GAB3 ORF clone FHC02361

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02361

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AB7259 Sequence (with vector) Restriction Map

Product ID

FHC02361

Flexi Clone PID

FXC02361

ORF Sequence

DNA sequence (1758 bp)
Protein sequence (586 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463697]

Gene

KIBB7259

Symbol

GAB3

Description

GRB2-associated binding protein 3, transcript variant 2

Original Clone ID

oc00475

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1758 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 586 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14760.1 4.9e-172 100.0 GAB3 CCDS48198.1 1.7e-171 99.8 GAB3 CCDS65357.1 1.3e-149 93.2 GAB3 CCDS8259.1 4e-20 33.7 GAB2 CCDS3759.1 6.8e-20 31.3 GAB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 8 116 PF00169 Pleckstrin homology domain HMMSmart IPR001849 6 119 SM00233 Pleckstrin homology domain ProfileScan IPR001849 5 117 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_542179 586 300482 100.0 100.0 NP_001075042 587 300482 99.8 100.0 XP_005274705 587 300482 99.8 100.0 XP_006724867 587 300482 99.8 100.0 XP_016884765 609 300482 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7259 Download

>KIBB7259  1758 bp
ATGAGTGCGGGCGACGCAGTGTGCACCGGCTGGCTCGTTAAGTCGCCCCCCGAGAGGAAG
CTACAGCGCTACGCCTGGCGCAAGCGCTGGTTTGTCCTCCGGCGAGGCCGCATGAGCGGC
AACCCCGATGTCTTGGAGTACTACAGGAACAAGCACTCCAGCAAGCCCATCCGGGTGATA
GACCTCAGCGAGTGTGCAGTGTGGAAGCATGTGGGCCCCAGCTTTGTTCGGAAGGAATTT
CAGAATAATTTCGTGTTCATTGTCAAGACTACTTCCCGTACATTCTACCTGGTGGCCAAA
ACTGAGCAAGAAATGCAGGTGTGGGTGCACAGCATCAGTCAGGTCTGCAACCTTGGCCAC
CTGGAGGATGGTGCAGATTCCATGGAGAGCCTCTCTTACACGCCCTCCTCCCTGCAGCCA
TCCTCTGCCAGCTCCCTTCTTACCGCCCATGCTGCCAGCTCCTCTTTGCCAAGAGATGAC
CCAAACACTAATGCCGTAGCCACTGAGGAAACCAGAAGTGAGTCAGAGCTTCTCTTCCTT
CCAGATTATCTGGTTTTGTCCAACTGCGAGACTGGAAGACTGCACCATACCAGTCTACCC
ACCAGATGTGATAGCTGGTCAAACTCAGACCGTTCATTGGAACAGGCTTCATTTGATGAT
GTTTTTGTTGACTGCCTGCAGCCGCTCCCCTCCAGTCATTTGGTCCACCCCTCATGCCAT
GGCAGTGGAGCTCAGGAGGTGCCATCCTCGAGGCCTCAGGCTGCCCTGATCTGGAGTAGA
GAAATCAATGGGCCACCCAGGGACCACTTGTCTTCTTCACCATTGCTGGAAAGTTCCTTA
AGTTCCACCATTCAGGTAGATAAAAATCAAGGTTCCTTACCCTGTGGAGCAAAAGAACTA
GACATTATGTCCAACACTCCACCTCCCCGCCCCCCTAAGCCAAGCCATCTGTCTGAACGG
CGCCAAGAGGAGTGGAGTACACACAGTGGTAGCAAGAAGCCAGAATGCACTCTGGTTCCA
AGAAGAATCTCCCTCTCTGGTTTAGACAACATGAGAACCTGGAAAGCTGATGTAGAAGGC
CAATCCTTAAGACACCGAGACAAGCGGCTTAGTTTGAATTTGCCATGCAGGTTCTCCCCG
ATGTACCCCACAGCTTCAGCCAGTATCGAAGACAGCTATGTGCCCATGAGCCCCCAGGCT
GGTGCCTCTGGTCTTGGACCCCACTGCAGCCCTGATGACTACATTCCAATGAACTCAGGA
AGCATCTCAAGCCCGTTGCCTGAGCTGCCTGCAAACCTGGAACCTCCCCCAGTGAATAGA
GATCTCAAGCCTCAGAGGAAATCACGGCCACCTCCTCTGGACCTGAGAAACCTCTCGATC
ATCCGGGAACATGCATCTCTTACCAGGACCCGCACTGTGCCTTGCAGTCGAACCAGCTTT
CTCTCTCCAGAAAGAAATGGTATTAATTCTGCAAGATTTTTTGCTAATCCTGTTTCCAGA
GAAGACGAAGAAAGCTACATCGAAATGGAGGAGCACCGAACAGCCAGTTCCCTGAGCAGT
GGTGCCCTTACGTGGACAAAGAAATTCAGCCTAGATTATTTGGCCCTGGACTTCAATTCA
GCATCACCAGCCCCCATGCAGCAGAAACTTCTCCTTTCAGAAGAACAAAGAGTAGACTAT
GTCCAAGTGGATGAGCAGAAGACACAGGCTCTCCAGAGCACAAAACAGGAGTGGACGGAT
GAAAGGCAATCCAAAGTA

ORF Protein

Cloned ORF protein sequence for pFN21AB7259 Download

>KIBB7259  586 aa
MSAGDAVCTGWLVKSPPERKLQRYAWRKRWFVLRRGRMSGNPDVLEYYRNKHSSKPIRVI
DLSECAVWKHVGPSFVRKEFQNNFVFIVKTTSRTFYLVAKTEQEMQVWVHSISQVCNLGH
LEDGADSMESLSYTPSSLQPSSASSLLTAHAASSSLPRDDPNTNAVATEETRSESELLFL
PDYLVLSNCETGRLHHTSLPTRCDSWSNSDRSLEQASFDDVFVDCLQPLPSSHLVHPSCH
GSGAQEVPSSRPQAALIWSREINGPPRDHLSSSPLLESSLSSTIQVDKNQGSLPCGAKEL
DIMSNTPPPRPPKPSHLSERRQEEWSTHSGSKKPECTLVPRRISLSGLDNMRTWKADVEG
QSLRHRDKRLSLNLPCRFSPMYPTASASIEDSYVPMSPQAGASGLGPHCSPDDYIPMNSG
SISSPLPELPANLEPPPVNRDLKPQRKSRPPPLDLRNLSIIREHASLTRTRTVPCSRTSF
LSPERNGINSARFFANPVSREDEESYIEMEEHRTASSLSSGALTWTKKFSLDYLALDFNS
ASPAPMQQKLLLSEEQRVDYVQVDEQKTQALQSTKQEWTDERQSKV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7259 Download

>pFN21AB7259  6463 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGTGCGGGCGACGCAGTGTGCACCGGCTGGCT
CGTTAAGTCGCCCCCCGAGAGGAAGCTACAGCGCTACGCCTGGCGCAAGCGCTGGTTTGT
CCTCCGGCGAGGCCGCATGAGCGGCAACCCCGATGTCTTGGAGTACTACAGGAACAAGCA
CTCCAGCAAGCCCATCCGGGTGATAGACCTCAGCGAGTGTGCAGTGTGGAAGCATGTGGG
CCCCAGCTTTGTTCGGAAGGAATTTCAGAATAATTTCGTGTTCATTGTCAAGACTACTTC
CCGTACATTCTACCTGGTGGCCAAAACTGAGCAAGAAATGCAGGTGTGGGTGCACAGCAT
CAGTCAGGTCTGCAACCTTGGCCACCTGGAGGATGGTGCAGATTCCATGGAGAGCCTCTC
TTACACGCCCTCCTCCCTGCAGCCATCCTCTGCCAGCTCCCTTCTTACCGCCCATGCTGC
CAGCTCCTCTTTGCCAAGAGATGACCCAAACACTAATGCCGTAGCCACTGAGGAAACCAG
AAGTGAGTCAGAGCTTCTCTTCCTTCCAGATTATCTGGTTTTGTCCAACTGCGAGACTGG
AAGACTGCACCATACCAGTCTACCCACCAGATGTGATAGCTGGTCAAACTCAGACCGTTC
ATTGGAACAGGCTTCATTTGATGATGTTTTTGTTGACTGCCTGCAGCCGCTCCCCTCCAG
TCATTTGGTCCACCCCTCATGCCATGGCAGTGGAGCTCAGGAGGTGCCATCCTCGAGGCC
TCAGGCTGCCCTGATCTGGAGTAGAGAAATCAATGGGCCACCCAGGGACCACTTGTCTTC
TTCACCATTGCTGGAAAGTTCCTTAAGTTCCACCATTCAGGTAGATAAAAATCAAGGTTC
CTTACCCTGTGGAGCAAAAGAACTAGACATTATGTCCAACACTCCACCTCCCCGCCCCCC
TAAGCCAAGCCATCTGTCTGAACGGCGCCAAGAGGAGTGGAGTACACACAGTGGTAGCAA
GAAGCCAGAATGCACTCTGGTTCCAAGAAGAATCTCCCTCTCTGGTTTAGACAACATGAG
AACCTGGAAAGCTGATGTAGAAGGCCAATCCTTAAGACACCGAGACAAGCGGCTTAGTTT
GAATTTGCCATGCAGGTTCTCCCCGATGTACCCCACAGCTTCAGCCAGTATCGAAGACAG
CTATGTGCCCATGAGCCCCCAGGCTGGTGCCTCTGGTCTTGGACCCCACTGCAGCCCTGA
TGACTACATTCCAATGAACTCAGGAAGCATCTCAAGCCCGTTGCCTGAGCTGCCTGCAAA
CCTGGAACCTCCCCCAGTGAATAGAGATCTCAAGCCTCAGAGGAAATCACGGCCACCTCC
TCTGGACCTGAGAAACCTCTCGATCATCCGGGAACATGCATCTCTTACCAGGACCCGCAC
TGTGCCTTGCAGTCGAACCAGCTTTCTCTCTCCAGAAAGAAATGGTATTAATTCTGCAAG
ATTTTTTGCTAATCCTGTTTCCAGAGAAGACGAAGAAAGCTACATCGAAATGGAGGAGCA
CCGAACAGCCAGTTCCCTGAGCAGTGGTGCCCTTACGTGGACAAAGAAATTCAGCCTAGA
TTATTTGGCCCTGGACTTCAATTCAGCATCACCAGCCCCCATGCAGCAGAAACTTCTCCT
TTCAGAAGAACAAAGAGTAGACTATGTCCAAGTGGATGAGCAGAAGACACAGGCTCTCCA
GAGCACAAAACAGGAGTGGACGGATGAAAGGCAATCCAAAGTAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT