ZNF250 ORF clone FHC01237

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01237

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Clone info.

Species

human

Clone Name

pFN21AB3061 Sequence (with vector) Restriction Map

Product ID

FHC01237

Flexi Clone PID

FXC01237

ORF Sequence

DNA sequence (1665 bp)
Protein sequence (555 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384739]

Gene

KIBB3061

Symbol

ZNF250

Alias : ZFP647, ZNF647

Description

zinc finger protein 250, transcript variant 2

Original Clone ID

hg04778

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1665 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 555 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55282.1 7e-127 100.0 ZNF250 CCDS34972.1 3.3e-126 99.1 ZNF250 CCDS74472.1 1.8e-55 53.6 ZNF135 CCDS74471.1 1.9e-55 53.6 ZNF135 CCDS12970.2 1.9e-55 53.6 ZNF135 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001909 17 57 PF01352 Krueppel-associated box IPR007087 194 216 PF00096 Zinc finger IPR007087 222 244 PF00096 Zinc finger IPR007087 306 328 PF00096 Zinc finger IPR007087 334 356 PF00096 Zinc finger IPR007087 362 384 PF00096 Zinc finger IPR007087 390 412 PF00096 Zinc finger IPR007087 418 440 PF00096 Zinc finger IPR007087 474 496 PF00096 Zinc finger IPR007087 502 524 PF00096 Zinc finger IPR007087 530 552 PF00096 Zinc finger HMMSmart IPR001909 17 77 SM00349 Krueppel-associated box IPR015880 194 216 SM00355 Zinc finger IPR015880 222 244 SM00355 Zinc finger IPR015880 250 272 SM00355 Zinc finger IPR015880 278 300 SM00355 Zinc finger IPR015880 306 328 SM00355 Zinc finger IPR015880 334 356 SM00355 Zinc finger IPR015880 362 384 SM00355 Zinc finger IPR015880 390 412 SM00355 Zinc finger IPR015880 418 440 SM00355 Zinc finger IPR015880 446 468 SM00355 Zinc finger IPR015880 474 496 SM00355 Zinc finger IPR015880 502 524 SM00355 Zinc finger IPR015880 530 552 SM00355 Zinc finger ProfileScan IPR001909 17 88 PS50805 Krueppel-associated box IPR007087 194 221 PS50157 Zinc finger IPR007087 222 249 PS50157 Zinc finger IPR007087 250 277 PS50157 Zinc finger IPR007087 278 305 PS50157 Zinc finger IPR007087 306 333 PS50157 Zinc finger IPR007087 334 361 PS50157 Zinc finger IPR007087 362 389 PS50157 Zinc finger IPR007087 390 417 PS50157 Zinc finger IPR007087 418 445 PS50157 Zinc finger IPR007087 446 473 PS50157 Zinc finger IPR007087 474 501 PS50157 Zinc finger IPR007087 502 529 PS50157 Zinc finger IPR007087 530 555 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001276331 616 604077 53.6 83.8 XP_016882731 621 604077 52.9 85.9 XP_016882730 628 604077 53.6 83.8 XP_016882729 658 604077 53.6 83.8 NP_001276330 658 604077 53.6 83.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3061 Download

>KIBB3061  1665 bp
ATGGCAGCAGCCAGACTCCTGCCAGTGCCGGCAGGACCCCAGGCCAAGCTGACCTTCGAG
GATGTGGCTGTGCTCCTCTCCCAGGATGAATGGGACCGCCTGTGCCCTGCTCAGAGGGGC
CTCTACAGAAATGTGATGATGGAAACCTATGGGAATGTAGTCTCATTGGGACTTCCAGGA
TCCAAGCCTGACATAATCTCCCAGCTGGAGCGAGGGGAAGATCCCTGGGTCCTGGACAGG
AAGGGGGCTAAGAAGAGCCAGGGCCTGTGGAGTGACTACTCAGACAACCTCAAATATGAC
CACACTACAGCCTGTACACAACAAGACAGTTTATCTTGTCCATGGGAATGTGAAACCAAG
GGAGAGAGTCAAAATACAGACTTGAGTCCGAAGCCATTAATTTCAGAGCAAACAGTGATT
CTGGGGAAAACACCCTTGGGGAGGATTGATCAAGAAAATAATGAAACAAAGCAAAGCTTC
TGTCTGAGTCCAAACTCTGTTGACCACCGTGAAGTTCAGGTCTTAAGCCAAAGCATGCCA
CTCACTCCGCACCAGGCAGTGCCTAGTGGAGAGAGGCCCTACATGTGTGTTGAGTGTGGG
AAGTGCTTTGGCCGGAGTTCCCACCTCCTTCAGCATCAGCGTATCCACACTGGAGAGAAG
CCCTATGTGTGCAGTGTATGTGGGAAGGCCTTCAGCCAGAGCTCAGTCCTTAGTAAACAC
AGGAGAATTCACACAGGTGAGAAGCCCTATGAGTGTAATGAGTGTGGAAAAGCCTTTAGA
GTGAGCTCAGATCTTGCTCAGCATCACAAGATACATACAGGAGAGAAGCCTCACGAATGT
CTTGAGTGTCGGAAAGCCTTCACTCAACTCTCACATCTCATTCAGCACCAGCGGATCCAC
ACGGGAGAAAGGCCATATGTGTGTCCGTTGTGTGGGAAAGCCTTCAACCATAGCACTGTT
CTGCGGAGCCACCAGAGGGTACACACTGGGGAGAAGCCTCACAGGTGCAATGAGTGTGGG
AAAACCTTCAGTGTGAAGAGGACACTGCTGCAGCACCAGAGGATCCACACCGGGGAGAAG
CCCTACACGTGCAGCGAGTGTGGGAAGGCCTTCAGCGACCGCTCAGTCCTCATTCAGCAC
CACAACGTGCACACCGGGGAGAAGCCCTATGAGTGCAGTGAGTGTGGGAAGACCTTCAGC
CACCGCTCCACACTGATGAATCACGAGCGGATCCACACCGAGGAAAAGCCCTATGCATGC
TACGAATGTGGGAAGGCCTTCGTTCAGCACTCACACCTGATCCAGCACCAGAGAGTCCAC
ACTGGGGAGAAGCCCTATGTGTGTGGTGAATGTGGGCACGCCTTCAGTGCACGCCGGTCT
CTGATCCAGCATGAGAGAATCCACACAGGTGAAAAGCCCTTCCAGTGCACAGAATGTGGC
AAAGCCTTCAGCCTGAAAGCAACTCTGATTGTGCACCTGAGGACCCACACGGGCGAGAAG
CCATATGAGTGCAATAGCTGCGGGAAGGCCTTCAGCCAGTACTCAGTGCTCATCCAGCAC
CAGCGGATCCACACAGGCGAGAAGCCCTATGAGTGCGGGGAGTGTGGGCGTGCCTTCAAC
CAGCATGGCCACCTAATCCAGCACCAGAAAGTGCACAGAAAGTTG

ORF Protein

Cloned ORF protein sequence for pFN21AB3061 Download

>KIBB3061  555 aa
MAAARLLPVPAGPQAKLTFEDVAVLLSQDEWDRLCPAQRGLYRNVMMETYGNVVSLGLPG
SKPDIISQLERGEDPWVLDRKGAKKSQGLWSDYSDNLKYDHTTACTQQDSLSCPWECETK
GESQNTDLSPKPLISEQTVILGKTPLGRIDQENNETKQSFCLSPNSVDHREVQVLSQSMP
LTPHQAVPSGERPYMCVECGKCFGRSSHLLQHQRIHTGEKPYVCSVCGKAFSQSSVLSKH
RRIHTGEKPYECNECGKAFRVSSDLAQHHKIHTGEKPHECLECRKAFTQLSHLIQHQRIH
TGERPYVCPLCGKAFNHSTVLRSHQRVHTGEKPHRCNECGKTFSVKRTLLQHQRIHTGEK
PYTCSECGKAFSDRSVLIQHHNVHTGEKPYECSECGKTFSHRSTLMNHERIHTEEKPYAC
YECGKAFVQHSHLIQHQRVHTGEKPYVCGECGHAFSARRSLIQHERIHTGEKPFQCTECG
KAFSLKATLIVHLRTHTGEKPYECNSCGKAFSQYSVLIQHQRIHTGEKPYECGECGRAFN
QHGHLIQHQKVHRKL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3061 Download

>pFN21AB3061  6370 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGCAGCCAGACTCCTGCCAGTGCCGGCAGG
ACCCCAGGCCAAGCTGACCTTCGAGGATGTGGCTGTGCTCCTCTCCCAGGATGAATGGGA
CCGCCTGTGCCCTGCTCAGAGGGGCCTCTACAGAAATGTGATGATGGAAACCTATGGGAA
TGTAGTCTCATTGGGACTTCCAGGATCCAAGCCTGACATAATCTCCCAGCTGGAGCGAGG
GGAAGATCCCTGGGTCCTGGACAGGAAGGGGGCTAAGAAGAGCCAGGGCCTGTGGAGTGA
CTACTCAGACAACCTCAAATATGACCACACTACAGCCTGTACACAACAAGACAGTTTATC
TTGTCCATGGGAATGTGAAACCAAGGGAGAGAGTCAAAATACAGACTTGAGTCCGAAGCC
ATTAATTTCAGAGCAAACAGTGATTCTGGGGAAAACACCCTTGGGGAGGATTGATCAAGA
AAATAATGAAACAAAGCAAAGCTTCTGTCTGAGTCCAAACTCTGTTGACCACCGTGAAGT
TCAGGTCTTAAGCCAAAGCATGCCACTCACTCCGCACCAGGCAGTGCCTAGTGGAGAGAG
GCCCTACATGTGTGTTGAGTGTGGGAAGTGCTTTGGCCGGAGTTCCCACCTCCTTCAGCA
TCAGCGTATCCACACTGGAGAGAAGCCCTATGTGTGCAGTGTATGTGGGAAGGCCTTCAG
CCAGAGCTCAGTCCTTAGTAAACACAGGAGAATTCACACAGGTGAGAAGCCCTATGAGTG
TAATGAGTGTGGAAAAGCCTTTAGAGTGAGCTCAGATCTTGCTCAGCATCACAAGATACA
TACAGGAGAGAAGCCTCACGAATGTCTTGAGTGTCGGAAAGCCTTCACTCAACTCTCACA
TCTCATTCAGCACCAGCGGATCCACACGGGAGAAAGGCCATATGTGTGTCCGTTGTGTGG
GAAAGCCTTCAACCATAGCACTGTTCTGCGGAGCCACCAGAGGGTACACACTGGGGAGAA
GCCTCACAGGTGCAATGAGTGTGGGAAAACCTTCAGTGTGAAGAGGACACTGCTGCAGCA
CCAGAGGATCCACACCGGGGAGAAGCCCTACACGTGCAGCGAGTGTGGGAAGGCCTTCAG
CGACCGCTCAGTCCTCATTCAGCACCACAACGTGCACACCGGGGAGAAGCCCTATGAGTG
CAGTGAGTGTGGGAAGACCTTCAGCCACCGCTCCACACTGATGAATCACGAGCGGATCCA
CACCGAGGAAAAGCCCTATGCATGCTACGAATGTGGGAAGGCCTTCGTTCAGCACTCACA
CCTGATCCAGCACCAGAGAGTCCACACTGGGGAGAAGCCCTATGTGTGTGGTGAATGTGG
GCACGCCTTCAGTGCACGCCGGTCTCTGATCCAGCATGAGAGAATCCACACAGGTGAAAA
GCCCTTCCAGTGCACAGAATGTGGCAAAGCCTTCAGCCTGAAAGCAACTCTGATTGTGCA
CCTGAGGACCCACACGGGCGAGAAGCCATATGAGTGCAATAGCTGCGGGAAGGCCTTCAG
CCAGTACTCAGTGCTCATCCAGCACCAGCGGATCCACACAGGCGAGAAGCCCTATGAGTG
CGGGGAGTGTGGGCGTGCCTTCAACCAGCATGGCCACCTAATCCAGCACCAGAAAGTGCA
CAGAAAGTTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT