Restriction map for
pFN21AB9714
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BsrI BssHII BssSI
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 39 CGCG CG^CG
AcsI 1128 GAATTC [GA]^AATT[CT]
AlwI 928 GATCC GGATC(4/5)
ApaI 326 GGGCCC GGGCC^C
ApoI 1128 GAATTC [GA]^AATT[CT]
AspHI 810 GAGCTC G[AT]GC[AT]^C
AspI 938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 570 GGACC G^G[AT]CC
BanI 988 GGTGCC G^G[CT][GA]CC
BbvCI 556 GCTGAGG CCTCAGC(-5/-2)
BciVI 646 GTATCC GTATCC(6/5)
BcnI 225 CCGGG CC^[GC]GG
BfaI 1057 CTAG C^TAG
BglI 532 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 178 GTGCAG GTGCAG(16/14)
BsiEI 41 CGATCG CG[GA][CT]^CG
BsiHKAI 810 GAGCTC G[AT]GC[AT]^C
BsmI 625 GCATTC GAATGC(1/-1)
BspHI 857 TCATGA T^CATGA
BspMI 214 ACCTGC ACCTGC(4/8)
Bst1107I 340 GTATAC GTA^TAC
BstAPI 171 GCAGTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 845 GGATG GGATG(2/0)
BstUI 39 CGCG CG^CG
BstXI 957 CCAGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 927 AGATCC [GA]^GATC[CT]
BstZ17I 340 GTATAC GTA^TAC
DsaI 534 CCATGG C^C[GA][CT]GG
EarI 333 GAAGAG CTCTTC(1/4)
EcoNI 408 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1128 GAATTC G^AATTC
EcoT14I 534 CCATGG C^C[AT][AT]GG
FokI 845 GGATG GGATG(9/13)
HaeII 578 AGCGCC [GA]GCGC^[CT]
HapII 225 CCGG C^CGG
HpaII 225 CCGG C^CGG
Ksp632I 333 GAAGAG CTCTTC(1/4)
MaeI 1057 CTAG C^TAG
MaeII 588 ACGT A^CGT
MflI 927 AGATCC [GA]^GATC[CT]
MseI 275 TTAA T^TAA
MslI 979 CACCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 225 CCGG C^CGG
MvnI 39 CGCG CG^CG
NciI 225 CCGGG CC^[GC]GG
NcoI 534 CCATGG C^CATGG
NspI 974 ACATGC [GA]CATG^[CT]
PflFI 938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1005 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 41 CGATCG CGAT^CG
PvuII 421 CAGCTG CAG^CTG
RcaI 857 TCATGA T^CATGA
SacI 810 GAGCTC GAGCT^C
SbfI 659 CCTGCAGG CCTGCA^GG
SfaNI 960 GATGC GCATC(5/9)
Sse8387I 659 CCTGCAGG CCTGCA^GG
StuI 632 AGGCCT AGG^CCT
StyI 534 CCATGG C^C[AT][AT]GG
TfiI 854 GAATC G^A[AT]TC
Tru9I 275 TTAA T^TAA
Tsp45I 1046 GTGAC ^GT[GC]AC
Tsp509I 1129 AATT ^AATT
Tth111I 938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1005 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 927 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 340 GTATAC GT^[AC][GT]AC
916 GTCTAC
AfaI 240 GTAC GT^AC
1116 GTAC
BalI 758 TGGCCA TGG^CCA
899 TGGCCA
BlpI 462 GCTCAGC GC^T[ACGT]AGC
807 GCTGAGC
BpmI 205 CTGGAG CTGGAG(16/14)
965 CTGGAG
Bpu1102I 462 GCTCAGC GC^T[ACGT]AGC
807 GCTGAGC
BseRI 63 CTCCTC GAGGAG(10/8)
22 GAGGAG
BsmFI 227 GGGAC GGGAC(10/14)
437 GGGAC
BsrBI 80 GAGCGG CCGCTC(-3/-3)
451 GAGCGG
CelII 462 GCTCAGC GC^T[ACGT]AGC
807 GCTGAGC
EaeI 758 TGGCCA [CT]^GGCC[GA]
899 TGGCCA
MaeIII 1037 GTAAC ^GT[ACGT]AC
1046 GTGAC
MluNI 758 TGGCCA TGG^CCA
899 TGGCCA
MscI 758 TGGCCA TGG^CCA
899 TGGCCA
MspA1I 115 CAGCGG C[AC]G^C[GT]G
421 CAGCTG
PleI 490 GACTC GAGTC(4/5)
678 GACTC
RsaI 240 GTAC GT^AC
1116 GTAC
TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^
181 CAGTG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BmyI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PstI Sau3AI Sau96I ScrFI
SfcI TaqI TseI TthHB8I