(Circular) (Six-base) MAPSORT of: pFN21AB7259.seq Check: 929 from: 1 to: 6463 pFN21AB7259 6463 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 6463 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2599 3678 3806 1937 Size: 662 1079 128 4594 Fragments arranged by size: 4594 1079 662 128 Acc65I G'GTAC_C Cuts at: 3784 3784 Size: 6463 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3144 4526 5910 6178 1632 Size: 1512 1382 1384 268 1917 Fragments arranged by size: 1917 1512 1384 1382 268 AfeI AGC'GCT Cuts at: 2072 2090 2072 Size: 18 6445 AflII C'TTAA_G Cuts at: 820 1017 2842 3091 820 Size: 197 1825 249 4192 Fragments arranged by size: 4192 1825 249 197 AflIII A'CryG_T Cuts at: 1203 5362 6339 1203 Size: 4159 977 1327 Fragments arranged by size: 4159 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 2812 2911 5192 2812 Size: 99 2281 4083 Fragments arranged by size: 4083 2281 99 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6463 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 2144 Size: 32 6431 AlwNI CAG_nnn'CTG Cuts at: 4206 5778 4206 Size: 1572 4891 ApaI G_GGCC'C Cuts at: 2222 2222 Size: 6463 ApaLI G'TGCA_C Cuts at: 2026 2330 4520 5676 2026 Size: 304 2190 1156 2813 Fragments arranged by size: 2813 2190 1156 304 ApoI r'AATT_y Cuts at: 1493 2240 3121 3587 3772 3998 4150 6259 1493 Size: 747 881 466 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 881 747 466 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 3468 5017 160 Size: 872 2436 1549 1606 Fragments arranged by size: 2436 1606 1549 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2752 3788 1870 Size: 73 111 698 1036 4545 Fragments arranged by size: 4545 1036 698 111 73 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2743 2964 3206 3566 3784 Size: 325 217 627 956 221 242 360 218 Cuts at: 3784 5239 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 956 627 360 325 242 221 218 217 BanII G_rGCy'C Cuts at: 1391 2222 2737 3197 3782 1391 Size: 831 515 460 585 4072 Fragments arranged by size: 4072 831 585 515 460 BbeI G_GCGC'C Cuts at: 2968 2968 Size: 6463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2587 2808 3180 3513 3710 928 Size: 971 688 221 372 333 197 3681 Fragments arranged by size: 3681 971 688 372 333 221 197 BbvCI CC'TCA_GC Cuts at: 2189 2189 Size: 6463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4654 4688 1903 Size: 34 2717 34 3678 Fragments arranged by size: 3678 2717 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4355 5565 4355 Size: 1210 5253 BclI T'GATC_A Cuts at: 1548 1548 Size: 6463 BfrBI ATG'CAT Cuts at: 3398 3398 Size: 6463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5074 136 Size: 107 122 71 631 605 3402 1525 Fragments arranged by size: 3402 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3325 6458 3325 Size: 3133 3330 BlpI GC'TnA_GC Cuts at: 3880 3880 Size: 6463 Bme1580I G_kGCm'C Cuts at: 1790 2030 2222 2334 3189 3571 4524 5680 1790 Size: 240 192 112 855 382 953 1156 2573 Fragments arranged by size: 2573 1156 953 855 382 240 192 112 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3308 4194 5152 Size: 416 53 83 399 24 1891 886 958 Cuts at: 5152 442 Size: 1753 Fragments arranged by size: 1891 1753 958 886 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2413 2413 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2146 2387 2680 2797 3435 3701 5123 1239 Size: 907 241 293 117 638 266 1422 2579 Fragments arranged by size: 2579 1422 907 638 293 266 241 117 Bpu10I CC'TnA_GC Cuts at: 2189 2736 2940 3285 3557 2189 Size: 547 204 345 272 5095 Fragments arranged by size: 5095 547 345 272 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3256 3314 4588 5453 5751 3256 Size: 58 1274 865 298 3968 Fragments arranged by size: 3968 1274 865 298 58 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5126 882 Size: 617 3627 2219 Fragments arranged by size: 3627 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 4254 493 Size: 711 2372 678 2702 Fragments arranged by size: 2702 2372 711 678 BsaBI GATnn'nnATC Cuts at: 2868 6457 2868 Size: 3589 2874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2965 4270 4652 5287 Size: 53 83 186 936 1432 1305 382 635 Cuts at: 5287 275 Size: 1451 Fragments arranged by size: 1451 1432 1305 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 5294 5568 5715 6126 4896 Size: 398 274 147 411 5233 Fragments arranged by size: 5233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2393 2423 2911 Size: 30 165 30 741 30 219 30 488 Cuts at: 2911 2941 3699 3729 1178 Size: 30 758 30 3912 Fragments arranged by size: 3912 758 741 488 219 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2401 2455 2987 3348 3548 1186 Size: 1215 54 532 361 200 4101 Fragments arranged by size: 4101 1215 532 361 200 54 BseYI C'CCAG_C Cuts at: 2222 5666 6162 2222 Size: 3444 496 2523 Fragments arranged by size: 3444 2523 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2220 2397 2570 1163 Size: 573 484 177 173 5056 Fragments arranged by size: 5056 573 484 177 173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2334 2737 3540 3725 4524 4609 5680 2030 Size: 304 403 803 185 799 85 1071 2813 Fragments arranged by size: 2813 1071 803 799 403 304 185 85 BsmI GAATG_Cn' Cuts at: 3014 3974 4067 3014 Size: 960 93 5410 Fragments arranged by size: 5410 960 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2222 2334 2737 3189 3197 3540 Size: 399 240 192 112 403 452 8 343 Cuts at: 3540 3571 3725 3782 4524 4609 5680 1391 Size: 31 154 57 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 403 399 343 240 192 154 112 85 57 31 8 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 4352 6082 4352 Size: 1730 4733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 3123 3818 844 Size: 1467 812 695 3489 Fragments arranged by size: 3489 1467 812 695 BsrBI CCG'CTC Cuts at: 1394 1823 2121 2689 4350 1394 Size: 429 298 568 1661 3507 Fragments arranged by size: 3507 1661 568 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 2825 4958 5132 65 Size: 1183 1577 2133 174 1396 Fragments arranged by size: 2133 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 5107 5294 1700 Size: 110 220 3077 187 2869 Fragments arranged by size: 3077 2869 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 2317 794 Size: 1523 4940 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstXI CCAn_nnnn'nTGG Cuts at: 2704 2729 2704 Size: 25 6438 BstYI r'GATC_y Cuts at: 1972 3325 3793 4160 4545 4562 6014 6100 6112 Size: 1353 468 367 385 17 1452 86 12 Cuts at: 6112 6458 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1353 468 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2760 2760 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 2385 2722 513 Size: 552 1320 337 4254 Fragments arranged by size: 4254 1320 552 337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 2028 2208 2734 3226 3568 3979 4773 4793 2028 Size: 180 526 492 342 411 794 20 3698 Fragments arranged by size: 3698 794 526 492 411 342 180 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2957 2992 529 Size: 35 2393 35 4000 Fragments arranged by size: 4000 2393 35 35 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DraIII CAC_nnn'GTG Cuts at: 2990 2990 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2359 3350 Size: 54 1425 50 275 18 468 61 991 Cuts at: 3350 3910 4799 6428 8 Size: 560 889 1629 43 Fragments arranged by size: 1629 1425 991 889 560 468 275 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 4393 1353 Size: 1190 1850 3423 Fragments arranged by size: 3423 1850 1190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2735 2735 Size: 6463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2387 2680 2797 3144 3435 3701 Size: 393 514 241 293 117 347 291 266 Cuts at: 3701 4526 5123 5910 6178 1239 Size: 825 597 787 268 1524 Fragments arranged by size: 1524 825 787 597 514 393 347 293 291 268 266 241 117 EcoO109I rG'GnC_Cy Cuts at: 2219 3411 2219 Size: 1192 5271 EcoRI G'AATT_C Cuts at: 3772 4150 3772 Size: 378 6085 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 2074 2092 2968 5610 1678 Size: 396 18 876 2642 2531 Fragments arranged by size: 2642 2531 876 396 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2606 2638 2732 2764 2798 Size: 32 135 32 1034 32 94 32 34 Cuts at: 2798 2830 2897 2929 3697 3729 5104 5136 5178 Size: 32 67 32 768 32 1375 32 42 Cuts at: 5178 5210 1373 Size: 32 2626 Fragments arranged by size: 2626 1375 1034 768 135 94 67 42 34 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3807 4053 669 Size: 988 281 736 1133 246 3079 Fragments arranged by size: 3079 1133 988 736 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KasI G'GCGC_C Cuts at: 2964 2964 Size: 6463 KpnI G_GTAC'C Cuts at: 3788 3788 Size: 6463 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2589 2940 5577 5761 1305 Size: 1284 351 2637 184 2007 Fragments arranged by size: 2637 2007 1284 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2300 2361 10 Size: 54 1425 343 468 61 4112 Fragments arranged by size: 4112 1425 468 343 61 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2727 3251 3527 4421 4780 4939 Size: 648 1011 550 524 276 894 359 159 Cuts at: 4939 518 Size: 2042 Fragments arranged by size: 2042 1011 894 648 550 524 359 276 159 MspA1I CmG'CkG Cuts at: 1606 3879 4556 5704 5949 1606 Size: 2273 677 1148 245 2120 Fragments arranged by size: 2273 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2965 2965 Size: 6463 NcoI C'CATG_G Cuts at: 513 1065 2385 2722 513 Size: 552 1320 337 4254 Fragments arranged by size: 4254 1320 552 337 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NsiI A_TGCA'T Cuts at: 3400 3400 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2216 3398 3821 5366 1132 Size: 97 987 1182 423 1545 2229 Fragments arranged by size: 2229 1545 1182 987 423 97 PasI CC'CwG_GG Cuts at: 1348 2802 1348 Size: 1454 5009 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6463 PfoI T'CCnGG_A Cuts at: 3387 3387 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2176 4532 4564 6045 6077 2144 Size: 32 2356 32 1481 32 2530 Fragments arranged by size: 2530 2356 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3411 3411 Size: 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 4033 4033 Size: 6463 PspOMI G'GGCC_C Cuts at: 2218 2218 Size: 6463 PspXI vC'TCGA_Gb Cuts at: 2752 2752 Size: 6463 PstI C_TGCA'G Cuts at: 830 2421 2685 3233 3815 830 Size: 1591 264 548 582 3478 Fragments arranged by size: 3478 1591 582 548 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 SacI G_AGCT'C Cuts at: 2737 2737 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 2144 4711 6423 2144 Size: 2567 1712 2184 Fragments arranged by size: 2567 2184 1712 SexAI A'CCwGG_T Cuts at: 2292 2292 Size: 6463 SfcI C'TryA_G Cuts at: 826 1045 2066 2146 2417 2681 3229 3811 4946 Size: 219 1021 80 271 264 548 582 1135 Cuts at: 4946 5627 5818 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1021 681 582 548 271 264 219 191 80 SfoI GGC'GCC Cuts at: 2966 2966 Size: 6463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 2842 3091 3271 3329 4567 Size: 197 926 809 90 249 180 58 1238 Cuts at: 4567 5468 5730 820 Size: 901 262 1553 Fragments arranged by size: 1553 1238 926 901 809 262 249 197 180 90 58 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 3821 1132 Size: 2689 3774 SspI AAT'ATT Cuts at: 5 52 4387 5 Size: 47 4335 2081 Fragments arranged by size: 4335 2081 47 StuI AGG'CCT Cuts at: 1517 2758 1517 Size: 1241 5222 StyI C'CwwG_G Cuts at: 513 1065 2356 2385 2722 3902 513 Size: 552 1291 29 337 1180 3074 Fragments arranged by size: 3074 1291 1180 552 337 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2317 2815 4490 4507 4660 4845 5290 Size: 1255 737 498 1675 17 153 185 445 Cuts at: 5290 325 Size: 1498 Fragments arranged by size: 1675 1498 1255 737 498 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 2981 4709 Size: 274 80 33 51 1443 165 839 1728 Cuts at: 4709 6421 96 Size: 1712 138 Fragments arranged by size: 1728 1712 1443 839 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2911 2943 3004 3036 3699 3731 6423 6455 2911 Size: 32 61 32 663 32 2692 32 2919 Fragments arranged by size: 2919 2692 663 61 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3683 1316 Size: 2367 4096 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2369 2369 Size: 6463 XhoI C'TCGA_G Cuts at: 1943 2752 1943 Size: 809 5654 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 2252 4592 2252 Size: 2340 4123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI