(Circular) (Six-base) MAPSORT of: pFN21AB9714.seq Check: 219 from: 1 to: 5851 pFN21AB9714 5851 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2228 2228 Size: 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 2922 3194 1937 Size: 409 576 272 4594 Fragments arranged by size: 4594 576 409 272 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2435 3914 5298 5566 1632 Size: 743 60 1479 1384 268 1917 Fragments arranged by size: 1917 1479 1384 743 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2166 2812 2902 3077 3594 5166 2166 Size: 646 90 175 517 1572 2851 Fragments arranged by size: 2851 1572 646 517 175 90 ApaI G_GGCC'C Cuts at: 2335 2335 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3133 3160 3386 3538 5647 1493 Size: 1640 27 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 3176 1870 Size: 73 1233 4545 Fragments arranged by size: 4545 1233 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2993 3172 4627 618 Size: 325 217 627 1206 179 1455 1842 Fragments arranged by size: 1842 1455 1206 627 325 217 179 BanII G_rGCy'C Cuts at: 1391 2096 2208 2335 2483 2819 3003 3170 1391 Size: 705 112 127 148 336 184 167 4072 Fragments arranged by size: 4072 705 336 184 167 148 127 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BbvCI CC'TCA_GC Cuts at: 2562 2562 Size: 5851 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4042 4076 1903 Size: 34 50 34 2021 34 3678 Fragments arranged by size: 3678 2021 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2662 3743 4953 2662 Size: 1081 1210 3560 Fragments arranged by size: 3560 1210 1081 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2543 4462 136 Size: 107 122 71 631 605 871 1919 1525 Fragments arranged by size: 1919 1525 871 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 2468 2813 3268 2468 Size: 345 455 5051 Fragments arranged by size: 5051 455 345 Bme1580I G_kGCm'C Cuts at: 1790 2335 3912 5068 1790 Size: 545 1577 1156 2573 Fragments arranged by size: 2573 1577 1156 545 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2700 2838 2700 Size: 138 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2991 4511 1239 Size: 992 760 1520 2579 Fragments arranged by size: 2579 1520 992 760 Bpu10I CC'TnA_GC Cuts at: 2354 2562 2354 Size: 208 5643 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 2954 2967 4514 882 Size: 617 708 747 13 1547 2219 Fragments arranged by size: 2219 1547 747 708 617 13 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2883 2913 3017 3047 1178 Size: 30 165 30 1480 30 104 30 3982 Fragments arranged by size: 3982 1480 165 104 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2059 1186 Size: 856 17 4978 Fragments arranged by size: 4978 856 17 BseYI C'CCAG_C Cuts at: 2077 2482 2846 3077 5054 5550 2077 Size: 405 364 231 1977 496 2378 Fragments arranged by size: 2378 1977 496 405 364 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 1163 Size: 573 468 4810 Fragments arranged by size: 4810 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3301 4062 5090 1540 Size: 275 186 48 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2819 3912 3997 5068 2819 Size: 1093 85 1071 3602 Fragments arranged by size: 3602 1093 1071 85 BsmI GAATG_Cn' Cuts at: 2630 3362 3455 2630 Size: 732 93 5026 Fragments arranged by size: 5026 732 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2208 2335 2483 2819 3003 3170 Size: 399 306 112 127 148 336 184 167 Cuts at: 3170 3912 3997 5068 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 306 184 167 148 127 112 85 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 2862 3740 5470 2862 Size: 878 1730 3243 Fragments arranged by size: 3243 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2228 3206 844 Size: 1384 978 3489 Fragments arranged by size: 3489 1384 978 BsrBI CCG'CTC Cuts at: 1394 1823 2087 2458 3738 1394 Size: 429 264 371 1280 3507 Fragments arranged by size: 3507 1280 429 371 264 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 2182 794 Size: 1388 4463 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 2969 2969 Size: 5851 BstYI r'GATC_y Cuts at: 1972 2932 3181 3548 3933 3950 5402 5488 5500 Size: 960 249 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 960 385 367 346 249 86 17 12 BstZ17I GTA'TAC Cuts at: 2347 2347 Size: 5851 BtgI C'CryG_G Cuts at: 513 1065 2539 513 Size: 552 1474 3825 Fragments arranged by size: 3825 1474 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2290 487 Size: 683 56 1064 4048 Fragments arranged by size: 4048 1064 683 56 BtsI GCAGTG_nn' Cuts at: 2183 2192 3367 4161 4181 2183 Size: 9 1175 794 20 3853 Fragments arranged by size: 3853 1175 794 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 2904 3298 Size: 54 1425 50 275 18 933 141 394 Cuts at: 3298 4187 5816 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 933 889 394 275 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 3781 1353 Size: 980 1448 3423 Fragments arranged by size: 3423 1448 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2817 2817 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2375 2435 2991 3914 4511 5298 Size: 393 599 144 60 556 923 597 787 Cuts at: 5298 5566 1239 Size: 268 1524 Fragments arranged by size: 1524 923 787 599 597 556 393 268 144 60 EcoNI CCTnn'n_nnAGG Cuts at: 2417 2417 Size: 5851 EcoRI G'AATT_C Cuts at: 3133 3160 3538 3133 Size: 27 378 5446 Fragments arranged by size: 5446 378 27 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2587 4998 1678 Size: 909 2411 2531 Fragments arranged by size: 2531 2411 909 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2934 2966 3017 3049 4492 Size: 32 135 32 1362 32 51 32 1443 Cuts at: 4492 4524 4566 4598 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 1362 135 51 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2965 4965 5149 1305 Size: 1660 2000 184 2007 Fragments arranged by size: 2007 2000 1660 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 2906 10 Size: 54 1425 343 933 141 2955 Fragments arranged by size: 2955 1425 933 343 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2988 3809 4168 4327 518 Size: 648 1822 821 359 159 2042 Fragments arranged by size: 2042 1822 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2428 3267 3944 5092 5337 1606 Size: 516 306 839 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 677 516 306 245 NcoI C'CATG_G Cuts at: 513 1065 2539 513 Size: 552 1474 3825 Fragments arranged by size: 3825 1474 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2983 3209 4754 1132 Size: 97 1754 226 1545 2229 Fragments arranged by size: 2229 1754 1545 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 3016 1108 Size: 1908 3943 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2331 2331 Size: 5851 PstI C_TGCA'G Cuts at: 830 2669 2695 3090 3203 830 Size: 1839 26 395 113 3478 Fragments arranged by size: 3478 1839 395 113 26 PvuI CG_AT'CG Cuts at: 2001 2049 2001 Size: 48 5803 PvuII CAG'CTG Cuts at: 2428 2428 Size: 5851 SacI G_AGCT'C Cuts at: 2819 2819 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 2669 3203 2669 Size: 534 5317 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2665 2691 2923 3086 3199 4334 5015 Size: 219 1620 26 232 163 113 1135 681 Cuts at: 5015 5206 826 Size: 191 1471 Fragments arranged by size: 1620 1471 1135 681 232 219 191 163 113 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3955 4856 5118 820 Size: 197 926 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 2639 1517 Size: 1122 4729 StyI C'CwwG_G Cuts at: 513 1065 2539 3290 513 Size: 552 1474 751 3074 Fragments arranged by size: 3074 1474 751 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2819 3038 3878 3895 4048 4233 4678 Size: 1255 1239 219 840 17 153 185 445 Cuts at: 4678 325 Size: 1498 Fragments arranged by size: 1498 1255 1239 840 445 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 3120 4097 Size: 274 80 33 51 1443 267 876 977 Cuts at: 4097 5809 96 Size: 1712 138 Fragments arranged by size: 1712 1443 977 876 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2883 2915 5811 5843 2883 Size: 32 2896 32 2891 Fragments arranged by size: 2896 2891 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2946 1316 Size: 1630 4221 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI