KIAA0939

Product ID	ORK00690
Accession No	AB023156
Description	solute carrier family 9, subfamily A (NHE8, cation proton antiporter 8), member 8, transcript variant 2
Clone name	hh04825s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6087 bp) Predicted protein sequence (595 aa)
HaloTag ORF Clone	FHC00690
Flexi ORF Clone	FXC00690
Source	Human adult brain
Rouge ID	mKIAA0939 by Kazusa Mouse cDNA Project
Note	We replaced hh04825, former representative clones for KIAA0939 with hh04825s1. (2002/5/10)

Length: 6087 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	4299 bp
Genome contig ID	gi51511747f_47762825
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- TGGATGCCTATTTAGAAATAAAGTGTATGCTGCTG
Flanking genome sequence (179356 - 179405)	----+--------+--------+--------+--------+----* AATTGGAGCCAGTGTCTGTGTTCATGTTCAGGTGGCTTTGCAGATGCACA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	f	47862825	47942179	16	99.3	Perfect prediction

Length: 595 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_514716	99.5	Na+/H+ exchange...	Pan troglodytes
CAH18432	100.0	hypothetical pr...	Homo sapiens
EAW75648	99.3	solute carrier ...	Homo sapiens
Q9Y2E8	100.0	Sodium/hydrogen...	Homo sapiens
XP_854123	99.0	similar to Na+/...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D87743	4.8e-53	36.9	KIAA0267

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR004709	139	150	PR01084	Sodium/hydrogen exchanger subfamily
	IPR004709	153	167	PR01084	Sodium/hydrogen exchanger subfamily
	IPR004709	168	176	PR01084	Sodium/hydrogen exchanger subfamily
	IPR004709	208	218	PR01084	Sodium/hydrogen exchanger subfamily
HMMPfam	IPR006153	76	487	PF00999	Sodium/hydrogen exchanger
HMMTigr	IPR004709	67	590	TIGR00840	Sodium/hydrogen exchanger subfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	30	HEGFNVTLHTTLVVTTKLVLPTP	52	SECONDARY	23
	2	74	MTIFFSLLVLAICIILVHLLIR	95	PRIMARY	22
	3	102	PESVAVVSLGILMGAVIKIIEF	123	PRIMARY	22
	4	166	GSITLFAVFGTAISAFVVGGGIY	188	PRIMARY	23
	5	201	MTDSFAFGSLISAVDPVATIAIF	223	SECONDARY	23
	6	278	FLKMFFGSAALGTLTGLISALVL	300	SECONDARY	23
	7	328	EGISLSGIMAILFSGIVMSHYTH	350	PRIMARY	23
	8	366	RTVAFLCETCVFAFLGLSIFSFP	388	PRIMARY	23
	9	395	FVIWCIVLVLFGRAVNIFPLSYL	417	PRIMARY	23
	10	465	TTIVIVLFTILLLGGSTMPLIRL	487	SECONDARY	23

Primer_f	GTAAAAATGACGAGTGCTGGG
Primer_r	CCAAGAAGAGCAGCACATCAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-