCCCACCCCACAGCAGCTCCTG
GCCATCGAGCAGAGTGTCTACAGCTCAGATCCCTTCCGACAGGGTCTCACCCCACCCCAG
ATGCCTGGAGACCACATGCACCCTTATGGTGCCGAGCCCCTTTTCCATGACCTGGATAGC
GACGACACCTCCCTCAGTAACCTGGGTGACTGTTTCCTAGCAACCTCAGAAGCTGGGCCT
CTGCAGTCCAGAGTGGGAAACCCCATTGACCATCTGTACTCCATGCAGAATTCTTACTTC
ACATCT

ORF Protein

Cloned ORF protein sequence for pFN21AB9714 Download

>KIBB9714  382 aa
MLDGLKMEENFQSAIDTSASFSSLLGRAVSPKSVCEGCQRVILDRFLLRLNDSFWHEQCV
QCASCKEPLETTCFYRDKKLYCKYDYEKLFAVKCGGCFEAIAPNEFVMRAQKSVYHLSCF
CCCVCERQLQKGDEFVLKEGQLLCKGDYEKERELLSLVSPAASDSGKSDDEESLCKSAHG
AGKGTAEEGKDHKRPKRPRTILTTQQRRAFKASFEVSSKPCRKVRETLAAETGLSVRVVQ
VWFQNQRAKMKKLARRQQQQQQDQQNTQRLSSAQTNGGGSAGMEGIMNPYTALPTPQQLL
AIEQSVYSSDPFRQGLTPPQMPGDHMHPYGAEPLFHDLDSDDTSLSNLGDCFLATSEAGP
LQSRVGNPIDHLYSMQNSYFTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9714 Download

>pFN21AB9714  5851 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGGACGGCCTAAAGATGGAGGAGAACTTCCA
AAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGCTGCTGGGCAGAGCGGTGAGCCCCAA
GTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCTGCTGCGGCTCAACGA
CAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCTGGAGACCAC
CTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCTGTTTGCTGT
TAAATGTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCGGGCCCAGAA
GAGTGTATACCACCTGAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCTTCAGAAGGG
TGATGAGTTTGTCCTGAAGGAGGGGCAGCTGCTCTGCAAAGGGGACTATGAGAAGGAGCG
GGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAGACTCAGGTAAAAGTGATGATGAAGA
AAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGAGGAAGGCAAGGACCA
TAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGCATTCAAGGC
CTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGCTGCAGAGAC
AGGGCTGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAAGATGAAGAA
GCTGGCCAGGCGACAGCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAGGCTGAGCTC
TGCTCAGACAAACGGTGGTGGGAGTGCTGGGATGGAAGGAATCATGAACCCCTACACGGC
TCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGCAGAGTGTCTACAGCTCAGATCCCTT
CCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCACCCTTATGGTGCCGA
GCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGGTGACTGTTT
CCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCATTGACCATCT
GTACTCCATGCAGAATTCTTACTTCACATCTGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT