SLC9A8 ORF clone FHC00690

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Halotag ORF Clone FHC00690

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Clone info.

Species

human

Clone Name

pFN21ASDA0939 Sequence (with vector) Restriction Map

Product ID

FHC00690

Flexi Clone PID

FXC00690

ORF Sequence

DNA sequence (1743 bp)
Protein sequence (581 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384051]

Gene

KIAA0939

Symbol

SLC9A8

Alias : NHE-8, NHE8

Description

solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8, transcript variant 2

Original Clone ID

hh04825s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1743 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 581 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13421.1 0 100.0 SLC9A8 CCDS58774.1 2.5e-171 97.3 SLC9A8 CCDS33872.1 1.7e-56 38.3 SLC9A9 CCDS75967.1 3.1e-55 40.5 SLC9A7 CCDS14269.1 1.1e-53 40.3 SLC9A7 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR004709 125 136 PR01084 Na+/H+ exchanger IPR004709 139 153 PR01084 Na+/H+ exchanger IPR004709 154 162 PR01084 Na+/H+ exchanger IPR004709 194 204 PR01084 Na+/H+ exchanger HMMPfam IPR006153 68 472 PF00999 Cation/H+ exchanger HMMTigr IPR004709 63 490 TIGR00840 Na+/H+ exchanger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 16 HEGFNVTLHTTLVVTTKLVLPTP 38 SECONDARY 23 2 60 MTIFFSLLVLAICIILVHLLIR 81 PRIMARY 22 3 88 PESVAVVSLGILMGAVIKIIEF 109 PRIMARY 22 4 152 GSITLFAVFGTAISAFVVGGGIY 174 PRIMARY 23 5 187 MTDSFAFGSLISAVDPVATIAIF 209 SECONDARY 23 6 264 FLKMFFGSAALGTLTGLISALVL 286 SECONDARY 23 7 314 EGISLSGIMAILFSGIVMSHYTH 336 PRIMARY 23 8 352 RTVAFLCETCVFAFLGLSIFSFP 374 PRIMARY 23 9 381 FVIWCIVLVLFGRAVNIFPLSYL 403 PRIMARY 23 10 451 TTIVIVLFTILLLGGSTMPLIRL 473 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056081 581 612730 100.0 100.0 XP_011527041 589 612730 90.8 100.0 NP_001247420 597 612730 97.3 100.0 XP_006723819 614 612730 94.5 99.8 XP_011527039 602 612730 87.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0939 Download

>KIAA0939  1743 bp
ATGGGGGAGAAGATGGCGGAAGAGGAGAGGTTCCCCAATACAACTCATGAGGGTTTCAAT
GTCACCCTCCACACCACCCTGGTTGTCACGACGAAACTGGTGCTCCCGACCCCTGGCAAG
CCCATCCTCCCCGTGCAGACAGGGGAGCAGGCCCAGCAAGAGGAGCAGTCCAGCGGCATG
ACCATTTTCTTCAGCCTCCTTGTCCTAGCTATCTGCATCATATTGGTGCATTTACTGATC
CGATACAGATTACATTTCTTGCCAGAGAGTGTTGCTGTTGTTTCTTTAGGTATTCTCATG
GGAGCAGTTATAAAAATTATAGAGTTTAAAAAACTGGCGAATTGGAAGGAAGAAGAAATG
TTTCGTCCAAACATGTTTTTCCTCCTCCTGCTTCCCCCTATTATCTTTGAGTCTGGATAT
TCATTACACAAGGGTAACTTCTTTCAAAATATTGGTTCCATCACCCTGTTTGCTGTTTTT
GGGACGGCAATCTCCGCTTTTGTAGTAGGTGGAGGAATTTATTTTCTGGGTCAGGCTGAT
GTAATCTCTAAACTCAACATGACAGACAGTTTTGCGTTTGGCTCCCTAATATCTGCTGTC
GATCCAGTGGCCACTATTGCCATTTTCAATGCACTTCATGTGGACCCCGTGCTCAACATG
CTGGTCTTTGGAGAAAGTATTCTCAACGATGCAGTCTCCATTGTTCTGACCAACACAGCT
GAAGGTTTAACAAGAAAAAATATGTCAGATGTCAGTGGGTGGCAAACATTTTTACAAGCC
CTTGACTACTTCCTCAAAATGTTCTTTGGCTCTGCAGCGCTCGGCACTCTCACTGGCTTA
ATTTCTGCATTAGTGCTGAAGCATATTGACTTGAGGAAAACGCCTTCCTTGGAGTTTGGC
ATGATGATCATTTTTGCTTATCTGCCTTATGGGCTTGCAGAAGGAATCTCACTCTCAGGC
ATCATGGCCATCCTGTTCTCAGGCATCGTGATGTCCCACTACACGCACCATAACCTCTCC
CCAGTCACCCAGATCCTCATGCAGCAGACCCTCCGCACCGTGGCCTTCTTATGTGAAACA
TGTGTGTTTGCATTTCTTGGCCTGTCCATTTTTAGTTTTCCTCACAAGTTTGAAATTTCC
TTTGTCATCTGGTGCATAGTGCTTGTACTATTTGGCAGAGCGGTAAACATTTTCCCTCTT
TCCTACCTCCTGAATTTCTTCCGGGATCATAAAATCACACCGAAGATGATGTTCATCATG
TGGTTTAGTGGCCTGCGGGGAGCCATCCCCTATGCCCTGAGCCTACACCTGGACCTGGAG
CCCATGGAGAAGCGGCAGCTCATCGGCACCACCACCATCGTCATCGTGCTCTTCACCATC
CTGCTGCTGGGCGGCAGCACCATGCCCCTCATTCGCCTCATGGACATCGAGGACGCCAAG
GCACACCGCAGGAACAAGAAGGACGTCAACCTCAGCAAGACTGAGAAGATGGGCAACACT
GTGGAGTCGGAGCACCTGTCGGAGCTCACGGAGGAGGAGTACGAGGCCCACTACATCAGG
CGGCAGGACCTTAAGGGCTTCGTGTGGCTGGACGCCAAGTACCTGAACCCCTTCTTCACT
CGGAGGCTGACGCAGGAGGACCTGCACCACGGGCGCATCCAGATGAAAACTCTCACCAAC
AAGTGGTACGAGGAGGTACGCCAGGGCCCCTCCGGCTCCGAGGACGACGAGCAGGAGCTG
CTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0939 Download

>KIAA0939  581 aa
MGEKMAEEERFPNTTHEGFNVTLHTTLVVTTKLVLPTPGKPILPVQTGEQAQQEEQSSGM
TIFFSLLVLAICIILVHLLIRYRLHFLPESVAVVSLGILMGAVIKIIEFKKLANWKEEEM
FRPNMFFLLLLPPIIFESGYSLHKGNFFQNIGSITLFAVFGTAISAFVVGGGIYFLGQAD
VISKLNMTDSFAFGSLISAVDPVATIAIFNALHVDPVLNMLVFGESILNDAVSIVLTNTA
EGLTRKNMSDVSGWQTFLQALDYFLKMFFGSAALGTLTGLISALVLKHIDLRKTPSLEFG
MMIIFAYLPYGLAEGISLSGIMAILFSGIVMSHYTHHNLSPVTQILMQQTLRTVAFLCET
CVFAFLGLSIFSFPHKFEISFVIWCIVLVLFGRAVNIFPLSYLLNFFRDHKITPKMMFIM
WFSGLRGAIPYALSLHLDLEPMEKRQLIGTTTIVIVLFTILLLGGSTMPLIRLMDIEDAK
AHRRNKKDVNLSKTEKMGNTVESEHLSELTEEEYEAHYIRRQDLKGFVWLDAKYLNPFFT
RRLTQEDLHHGRIQMKTLTNKWYEEVRQGPSGSEDDEQELL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0939 Download

>pFN21ASDA0939  6472 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGGGGAGAAGATGGC
GGAAGAGGAGAGGTTCCCCAATACAACTCATGAGGGTTTCAATGTCACCCTCCACACCAC
CCTGGTTGTCACGACGAAACTGGTGCTCCCGACCCCTGGCAAGCCCATCCTCCCCGTGCA
GACAGGGGAGCAGGCCCAGCAAGAGGAGCAGTCCAGCGGCATGACCATTTTCTTCAGCCT
CCTTGTCCTAGCTATCTGCATCATATTGGTGCATTTACTGATCCGATACAGATTACATTT
CTTGCCAGAGAGTGTTGCTGTTGTTTCTTTAGGTATTCTCATGGGAGCAGTTATAAAAAT
TATAGAGTTTAAAAAACTGGCGAATTGGAAGGAAGAAGAAATGTTTCGTCCAAACATGTT
TTTCCTCCTCCTGCTTCCCCCTATTATCTTTGAGTCTGGATATTCATTACACAAGGGTAA
CTTCTTTCAAAATATTGGTTCCATCACCCTGTTTGCTGTTTTTGGGACGGCAATCTCCGC
TTTTGTAGTAGGTGGAGGAATTTATTTTCTGGGTCAGGCTGATGTAATCTCTAAACTCAA
CATGACAGACAGTTTTGCGTTTGGCTCCCTAATATCTGCTGTCGATCCAGTGGCCACTAT
TGCCATTTTCAATGCACTTCATGTGGACCCCGTGCTCAACATGCTGGTCTTTGGAGAAAG
TATTCTCAACGATGCAGTCTCCATTGTTCTGACCAACACAGCTGAAGGTTTAACAAGAAA
AAATATGTCAGATGTCAGTGGGTGGCAAACATTTTTACAAGCCCTTGACTACTTCCTCAA
AATGTTCTTTGGCTCTGCAGCGCTCGGCACTCTCACTGGCTTAATTTCTGCATTAGTGCT
GAAGCATATTGACTTGAGGAAAACGCCTTCCTTGGAGTTTGGCATGATGATCATTTTTGC
TTATCTGCCTTATGGGCTTGCAGAAGGAATCTCACTCTCAGGCATCATGGCCATCCTGTT
CTCAGGCATCGTGATGTCCCACTACACGCACCATAACCTCTCCCCAGTCACCCAGATCCT
CATGCAGCAGACCCTCCGCACCGTGGCCTTCTTATGTGAAACATGTGTGTTTGCATTTCT
TGGCCTGTCCATTTTTAGTTTTCCTCACAAGTTTGAAATTTCCTTTGTCATCTGGTGCAT
AGTGCTTGTACTATTTGGCAGAGCGGTAAACATTTTCCCTCTTTCCTACCTCCTGAATTT
CTTCCGGGATCATAAAATCACACCGAAGATGATGTTCATCATGTGGTTTAGTGGCCTGCG
GGGAGCCATCCCCTATGCCCTGAGCCTACACCTGGACCTGGAGCCCATGGAGAAGCGGCA
GCTCATCGGCACCACCACCATCGTCATCGTGCTCTTCACCATCCTGCTGCTGGGCGGCAG
CACCATGCCCCTCATTCGCCTCATGGACATCGAGGACGCCAAGGCACACCGCAGGAACAA
GAAGGACGTCAACCTCAGCAAGACTGAGAAGATGGGCAACACTGTGGAGTCGGAGCACCT
GTCGGAGCTCACGGAGGAGGAGTACGAGGCCCACTACATCAGGCGGCAGGACCTTAAGGG
CTTCGTGTGGCTGGACGCCAAGTACCTGAACCCCTTCTTCACTCGGAGGCTGACGCAGGA
GGACCTGCACCACGGGCGCATCCAGATGAAAACTCTCACCAACAAGTGGTACGAGGAGGT
ACGCCAGGGCCCCTCCGGCTCCGAGGACGACGAGCAGGAGCTGCTCTACGTAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT