PLXNB2 ORF clone FHC00501

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00501

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Clone info.

Species

human

Clone Name

pFN21ASDA0315 Sequence (with vector) Restriction Map

Product ID

FHC00501

Flexi Clone PID

FXC00501

ORF Sequence

DNA sequence (5514 bp)
Protein sequence (1838 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383845]

Gene

KIAA0315

Symbol

PLXNB2

Alias : MM1, Nbla00445, PLEXB2, dJ402G11.3

Description

plexin B2

Original Clone ID

hg00246

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5514 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1838 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43035.1 0 100.0 PLXNB2 CCDS33854.1 7.3e-109 34.7 PLXND1 CCDS2765.1 1e-90 39.3 PLXNB1 CCDS33847.2 4.9e-68 33.8 PLXNA1 CCDS14752.1 2.7e-65 33.0 PLXNA3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001627 35 435 PF01403 Semaphorin/CD100 antigen IPR002165 469 516 PF01437 Plexin IPR002909 803 892 PF01833 Cell surface receptor IPT/TIG IPR002909 896 972 PF01833 Cell surface receptor IPT/TIG IPR002909 983 1091 PF01833 Cell surface receptor IPT/TIG IPR013548 1271 1805 PF08337 Plexin HMMSmart IPR001627 34 450 SM00630 Semaphorin/CD100 antigen IPR003659 468 519 SM00423 Plexin/semaphorin/integrin IPR003659 614 667 SM00423 Plexin/semaphorin/integrin IPR003659 759 801 SM00423 Plexin/semaphorin/integrin IPR002909 802 893 SM00429 Cell surface receptor IPT/TIG IPR002909 894 980 SM00429 Cell surface receptor IPT/TIG IPR002909 982 1092 SM00429 Cell surface receptor IPT/TIG ProfileScan IPR001627 8 466 PS51004 Semaphorin/CD100 antigen
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MALQLWALTLLGLLGAGASLR 21 PRIMARY 21 2 35 NHLAVDEASGVVYLGAVNALYQL 57 SECONDARY 23 3 1196 VPLSLILPLVIVPMVVVIAVSV 1217 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016884192 1838 604293 100.0 100.0 NP_036533 1838 604293 100.0 100.0 XP_005261968 1838 604293 100.0 100.0 XP_005261966 1838 604293 100.0 100.0 XP_006724476 1838 604293 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0315 Download

>KIAA0315  5514 bp
ATGGCACTGCAGCTCTGGGCCCTGACCCTGCTGGGCCTGCTGGGCGCAGGTGCCAGCCTG
AGGCCCCGCAAGCTGGACTTCTTCCGCAGCGAGAAAGAGCTGAACCACCTGGCTGTGGAT
GAGGCCTCAGGCGTGGTGTACCTGGGGGCGGTGAATGCCCTCTACCAGCTGGATGCGAAG
CTGCAGCTGGAGCAGCAGGTGGCCACGGGCCCGGCCCTGGACAACAAGAAGTGCACGCCG
CCCATCGAGGCCAGCCAGTGCCATGAGGCTGAGATGACTGACAATGTCAACCAGCTGCTG
CTGCTCGACCCTCCCAGGAAGCGCCTGGTGGAGTGCGGCAGCCTCTTCAAGGGCATCTGC
GCTCTGCGCGCCCTGAGCAACATCTCCCTCCGCCTGTTCTACGAGGACGGCAGCGGGGAG
AAGTCTTTCGTGGCCAGCAATGATGAGGGCGTGGCCACAGTGGGGCTGGTGAGCTCCACG
GGTCCTGGTGGTGACCGCGTGCTGTTTGTGGGCAAAGGCAATGGGCCACACGACAACGGC
ATCATCGTGAGCACTCGGCTGTTGGACCGGACTGACAGCAGGGAGGCCTTTGAAGCCTAC
ACGGACCACGCCACCTACAAGGCCGGCTACCTGTCCACCAACACACAGCAGTTCGTGGCG
GCCTTCGAGGACGGCCCCTACGTCTTCTTTGTCTTCAACCAGCAGGACAAGCACCCGGCC
CGGAACCGCACGCTGCTGGCACGCATGTGCAGAGAAGACCCCAACTACTACTCCTACCTG
GAGATGGACCTGCAGTGCCGGGACCCCGACATCCACGCCGCTGCCTTTGGCACCTGCCTG
GCCGCCTCCGTGGCTGCGCCTGGCTCTGGCAGGGTGCTATATGCTGTCTTCAGCAGAGAC
AGCCGGAGCAGTGGGGGGCCCGGTGCGGGCCTCTGCCTGTTCCCGCTGGACAAGGTGCAC
GCCAAGATGGAGGCCAACCGCAACGCCTGTTACACAGGCACCCGGGAGGCCCGTGACATC
TTCTACAAGCCCTTCCACGGCGATATCCAGTGCGGCGGCCACGCGCCGGGCTCCAGCAAG
AGCTTCCCATGTGGCTCGGAGCACCTGCCCTACCCGCTGGGCAGCCGCGACGGGCTCAGA
GGCACAGCCGTGCTGCAGCGTGGAGGCCTGAACCTCACGGCCGTGACGGTCGCCGCCGAG
AACAACCACACTGTTGCTTTTCTGGGCACCTCTGATGGCCGGATCCTCAAGGTGTACCTC
ACCCCAGATGGCACCTCCTCAGAGTACGACTCTATCCTTGTGGAGATAAACAAGAGAGTC
AAGCGCGACCTGGTACTGTCTGGAGACCTGGGCAGCCTGTACGCCATGACCCAGGACAAG
GTGTTCCGGCTGCCGGTGCAGGAGTGCCTGAGCTACCCGACCTGCACCCAGTGCCGCGAC
TCCCAGGACCCCTACTGCGGCTGGTGCGTCGTCGAGGGACGATGCACCCGGAAGGCCGAG
TGTCCGCGGGCCGAGGAGGCCAGCCACTGGCTGTGGAGCCGAAGCAAGTCCTGCGTGGCC
GTCACCAGCGCCCAGCCACAGAACATGAGCCGGCGGGCCCAGGGGGAGGTGCAGCTGACC
GTCAGCCCCCTCCCTGCCCTGAGCGAGGAGGACGAGTTGCTGTGCCTTTTTGGGGAGTCG
CCGCCACACCCCGCCCGCGTGGAGGGCGAGGCCGTCATCTGCAACTCCCCAAGCAGCATC
CCCGTCACACCGCCAGGCCAGGACCACGTGGCCGTGACCATCCAGCTCCTCCTTAGACGA
GGCAACATCTTCCTCACGTCCTACCAGTACCCCTTCTACGACTGCCGCCAGGCCATGAGC
