(Circular) (Six-base) MAPSORT of: pFN21ASDA0939.seq Check: 3705 from: 1 to: 6472 pFN21ASDA0939 6472 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3489 4282 278 Size: 53 83 186 936 1953 793 2468 Fragments arranged by size: 2468 1953 936 793 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 2763 2900 4535 5919 6187 1632 Size: 565 566 137 1635 1384 268 1917 Fragments arranged by size: 1917 1635 1384 566 565 268 137 AfeI AGC'GCT Cuts at: 2841 2841 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 3593 820 Size: 197 2576 3699 Fragments arranged by size: 3699 2576 197 AflIII A'CryG_T Cuts at: 1203 2394 3101 5371 6348 1203 Size: 1191 707 2270 977 1327 Fragments arranged by size: 2270 1327 1191 977 707 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3440 1184 Size: 2256 4216 AlwNI CAG_nnn'CTG Cuts at: 3760 4215 5787 3760 Size: 455 1572 4445 Fragments arranged by size: 4445 1572 455 ApaI G_GGCC'C Cuts at: 3731 3731 Size: 6472 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 2538 3156 3235 3781 4007 4159 6268 1493 Size: 1045 618 79 546 226 152 2109 1697 Fragments arranged by size: 2109 1697 1045 618 546 226 152 79 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 3797 1870 Size: 73 1854 4545 Fragments arranged by size: 4545 1854 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3368 3793 5248 618 Size: 325 217 627 1581 425 1455 1842 Fragments arranged by size: 1842 1581 1455 627 425 325 217 BanII G_rGCy'C Cuts at: 1391 3345 3549 3731 3791 1391 Size: 1954 204 182 60 4072 Fragments arranged by size: 4072 1954 204 182 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5501 BbvCI CC'TCA_GC Cuts at: 3494 3494 Size: 6472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2104 2138 4663 4697 1903 Size: 34 167 34 2525 34 3678 Fragments arranged by size: 3678 2525 167 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 2928 1548 Size: 1380 5092 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2953 3735 5083 Size: 107 122 71 631 605 1281 782 1348 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 1525 1348 1281 782 631 605 122 107 71 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 3731 4533 5689 1790 Size: 1941 802 1156 2573 Fragments arranged by size: 2573 1941 1156 802 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3038 4203 5161 Size: 416 53 83 399 24 1621 1165 958 Cuts at: 5161 442 Size: 1753 Fragments arranged by size: 1753 1621 1165 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3359 5132 1239 Size: 2120 1773 2579 Fragments arranged by size: 2579 2120 1773 Bpu10I CC'TnA_GC Cuts at: 3320 3494 3320 Size: 174 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2914 4597 5462 5760 2914 Size: 1683 865 298 3626 Fragments arranged by size: 3626 1683 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5135 882 Size: 617 3636 2219 Fragments arranged by size: 3636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3769 4263 493 Size: 711 2565 494 2702 Fragments arranged by size: 2702 2565 711 494 BsaBI GATnn'nnATC Cuts at: 3275 6466 3275 Size: 3191 3281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3456 3486 3615 4279 Size: 53 83 186 936 1923 30 129 664 Cuts at: 4279 4661 5296 275 Size: 382 635 1451 Fragments arranged by size: 1923 1451 936 664 635 382 186 129 83 53 30 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2109 2139 3524 3545 3554 Size: 30 165 30 706 30 1385 21 9 Cuts at: 3554 3575 1178 Size: 21 4075 Fragments arranged by size: 4075 1385 706 165 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2198 2396 3569 3572 3728 1186 Size: 874 138 198 1173 3 156 3930 Fragments arranged by size: 3930 1173 874 198 156 138 3 BseYI C'CCAG_C Cuts at: 2175 3409 5675 6171 2175 Size: 1234 2266 496 2476 Fragments arranged by size: 2476 2266 1234 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 3650 1163 Size: 573 441 1473 3985 Fragments arranged by size: 3985 1473 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3922 4683 5711 1540 Size: 275 186 1921 761 1028 2301 Fragments arranged by size: 2301 1921 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2676 3393 3537 3549 4533 4618 5689 2127 Size: 549 717 144 12 984 85 1071 2910 Fragments arranged by size: 2910 1071 984 717 549 144 85 12 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2676 3345 3393 3537 3549 3731 Size: 399 337 549 669 48 144 12 182 Cuts at: 3731 3791 4533 4618 5689 1391 Size: 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 549 399 337 182 144 85 60 48 12 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 2068 4361 6091 2068 Size: 2293 1730 2449 Fragments arranged by size: 2449 2293 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3672 3827 844 Size: 2828 155 3489 Fragments arranged by size: 3489 2828 155 BsrBI CCG'CTC Cuts at: 1394 1823 3203 4359 1394 Size: 429 1380 1156 3507 Fragments arranged by size: 3507 1380 1156 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 5141 65 Size: 1183 3719 174 1396 Fragments arranged by size: 3719 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5116 5303 1700 Size: 110 3306 187 2869 Fragments arranged by size: 3306 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 4532 5544 4532 Size: 1012 5460 BstAPI GCAn_nnn'nTGC Cuts at: 794 3760 794 Size: 2966 3506 BstBI TT'CG_AA Cuts at: 2007 5276 2007 Size: 3269 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstYI r'GATC_y Cuts at: 1972 3054 3802 4169 4554 4571 6023 6109 6121 Size: 1082 748 367 385 17 1452 86 12 Cuts at: 6121 6467 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1082 748 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3081 3345 3670 513 Size: 552 957 1059 264 325 3315 Fragments arranged by size: 3315 1059 957 552 325 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5733 Fragments arranged by size: 5733 683 56 BtsI GCAGTG_nn' Cuts at: 3988 4782 4802 3988 Size: 794 20 5658 Fragments arranged by size: 5658 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2086 2121 529 Size: 35 1522 35 4880 Fragments arranged by size: 4880 1522 35 35 DraI TTT'AAA Cuts at: 2350 3776 2350 Size: 1426 5046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2631 2988 3919 Size: 54 1425 50 275 18 801 357 931 Cuts at: 3919 4808 6437 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 931 889 801 357 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 3398 4402 1353 Size: 684 1361 1004 3423 Fragments arranged by size: 3423 1361 1004 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 5059 5433 5579 387 Size: 1064 138 465 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 3547 3547 Size: 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2763 2900 3359 4535 5132 5919 Size: 393 565 566 137 459 1176 597 787 Cuts at: 5919 6187 1239 Size: 268 1524 Fragments arranged by size: 1524 1176 787 597 566 565 459 393 268 137 EcoO109I rG'GnC_Cy Cuts at: 3589 3661 3727 3728 3589 Size: 72 66 1 6333 Fragments arranged by size: 6333 72 66 1 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2843 5619 1678 Size: 1165 2776 2531 Fragments arranged by size: 2776 2531 1165 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 2552 2584 2702 Size: 32 135 32 602 32 346 32 118 Cuts at: 2702 2734 3524 3556 3676 3708 5113 5145 5187 Size: 32 790 32 120 32 1405 32 42 Cuts at: 5187 5219 1373 Size: 32 2626 Fragments arranged by size: 2626 1405 790 602 346 135 120 118 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3490 3816 4062 669 Size: 988 281 1552 326 246 3079 Fragments arranged by size: 3079 1552 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3510 3522 5586 5770 1305 Size: 2205 12 2064 184 2007 Fragments arranged by size: 2205 2064 2007 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2633 2990 10 Size: 54 1425 343 801 357 3492 Fragments arranged by size: 3492 1425 801 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 3011 3683 4430 4789 4948 518 Size: 648 1080 765 672 747 359 159 2042 Fragments arranged by size: 2042 1080 765 747 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2196 2741 3888 4565 5713 5958 1606 Size: 590 545 1147 677 1148 245 2120 Fragments arranged by size: 2120 1148 1147 677 590 545 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3345 513 Size: 552 957 1323 3640 Fragments arranged by size: 3640 1323 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 2398 2683 3105 3830 5375 1132 Size: 97 1169 285 422 725 1545 2229 Fragments arranged by size: 2229 1545 1169 725 422 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 2394 3101 5371 2394 Size: 707 2270 3495 Fragments arranged by size: 3495 2270 707 PflMI CCAn_nnn'nTGG Cuts at: 1108 3705 1108 Size: 2597 3875 PfoI T'CCnGG_A Cuts at: 3243 3243 Size: 6472 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2036 2068 4541 4573 6054 6086 2036 Size: 32 2473 32 1481 32 2422 Fragments arranged by size: 2473 2422 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3589 3661 3589 Size: 72 6400 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 2333 4042 2333 Size: 1709 4763 PspOMI G'GGCC_C Cuts at: 3727 3727 Size: 6472 PstI C_TGCA'G Cuts at: 830 2839 3824 830 Size: 2009 985 3478 Fragments arranged by size: 3478 2009 985 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2741 2741 Size: 6472 SacI G_AGCT'C Cuts at: 3549 3549 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 3398 3398 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2835 3820 4955 5636 5827 826 Size: 219 1790 985 1135 681 191 1471 Fragments arranged by size: 1790 1471 1135 985 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3799 1872 Size: 1927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2893 3593 4576 5477 5739 820 Size: 197 926 950 700 983 901 262 1553 Fragments arranged by size: 1553 983 950 926 901 700 262 197 SnaBI TAC'GTA Cuts at: 493 3769 4263 493 Size: 3276 494 2702 Fragments arranged by size: 3276 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 2473 4396 5 Size: 47 2421 1923 2081 Fragments arranged by size: 2421 2081 1923 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2022 2910 3345 3459 3911 513 Size: 552 957 888 435 114 452 3074 Fragments arranged by size: 3074 957 888 552 452 435 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2769 3065 4499 4516 4669 4854 5299 Size: 1255 1189 296 1434 17 153 185 445 Cuts at: 5299 325 Size: 1498 Fragments arranged by size: 1498 1434 1255 1189 445 296 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3187 4718 6430 Size: 274 80 33 51 1443 1210 1531 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 1712 1531 1443 1210 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6432 6464 6432 Size: 32 6440 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3406 3406 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3797 1870 Size: 1927 4545 XmnI GAAnn'nnTTC Cuts at: 2382 2700 3239 3632 4601 2382 Size: 318 539 393 969 4253 Fragments arranged by size: 4253 969 539 393 318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3487 4280 276 Size: 53 83 186 936 1953 793 2468 Fragments arranged by size: 2468 1953 936 793 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI