Restriction map for
pFN21AB7259
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AlwI AlwNI AscI
Asp718I AviII AvrII BamHI BbrPI
BcgI BciVI BclI BglI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeIII MfeI MluI MroI
MspA1I MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 959 GGCGCC G[GA]^CG[CT]C
AocI 754 CCTCAGG CC^T[ACGT]AGG
ApaI 213 GGGCCC GGGCC^C
AseI 1462 ATTAAT AT^TAAT
AsnI 1462 ATTAAT AT^TAAT
Asp700I 243 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1675 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 959 GGCGCC GGCGC^C
BbvCI 183 CCTCAGC CCTCAGC(-5/-2)
BglII 1320 AGATCT A^GATCT
BsaAI 1569 TACGTG [CT]AC^GT[GA]
BsaBI 859 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 959 GGCGCC G[GA]^CG[CT]C
BsmFI 800 GGGAC GGGAC(10/14)
BsmI 1003 GAATGC GAATGC(1/-1)
BsrDI 821 CATTGC GCAATG(2/0)
BsrFI 25 ACCGGC [GA]^CCGG[CT]
BstAPI 306 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1320 AGATCT [GA]^GATC[CT]
Bsu36I 754 CCTCAGG CC^T[ACGT]AGG
Cfr10I 25 ACCGGC [GA]^CCGG[CT]
DraIII 980 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 532 CTCTTC CTCTTC(1/4)
Eco81I 754 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1391 ATGCAT ATGCA^T
HgaI 13 GACGC GACGC(5/10)
Hin1I 959 GGCGCC G[GA]^CG[CT]C
HincII 667 GTTGAC GT[CT]^[GA]AC
HindII 667 GTTGAC GT[CT]^[GA]AC
KasI 959 GGCGCC G^GCGCC
Ksp632I 532 CTCTTC CTCTTC(1/4)
MaeII 1570 ACGT A^CGT
MamI 859 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1320 AGATCT [GA]^GATC[CT]
NarI 959 GGCGCC GG^CGCC
NsiI 1391 ATGCAT ATGCA^T
PaeR7I 747 CTCGAG C^TCGAG
PflFI 1675 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 520 GAGTC GAGTC(4/5)
PpuMI 1405 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1462 ATTAAT AT^TAAT
SacI 728 GAGCTC GAGCT^C
ScaI 137 AGTACT AGT^ACT
SexAI 287 ACCTGGT A^CC[AT]GGT
SfoI 959 GGCGCC GGC^GCC
StuI 751 AGGCCT AGG^CCT
Tth111I 1675 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 357 CCACCTGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 747 CTCGAG C^TCGAG
XhoII 1320 AGATCT [GA]^GATC[CT]
XmnI 243 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 593 GTCTAC GT^[AC][GT]AC
1672 GTAGAC
AflII 837 CTTAAG C^TTAAG
1086 CTTAAG
AhdI 802 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
901 GACATTATGTC
Alw44I 21 GTGCAC G^TGCAC
325 GTGCAC
Aor51HI 65 AGCGCT AGC^GCT
83 AGCGCT
ApaLI 21 GTGCAC G^TGCAC
325 GTGCAC
AspEI 802 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
901 GACATTATGTC
AvaI 49 CCCGAG C^[CT]CG[GA]G
747 CTCGAG
BalI 293 TGGCCA TGG^CCA
354 TGGCCA
BcnI 171 CCGGG CC^[GC]GG
1383 CCGGG
BfaI 898 CTAG C^TAG
1591 CTAG
BfrI 837 CTTAAG C^TTAAG
1086 CTTAAG
BsmAI 568 GAGAC GTCTC(1/5)
1097 GAGAC
BsoBI 49 CCCGAG C^[CT]CG[GA]G
747 CTCGAG
BspMI 315 GCAGGT ACCTGC(4/8)
1127 GCAGGT
BsrBI 682 CCGCTC CCGCTC(-3/-3)
114 GAGCGG
BstXI 692 CCAGTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCATGGCAGTGG
DraII 213 GGGCCCC [GA]G^G[ACGT]CC[CT]
1405 AGGACCC
DsaI 380 CCATGG C^C[GA][CT]GG
717 CCATGG
Eam1105I 802 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
901 GACATTATGTC
Eco47III 65 AGCGCT AGC^GCT
83 AGCGCT
EcoO109I 213 GGGCCCC [GA]G^G[ACGT]CC[CT]
1405 AGGACCC
MaeI 898 CTAG C^TAG
1591 CTAG
MluNI 293 TGGCCA TGG^CCA
354 TGGCCA
MscI 293 TGGCCA TGG^CCA
354 TGGCCA
NciI 171 CCGGG CC^[GC]GG
1383 CCGGG
NcoI 380 CCATGG C^CATGG
717 CCATGG
NspI 207 GCATGT [GA]CATG^[CT]
1389 ACATGC
TfiI 376 GATTC G^A[AT]TC
1025 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I