CTGGAGGAGAACCTGCCGTGCATCTCCTGCGTGAGCAACCGCTGGACCTGCCAGTGGGAC
CTGCGCTACCACGAGTGCCGGGAGGCTTCGCCCAACCCTGAGGACGGCATCGTCCGTGCC
CACATGGAGGACAGCTGTCCCCAGTTCCTGGGACCCAGCCCCCTGGTGATCCCCATGAAC
CACGAGACAGATGTGAACTTCCAGGGCAAGAACCTGGACACCGTGAAGGGTTCCTCCCTG
CACGTGGGCAGTGACTTGCTCAAGTTCATGGAGCCGGTGACCATGCAGGAATCTGGGACC
TTCGCCTTTCGGACCCCAAAGCTGTCCCACGATGCCAACGAGACGCTGCCCCTGCACCTC
TACGTCAAGTCTTACGGCAAGAATATCGACAGCAAGCTCCATGTGACCCTCTACAACTGC
TCCTTTGGCCGCAGCGACTGCAGCCTGTGCCGGGCCGCTAACCCCGACTACAGGTGTGCG
TGGTGCGGGGGCCAGAGCAGGTGCGTGTATGAGGCCCTGTGCAACACCACCTCCGAGTGC
CCGCCGCCCGTCATCACCAGGATCCAGCCTGAGACGGGCCCCCTGGGTGGGGGCATCCGC
ATCACCATCCTGGGGTCCAATTTGGGCGTCCAAGCAGGGGACATCCAGAGGATCTCTGTG
GCCGGCCGGAACTGCTCCTTTCAGCCGGAACGTTACTCCGTGTCCACCCGGATCGTGTGT
GTGATCGAGGCTGCGGAGACGCCTTTCACGGGGGGTGTCGAGGTGGACGTCTTCGGGAAA
CTGGGCCGTTCGCCTCCCAATGTCCAGTTCACCTTCCAACAGCCCAAGCCTCTCAGTGTG
GAGCCGCAGCAGGGACCGCAGGCGGGCGGCACCACACTGACCATCCACGGCACCCACCTG
GACACGGGCTCCCAGGAGGACGTGCGGGTGACCCTCAACGGCGTCCCGTGTAAAGTGACG
AAGTTTGGGGCGCAGCTCCAGTGTGTCACTGGCCCCCAGGCGACACGGGGCCAGATGCTT
CTGGAGGTCTCCTACGGGGGGTCCCCCGTGCCCAACCCCGGCATCTTCTTCACCTACCGC
GAAAACCCCGTACTGCGAGCCTTCGAGCCGCTACGAAGCTTTGCCAGTGGTGGCCGCAGC
ATCAACGTCACGGGTCAGGGCTTCAGCCTGATCCAGAGGTTTGCCATGGTGGTCATCGCG
GAGCCCCTGCAGTCCTGGCAGCCGCCGCGGGAGGCTGAATCCCTGCAGCCCATGACGGTG
GTGGGTACAGACTACGTGTTCCACAATGACACCAAGGTCGTCTTCCTGTCCCCGGCTGTG
CCTGAGGAGCCAGAGGCCTACAACCTCACGGTGCTGATCGAGATGGACGGGCACCGTGCC
CTGCTCAGAACAGAGGCCGGGGCCTTCGAGTACGTGCCTGACCCCACCTTTGAGAACTTC
ACAGGTGGCGTCAAGAAGCAGGTCAACAAGCTCATCCACGCCCGGGGCACCAATCTGAAC
AAGGCGATGACGCTGCAGGAGGCCGAGGCCTTCGTGGGTGCCGAGCGCTGCACCATGAAG
ACGCTGACGGAGACCGACCTGTACTGTGAGCCCCCGGAGGTGCAGCCCCCGCCCAAGCGG
CGGCAGAAACGAGACACCACACACAACCTGCCCGAGTTCATTGTGAAGTTCGGCTCTCGC
GAGTGGGTGCTGGGCCGCGTGGAGTACGACACACGGGTGAGCGACGTGCCGCTCAGCCTC
ATCTTGCCGCTGGTCATCGTGCCCATGGTGGTCGTCATCGCGGTGTCTGTCTACTGCTAC
TGGAGGAAGAGCCAGCAGGCCGAACGAGAGTATGAGAAGATCAAGTCCCAGCTGGAGGGC
CTGGAGGAGAGCGTGCGGGACCGCTGCAAGAAGGAATTCACAGACCTGATGATCGAGATG
GAGGACCAGACCAACGACGTGCACGAGGCCGGCATCCCCGTGCTGGACTACAAGACCTAC
ACCGACCGCGTCTTCTTCCTGCCCTCCAAGGACGGCGACAAGGACGTGATGATCACCGGC
AAGCTGGACATCCCTGAGCCGCGGCGGCCGGTGGTGGAGCAGGCCCTCTACCAGTTCTCC
AACCTGCTGAACAGCAAGTCTTTCCTCATCAATTTCATCCACACCCTGGAGAACCAGCGG
GAGTTCTCGGCCCGCGCCAAGGTCTACTTCGCGTCCCTGCTGACGGTGGCGCTGCACGGG
AAACTGGAGTACTACACGGACATCATGCACACGCTCTTCCTGGAGCTCCTGGAGCAGTAC
GTGGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGTCTGAGACTGTGGTGGAGAGGATG
CTGTCCAACTGGATGTCCATCTGCCTGTACCAGTACCTCAAGGACAGTGCCGGGGAGCCC
CTGTACAAGCTCTTCAAGGCCATCAAACATCAGGTGGAAAAGGGCCCGGTGGATGCGGTA
CAGAAGAAGGCCAAGTACACTCTCAACGACACGGGGCTGCTGGGGGATGATGTGGAGTAC
GCACCCCTGACGGTGAGCGTGATCGTGCAGGACGAGGGAGTGGACGCCATCCCGGTGAAG
GTCCTCAACTGTGACACCATCTCCCAGGTCAAGGAGAAGATCATTGACCAGGTGTACCGT
GGGCAGCCCTGCTCCTGCTGGCCCAGGCCAGACAGCGTGGTCCTGGAGTGGCGTCCGGGC
TCCACAGCGCAGATCCTGTCGGACCTGGACCTGACGTCACAGCGGGAGGGCCGGTGGAAG
CGCGTCAACACCCTTATGCACTACAATGTCCGGGATGGAGCCACCCTCATCCTGTCCAAG
GTGGGGGTCTCCCAGCAGCCGGAGGACAGCCAGCAGGACCTGCCTGGGGAGCGCCATGCC
CTCCTGGAGGAGGAGAACCGGGTGTGGCACCTGGTGCGGCCGACCGACGAGGTGGACGAG
GGCAAGTCCAAGAGAGGCAGCGTGAAAGAGAAGGAGCGGACGAAGGCCATCACCGAGATC
TACCTGACGCGGCTGCTCTCAGTCAAGGGCACACTGCAGCAGTTTGTGGACAACTTCTTC
CAGAGCGTGCTGGCGCCTGGGCACGCGGTGCCACCTGCAGTCAAGTACTTCTTCGACTTC
CTGGACGAGCAGGCAGAGAAGCACAACATCCAGGATGAAGACACCATCCACATCTGGAAG
ACGAACAGCTTACCGCTCCGGTTCTGGGTGAACATCCTCAAGAACCCCCACTTCATCTTT
GACGTGCATGTCCACGAGGTGGTGGACGCCTCGCTGTCAGTCATCGCGCAGACCTTCATG
GATGCCTGCACGCGCACGGAGCATAAGCTGAGCCGCGATTCTCCCAGCAACAAGCTGCTG
TACGCCAAGGAGATCTCCACCTACAAGAAGATGGTGGAGGATTACTACAAGGGGATCCGG
CAGATGGTGCAGGTCAGCGACCAGGACATGAACACACACCTGGCAGAGATTTCCCGGGCG
CACACGGACTCCTTGAACACCCTCGTGGCACTCCACCAGCTCTACCAATACACGCAGAAG
TACTATGACGAGATCATCAATGCCTTGGAGGAGGATCCTGCCGCCCAGAAGATGCAGCTG
GCCTTCCGCCTGCAGCAGATTGCCGCTGCACTGGAGAACAAGGTCACTGACCTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0315 Download

>KIAA0315  1838 aa
MALQLWALTLLGLLGAGASLRPRKLDFFRSEKELNHLAVDEASGVVYLGAVNALYQLDAK
LQLEQQVATGPALDNKKCTPPIEASQCHEAEMTDNVNQLLLLDPPRKRLVECGSLFKGIC
ALRALSNISLRLFYEDGSGEKSFVASNDEGVATVGLVSSTGPGGDRVLFVGKGNGPHDNG
IIVSTRLLDRTDSREAFEAYTDHATYKAGYLSTNTQQFVAAFEDGPYVFFVFNQQDKHPA
RNRTLLARMCREDPNYYSYLEMDLQCRDPDIHAAAFGTCLAASVAAPGSGRVLYAVFSRD
SRSSGGPGAGLCLFPLDKVHAKMEANRNACYTGTREARDIFYKPFHGDIQCGGHAPGSSK
SFPCGSEHLPYPLGSRDGLRGTAVLQRGGLNLTAVTVAAENNHTVAFLGTSDGRILKVYL
TPDGTSSEYDSILVEINKRVKRDLVLSGDLGSLYAMTQDKVFRLPVQECLSYPTCTQCRD
SQDPYCGWCVVEGRCTRKAECPRAEEASHWLWSRSKSCVAVTSAQPQNMSRRAQGEVQLT
VSPLPALSEEDELLCLFGESPPHPARVEGEAVICNSPSSIPVTPPGQDHVAVTIQLLLRR
GNIFLTSYQYPFYDCRQAMSLEENLPCISCVSNRWTCQWDLRYHECREASPNPEDGIVRA
HMEDSCPQFLGPSPLVIPMNHETDVNFQGKNLDTVKGSSLHVGSDLLKFMEPVTMQESGT
FAFRTPKLSHDANETLPLHLYVKSYGKNIDSKLHVTLYNCSFGRSDCSLCRAANPDYRCA
WCGGQSRCVYEALCNTTSECPPPVITRIQPETGPLGGGIRITILGSNLGVQAGDIQRISV
AGRNCSFQPERYSVSTRIVCVIEAAETPFTGGVEVDVFGKLGRSPPNVQFTFQQPKPLSV
EPQQGPQAGGTTLTIHGTHLDTGSQEDVRVTLNGVPCKVTKFGAQLQCVTGPQATRGQML
LEVSYGGSPVPNPGIFFTYRENPVLRAFEPLRSFASGGRSINVTGQGFSLIQRFAMVVIA
EPLQSWQPPREAESLQPMTVVGTDYVFHNDTKVVFLSPAVPEEPEAYNLTVLIEMDGHRA
LLRTEAGAFEYVPDPTFENFTGGVKKQVNKLIHARGTNLNKAMTLQEAEAFVGAERCTMK
TLTETDLYCEPPEVQPPPKRRQKRDTTHNLPEFIVKFGSREWVLGRVEYDTRVSDVPLSL
ILPLVIVPMVVVIAVSVYCYWRKSQQAEREYEKIKSQLEGLEESVRDRCKKEFTDLMIEM
EDQTNDVHEAGIPVLDYKTYTDRVFFLPSKDGDKDVMITGKLDIPEPRRPVVEQALYQFS
NLLNSKSFLINFIHTLENQREFSARAKVYFASLLTVALHGKLEYYTDIMHTLFLELLEQY
VVAKNPKLMLRRSETVVERMLSNWMSICLYQYLKDSAGEPLYKLFKAIKHQVEKGPVDAV
QKKAKYTLNDTGLLGDDVEYAPLTVSVIVQDEGVDAIPVKVLNCDTISQVKEKIIDQVYR
GQPCSCWPRPDSVVLEWRPGSTAQILSDLDLTSQREGRWKRVNTLMHYNVRDGATLILSK
VGVSQQPEDSQQDLPGERHALLEEENRVWHLVRPTDEVDEGKSKRGSVKEKERTKAITEI
YLTRLLSVKGTLQQFVDNFFQSVLAPGHAVPPAVKYFFDFLDEQAEKHNIQDEDTIHIWK
TNSLPLRFWVNILKNPHFIFDVHVHEVVDASLSVIAQTFMDACTRTEHKLSRDSPSNKLL
YAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTDSLNTLVALHQLYQYTQK
YYDEIINALEEDPAAQKMQLAFRLQQIAAALENKVTDL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0315 Download

>pFN21ASDA0315  10243 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCACTGCAGCTCTG
GGCCCTGACCCTGCTGGGCCTGCTGGGCGCAGGTGCCAGCCTGAGGCCCCGCAAGCTGGA
CTTCTTCCGCAGCGAGAAAGAGCTGAACCACCTGGCTGTGGATGAGGCCTCAGGCGTGGT
GTACCTGGGGGCGGTGAATGCCCTCTACCAGCTGGATGCGAAGCTGCAGCTGGAGCAGCA
GGTGGCCACGGGCCCGGCCCTGGACAACAAGAAGTGCACGCCGCCCATCGAGGCCAGCCA
GTGCCATGAGGCTGAGATGACTGACAATGTCAACCAGCTGCTGCTGCTCGACCCTCCCAG
GAAGCGCCTGGTGGAGTGCGGCAGCCTCTTCAAGGGCATCTGCGCTCTGCGCGCCCTGAG
CAACATCTCCCTCCGCCTGTTCTACGAGGACGGCAGCGGGGAGAAGTCTTTCGTGGCCAG
CAATGATGAGGGCGTGGCCACAGTGGGGCTGGTGAGCTCCACGGGTCCTGGTGGTGACCG
CGTGCTGTTTGTGGGCAAAGGCAATGGGCCACACGACAACGGCATCATCGTGAGCACTCG
GCTGTTGGACCGGACTGACAGCAGGGAGGCCTTTGAAGCCTACACGGACCACGCCACCTA
CAAGGCCGGCTACCTGTCCACCAACACACAGCAGTTCGTGGCGGCCTTCGAGGACGGCCC
CTACGTCTTCTTTGTCTTCAACCAGCAGGACAAGCACCCGGCCCGGAACCGCACGCTGCT
GGCACGCATGTGCAGAGAAGACCCCAACTACTACTCCTACCTGGAGATGGACCTGCAGTG
CCGGGACCCCGACATCCACGCCGCTGCCTTTGGCACCTGCCTGGCCGCCTCCGTGGCTGC
GCCTGGCTCTGGCAGGGTGCTATATGCTGTCTTCAGCAGAGACAGCCGGAGCAGTGGGGG
GCCCGGTGCGGGCCTCTGCCTGTTCCCGCTGGACAAGGTGCACGCCAAGATGGAGGCCAA
CCGCAACGCCTGTTACACAGGCACCCGGGAGGCCCGTGACATCTTCTACAAGCCCTTCCA
CGGCGATATCCAGTGCGGCGGCCACGCGCCGGGCTCCAGCAAGAGCTTCCCATGTGGCTC
GGAGCACCTGCCCTACCCGCTGGGCAGCCGCGACGGGCTCAGAGGCACAGCCGTGCTGCA
GCGTGGAGGCCTGAACCTCACGGCCGTGACGGTCGCCGCCGAGAACAACCACACTGTTGC
TTTTCTGGGCACCTCTGATGGCCGGATCCTCAAGGTGTACCTCACCCCAGATGGCACCTC
CTCAGAGTACGACTCTATCCTTGTGGAGATAAACAAGAGAGTCAAGCGCGACCTGGTACT
GTCTGGAGACCTGGGCAGCCTGTACGCCATGACCCAGGACAAGGTGTTCCGGCTGCCGGT
GCAGGAGTGCCTGAGCTACCCGACCTGCACCCAGTGCCGCGACTCCCAGGACCCCTACTG
CGGCTGGTGCGTCGTCGAGGGACGATGCACCCGGAAGGCCGAGTGTCCGCGGGCCGAGGA
GGCCAGCCACTGGCTGTGGAGCCGAAGCAAGTCCTGCGTGGCCGTCACCAGCGCCCAGCC
ACAGAACATGAGCCGGCGGGCCCAGGGGGAGGTGCAGCTGACCGTCAGCCCCCTCCCTGC
CCTGAGCGAGGAGGACGAGTTGCTGTGCCTTTTTGGGGAGTCGCCGCCACACCCCGCCCG
CGTGGAGGGCGAGGCCGTCATCTGCAACTCCCCAAGCAGCATCCCCGTCACACCGCCAGG
CCAGGACCACGTGGCCGTGACCATCCAGCTCCTCCTTAGACGAGGCAACATCTTCCTCAC
GTCCTACCAGTACCCCTTCTACGACTGCCGCCAGGCCATGAGCCTGGAGGAGAACCTGCC
GTGCATCTCCTGCGTGAGCAACCGCTGGACCTGCCAGTGGGACCTGCGCTACCACGAGTG
CCGGGAGGCTTCGCCCAACCCTGAGGACGGCATCGTCCGTGCCCACATGGAGGACAGCTG
TCCCCAGTTCCTGGGACCCAGCCCCCTGGTGATCCCCATGAACCACGAGACAGATGTGAA
CTTCCAGGGCAAGAACCTGGACACCGTGAAGGGTTCCTCCCTGCACGTGGGCAGTGACTT
GCTCAAGTTCATGGAGCCGGTGACCATGCAGGAATCTGGGACCTTCGCCTTTCGGACCCC
AAAGCTGTCCCACGATGCCAACGAGACGCTGCCCCTGCACCTCTACGTCAAGTCTTACGG
CAAGAATATCGACAGCAAGCTCCATGTGACCCTCTACAACTGCTCCTTTGGCCGCAGCGA
CTGCAGCCTGTGCCGGGCCGCTAACCCCGACTACAGGTGTGCGTGGTGCGGGGGCCAGAG
CAGGTGCGTGTATGAGGCCCTGTGCAACACCACCTCCGAGTGCCCGCCGCCCGTCATCAC
CAGGATCCAGCCTGAGACGGGCCCCCTGGGTGGGGGCATCCGCATCACCATCCTGGGGTC
CAATTTGGGCGTCCAAGCAGGGGACATCCAGAGGATCTCTGTGGCCGGCCGGAACTGCTC
CTTTCAGCCGGAACGTTACTCCGTGTCCACCCGGATCGTGTGTGTGATCGAGGCTGCGGA
GACGCCTTTCACGGGGGGTGTCGAGGTGGACGTCTTCGGGAAACTGGGCCGTTCGCCTCC
CAATGTCCAGTTCACCTTCCAACAGCCCAAGCCTCTCAGTGTGGAGCCGCAGCAGGGACC
GCAGGCGGGCGGCACCACACTGACCATCCACGGCACCCACCTGGACACGGGCTCCCAGGA
GGACGTGCGGGTGACCCTCAACGGCGTCCCGTGTAAAGTGACGAAGTTTGGGGCGCAGCT
CCAGTGTGTCACTGGCCCCCAGGCGACACGGGGCCAGATGCTTCTGGAGGTCTCCTACGG
GGGGTCCCCCGTGCCCAACCCCGGCATCTTCTTCACCTACCGCGAAAACCCCGTACTGCG
AGCCTTCGAGCCGCTACGAAGCTTTGCCAGTGGTGGCCGCAGCATCAACGTCACGGGTCA
GGGCTTCAGCCTGATCCAGAGGTTTGCCATGGTGGTCATCGCGGAGCCCCTGCAGTCCTG
GCAGCCGCCGCGGGAGGCTGAATCCCTGCAGCCCATGACGGTGGTGGGTACAGACTACGT
GTTCCACAATGACACCAAGGTCGTCTTCCTGTCCCCGGCTGTGCCTGAGGAGCCAGAGGC
CTACAACCTCACGGTGCTGATCGAGATGGACGGGCACCGTGCCCTGCTCAGAACAGAGGC
CGGGGCCTTCGAGTACGTGCCTGACCCCACCTTTGAGAACTTCACAGGTGGCGTCAAGAA
GCAGGTCAACAAGCTCATCCACGCCCGGGGCACCAATCTGAACAAGGCGATGACGCTGCA
GGAGGCCGAGGCCTTCGTGGGTGCCGAGCGCTGCACCATGAAGACGCTGACGGAGACCGA
CCTGTACTGTGAGCCCCCGGAGGTGCAGCCCCCGCCCAAGCGGCGGCAGAAACGAGACAC
CACACACAACCTGCCCGAGTTCATTGTGAAGTTCGGCTCTCGCGAGTGGGTGCTGGGCCG
CGTGGAGTACGACACACGGGTGAGCGACGTGCCGCTCAGCCTCATCTTGCCGCTGGTCAT
CGTGCCCATGGTGGTCGTCATCGCGGTGTCTGTCTACTGCTACTGGAGGAAGAGCCAGCA
GGCCGAACGAGAGTATGAGAAGATCAAGTCCCAGCTGGAGGGCCTGGAGGAGAGCGTGCG
GGACCGCTGCAAGAAGGAATTCACAGACCTGATGATCGAGATGGAGGACCAGACCAACGA
CGTGCACGAGGCCGGCATCCCCGTGCTGGACTACAAGACCTACACCGACCGCGTCTTCTT
CCTGCCCTCCAAGGACGGCGACAAGGACGTGATGATCACCGGCAAGCTGGACATCCCTGA
GCCGCGGCGGCCGGTGGTGGAGCAGGCCCTCTACCAGTTCTCCAACCTGCTGAACAGCAA
GTCTTTCCTCATCAATTTCATCCACACCCTGGAGAACCAGCGGGAGTTCTCGGCCCGCGC
CAAGGTCTACTTCGCGTCCCTGCTGACGGTGGCGCTGCACGGGAAACTGGAGTACTACAC
GGACATCATGCACACGCTCTTCCTGGAGCTCCTGGAGCAGTACGTGGTGGCCAAGAACCC
CAAGCTGATGCTGCGCAGGTCTGAGACTGTGGTGGAGAGGATGCTGTCCAACTGGATGTC
CATCTGCCTGTACCAGTACCTCAAGGACAGTGCCGGGGAGCCCCTGTACAAGCTCTTCAA
GGCCATCAAACATCAGGTGGAAAAGGGCCCGGTGGATGCGGTACAGAAGAAGGCCAAGTA
CACTCTCAACGACACGGGGCTGCTGGGGGATGATGTGGAGTACGCACCCCTGACGGTGAG
CGTGATCGTGCAGGACGAGGGAGTGGACGCCATCCCGGTGAAGGTCCTCAACTGTGACAC
CATCTCCCAGGTCAAGGAGAAGATCATTGACCAGGTGTACCGTGGGCAGCCCTGCTCCTG
CTGGCCCAGGCCAGACAGCGTGGTCCTGGAGTGGCGTCCGGGCTCCACAGCGCAGATCCT
GTCGGACCTGGACCTGACGTCACAGCGGGAGGGCCGGTGGAAGCGCGTCAACACCCTTAT
GCACTACAATGTCCGGGATGGAGCCACCCTCATCCTGTCCAAGGTGGGGGTCTCCCAGCA
GCCGGAGGACAGCCAGCAGGACCTGCCTGGGGAGCGCCATGCCCTCCTGGAGGAGGAGAA
CCGGGTGTGGCACCTGGTGCGGCCGACCGACGAGGTGGACGAGGGCAAGTCCAAGAGAGG
CAGCGTGAAAGAGAAGGAGCGGACGAAGGCCATCACCGAGATCTACCTGACGCGGCTGCT
CTCAGTCAAGGGCACACTGCAGCAGTTTGTGGACAACTTCTTCCAGAGCGTGCTGGCGCC
TGGGCACGCGGTGCCACCTGCAGTCAAGTACTTCTTCGACTTCCTGGACGAGCAGGCAGA
GAAGCACAACATCCAGGATGAAGACACCATCCACATCTGGAAGACGAACAGCTTACCGCT
CCGGTTCTGGGTGAACATCCTCAAGAACCCCCACTTCATCTTTGACGTGCATGTCCACGA
GGTGGTGGACGCCTCGCTGTCAGTCATCGCGCAGACCTTCATGGATGCCTGCACGCGCAC
GGAGCATAAGCTGAGCCGCGATTCTCCCAGCAACAAGCTGCTGTACGCCAAGGAGATCTC
CACCTACAAGAAGATGGTGGAGGATTACTACAAGGGGATCCGGCAGATGGTGCAGGTCAG
CGACCAGGACATGAACACACACCTGGCAGAGATTTCCCGGGCGCACACGGACTCCTTGAA
CACCCTCGTGGCACTCCACCAGCTCTACCAATACACGCAGAAGTACTATGACGAGATCAT
CAATGCCTTGGAGGAGGATCCTGCCGCCCAGAAGATGCAGCTGGCCTTCCGCCTGCAGCA
GATTGCCGCTGCACTGGAGAACAAGGTCACTGACCTCTACGTAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT