(Circular) (Six-base) MAPSORT of: pFN21AB3061.seq Check: 2677 from: 1 to: 6370 pFN21AB3061 6370 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 2680 3016 3100 3184 3352 3436 3520 Size: 986 62 336 84 84 168 84 84 Cuts at: 3520 4433 5817 6085 1632 Size: 913 1384 268 1917 Fragments arranged by size: 1917 1384 986 913 336 268 168 84 84 84 84 62 AflII C'TTAA_G Cuts at: 820 1017 2527 820 Size: 197 1510 4663 Fragments arranged by size: 4663 1510 197 AflIII A'CryG_T Cuts at: 1203 2586 3090 5269 6246 1203 Size: 1383 504 2179 977 1327 Fragments arranged by size: 2179 1383 1327 977 504 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 2739 3411 1166 Size: 1573 672 4125 Fragments arranged by size: 4125 1573 672 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2981 1184 Size: 1797 4573 AlwNI CAG_nnn'CTG Cuts at: 2269 4113 5685 2269 Size: 1844 1572 2954 Fragments arranged by size: 2954 1844 1572 ApaLI G'TGCA_C Cuts at: 3152 3372 3430 3476 3656 4427 5583 3152 Size: 220 58 46 180 771 1156 3939 Fragments arranged by size: 3939 1156 771 220 180 58 46 ApoI r'AATT_y Cuts at: 1493 2730 3679 3905 4057 6166 1493 Size: 1237 949 226 152 2109 1697 Fragments arranged by size: 2109 1697 1237 949 226 152 AseI AT'TA_AT Cuts at: 160 1032 2402 4924 160 Size: 872 1370 2522 1606 Fragments arranged by size: 2522 1606 1370 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 3695 1870 Size: 73 1752 4545 Fragments arranged by size: 4545 1752 73 BamHI G'GATC_C Cuts at: 2183 2898 3066 3234 3570 3700 4067 2183 Size: 715 168 168 336 130 367 4486 Fragments arranged by size: 4486 715 367 336 168 168 130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3691 5146 618 Size: 325 217 627 1904 1455 1842 Fragments arranged by size: 1904 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2708 2792 3689 1391 Size: 1317 84 897 4072 Fragments arranged by size: 4072 1317 897 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3200 928 Size: 971 1301 4098 Fragments arranged by size: 4098 1301 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2657 4262 5472 2657 Size: 1605 1210 3555 Fragments arranged by size: 3555 1605 1210 BclI T'GATC_A Cuts at: 1548 2452 1548 Size: 904 5466 BfrBI ATG'CAT Cuts at: 3260 3260 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4981 136 Size: 107 122 71 631 605 3309 1525 Fragments arranged by size: 3309 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2794 6365 2794 Size: 3571 2799 BlpI GC'TnA_GC Cuts at: 2802 3787 2802 Size: 985 5385 Bme1580I G_kGCm'C Cuts at: 1790 2111 3156 3363 3376 3434 3480 3660 4431 Size: 321 1045 207 13 58 46 180 771 Cuts at: 4431 5587 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1045 771 321 207 180 58 46 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3000 3336 4101 Size: 416 53 83 399 24 1583 336 765 Cuts at: 4101 5059 442 Size: 958 1753 Fragments arranged by size: 1753 1583 958 765 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2676 5030 1239 Size: 992 445 2354 2579 Fragments arranged by size: 2579 2354 992 445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2407 2867 4495 5360 5658 2407 Size: 460 1628 865 298 3119 Fragments arranged by size: 3119 1628 865 460 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3387 5033 882 Size: 617 1888 1646 2219 Fragments arranged by size: 2219 1888 1646 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 4161 493 Size: 711 1889 1068 2702 Fragments arranged by size: 2702 1889 1068 711 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4177 4559 5194 275 Size: 53 83 186 936 2644 382 635 1451 Fragments arranged by size: 2644 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4803 5201 5475 5622 6033 4803 Size: 398 274 147 411 5140 Fragments arranged by size: 5140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 5332 Fragments arranged by size: 5332 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 1186 Size: 883 5487 BseYI C'CCAG_C Cuts at: 2205 5573 6069 2205 Size: 3368 496 2506 Fragments arranged by size: 3368 2506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2695 3115 3199 1163 Size: 573 959 420 84 4334 Fragments arranged by size: 4334 959 573 420 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3820 4581 5609 1540 Size: 275 186 1123 696 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 696 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2708 2792 3156 3376 3434 3480 3555 3660 Size: 629 84 364 220 58 46 75 105 Cuts at: 3660 4431 4516 5587 2079 Size: 771 85 1071 2862 Fragments arranged by size: 2862 1071 771 629 364 220 105 85 84 75 58 46 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2111 2708 2792 3156 3363 3376 Size: 399 289 32 597 84 364 207 13 Cuts at: 3376 3434 3480 3555 3660 3689 4431 4516 5587 Size: 58 46 75 105 29 742 85 1071 Cuts at: 5587 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 597 399 364 289 207 105 85 84 75 58 46 32 29 13 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3725 844 Size: 2881 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3126 3210 3232 4257 1394 Size: 429 1303 84 22 1025 3507 Fragments arranged by size: 3507 1303 1025 429 84 22 BsrDI GCAATG_nn' Cuts at: 65 1248 3019 4865 5039 65 Size: 1183 1771 1846 174 1396 Fragments arranged by size: 1846 1771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 3378 5014 5201 1700 Size: 110 222 1346 1636 187 2869 Fragments arranged by size: 2869 1636 1346 222 187 110 BsrGI T'GTAC_A Cuts at: 96 2318 96 Size: 2222 4148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 3227 4430 5442 3227 Size: 1203 1012 4155 Fragments arranged by size: 4155 1203 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3522 794 Size: 2728 3642 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstYI r'GATC_y Cuts at: 1972 2183 2224 2794 2898 3066 3234 3570 3700 Size: 211 41 570 104 168 168 336 130 Cuts at: 3700 4067 4452 4469 5921 6007 6019 6365 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 346 336 211 168 168 130 104 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 3482 3482 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2004 2345 513 Size: 552 939 341 4538 Fragments arranged by size: 4538 939 552 341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 2568 2683 3047 3187 3886 4680 4700 2568 Size: 115 364 140 699 794 20 4238 Fragments arranged by size: 4238 794 699 364 140 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3001 3036 529 Size: 35 2437 35 3863 Fragments arranged by size: 3863 2437 35 35 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DraIII CAC_nnn'GTG Cuts at: 3151 3151 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3128 5377 809 Size: 507 1812 2249 1802 Fragments arranged by size: 2249 1812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2614 3631 3817 Size: 54 1425 50 275 18 784 1017 186 Cuts at: 3817 4706 6335 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 784 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3817 1537 Size: 275 2005 4090 Fragments arranged by size: 4090 2005 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 3035 4300 1353 Size: 899 783 1265 3423 Fragments arranged by size: 3423 1265 899 783 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4957 5331 5477 387 Size: 1064 138 3368 374 146 1280 Fragments arranged by size: 3368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2706 2790 2706 Size: 84 6286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2618 2676 2680 3016 3100 3184 Size: 393 599 387 58 4 336 84 84 Cuts at: 3184 3352 3436 3520 4433 5030 5817 6085 1239 Size: 168 84 84 913 597 787 268 1524 Fragments arranged by size: 1524 913 787 599 597 393 387 336 268 168 84 84 84 84 58 4 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2443 2084 Size: 359 6011 EcoO109I rG'GnC_Cy Cuts at: 2039 2122 2233 2266 2580 3486 2039 Size: 83 111 33 314 906 4923 Fragments arranged by size: 4923 906 314 111 83 33 EcoRI G'AATT_C Cuts at: 2730 3679 4057 2730 Size: 949 378 5043 Fragments arranged by size: 5043 949 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2074 2497 2074 Size: 423 5947 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 5517 1678 Size: 3839 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2321 2353 2437 Size: 32 135 32 614 32 103 32 84 Cuts at: 2437 2469 2779 2811 5011 5043 5085 5117 1373 Size: 32 310 32 2200 32 42 32 2626 Fragments arranged by size: 2626 2200 614 310 135 103 84 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3714 3960 669 Size: 988 281 568 1208 246 3079 Fragments arranged by size: 3079 1208 988 568 281 246 HindIII A'AGCT_T Cuts at: 748 2479 748 Size: 1731 4639 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2834 5484 5668 1305 Size: 1529 2650 184 2007 Fragments arranged by size: 2650 2007 1529 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3633 10 Size: 54 1425 343 1801 2747 Fragments arranged by size: 2747 1801 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2350 2739 2923 3218 3411 3511 4328 Size: 648 1184 389 184 295 193 100 817 Cuts at: 4328 4687 4846 518 Size: 359 159 2042 Fragments arranged by size: 2042 1184 817 648 389 359 295 193 184 159 100 MspA1I CmG'CkG Cuts at: 1606 2209 2896 3568 3786 4463 5611 5856 1606 Size: 603 687 672 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 672 603 245 218 NaeI GCC'GGC Cuts at: 2034 2034 Size: 6370 NcoI C'CATG_G Cuts at: 513 1065 2004 2345 513 Size: 552 939 341 4538 Fragments arranged by size: 4538 939 552 341 NdeI CA'TA_TG Cuts at: 387 2920 3508 387 Size: 2533 588 3249 Fragments arranged by size: 3249 2533 588 NgoMIV G'CCGG_C Cuts at: 2032 2032 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NsiI A_TGCA'T Cuts at: 3262 3262 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2542 2590 3264 3728 5273 1132 Size: 97 1313 48 674 464 1545 2229 Fragments arranged by size: 2229 1545 1313 674 464 97 48 PasI CC'CwG_GG Cuts at: 1348 2229 1348 Size: 881 5489 PciI A'CATG_T Cuts at: 2586 5269 2586 Size: 2683 3687 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PfoI T'CCnGG_A Cuts at: 2179 2235 2179 Size: 56 6314 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PmlI CAC'GTG Cuts at: 3093 3093 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2039 2233 3486 2039 Size: 194 1253 4923 Fragments arranged by size: 4923 1253 194 PshAI GACnn'nnGTC Cuts at: 1613 2388 1613 Size: 775 5595 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 3057 3722 830 Size: 2227 665 3478 Fragments arranged by size: 3478 2227 665 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2209 2209 Size: 6370 SacI G_AGCT'C Cuts at: 2708 2792 2708 Size: 84 6286 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SapI GCTCTTCn'nnn_ Cuts at: 2252 2252 Size: 6370 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 3545 4618 6330 3545 Size: 1073 1712 3585 Fragments arranged by size: 3585 1712 1073 SfcI C'TryA_G Cuts at: 826 1045 2128 2310 3053 3718 4853 5534 5725 Size: 219 1083 182 743 665 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1135 1083 743 681 665 219 191 182 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2386 2527 2846 4474 5375 5637 Size: 197 926 443 141 319 1628 901 262 Cuts at: 5637 820 Size: 1553 Fragments arranged by size: 1628 1553 926 901 443 319 262 197 141 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 2542 3264 3728 1132 Size: 1410 722 464 3774 Fragments arranged by size: 3774 1410 722 464 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2693 3113 3281 3533 1517 Size: 1176 420 168 252 4354 Fragments arranged by size: 4354 1176 420 252 168 StyI C'CwwG_G Cuts at: 513 1065 2004 2345 2361 2439 3809 513 Size: 552 939 341 16 78 1370 3074 Fragments arranged by size: 3074 1370 939 552 341 78 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4397 4414 4567 4752 5197 325 Size: 1255 2817 17 153 185 445 1498 Fragments arranged by size: 2817 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2318 3543 4616 Size: 274 80 33 51 1443 341 1225 1073 Cuts at: 4616 6328 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1225 1073 341 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 3288 3320 6330 6362 2090 Size: 32 1166 32 3010 32 2098 Fragments arranged by size: 3010 2098 1166 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 2693 2861 2945 3029 3113 3197 3281 3533 4499 Size: 168 84 84 84 84 84 252 966 Cuts at: 4499 2693 Size: 4564 Fragments arranged by size: 4564 966 252 168 84 84 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI