Restriction map for
pFN21ASDA0315
(Linear) (Six-base) MAPSORT of: KIAA0315.seq Check: 2231 from: 1 to: 5514
KIAA0315.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 37 186 841 1112 2348 4962 5514
Size: 37 149 655 271 1236 2614 552
Fragments arranged by size:
2614 1236 655 552 271 149 37
AatII G_ACGT'C
Cuts at: 0 2630 4597 5514
Size: 2630 1967 917
Fragments arranged by size:
2630 1967 917
AccI GT'mk_AC
Cuts at: 0 3650 4043 5514
Size: 3650 393 1471
Fragments arranged by size:
3650 1471 393
AclI AA'CG_TT
Cuts at: 0 2550 5514
Size: 2550 2964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 3006 5514
Size: 873 2133 2508
Fragments arranged by size:
2508 2133 873
AfeI AGC'GCT
Cuts at: 0 3406 5514
Size: 3406 2108
AflIII A'CryG_T
Cuts at: 0 3134 5514
Size: 3134 2380
AhdI GACnn_n'nnGTC
Cuts at: 0 4593 5514
Size: 4593 921
AleI CACnn'nnGTG
Cuts at: 0 744 1147 1180 3574 5362 5514
Size: 744 403 33 2394 1788 152
Fragments arranged by size:
2394 1788 744 403 152 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 762 1165 2131 3835 5230 5495 5514
Size: 762 403 966 1704 1395 265 19
Fragments arranged by size:
1704 1395 966 762 403 265 19
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 5514
Size: 1366 32 4116
Fragments arranged by size:
4116 1366 32
AlwNI CAG_nnn'CTG
Cuts at: 0 297 1526 2007 2297 4575 4719 5514
Size: 297 1229 481 290 2278 144 795
Fragments arranged by size:
2278 1229 795 481 297 290 144
ApaI G_GGCC'C
Cuts at: 0 21 211 920 1599 2440 4306 5514
Size: 21 190 709 679 841 1866 1208
Fragments arranged by size:
1866 1208 841 709 679 190 21
ApaLI G'TGCA_C
Cuts at: 0 231 955 3799 5514
Size: 231 724 2844 1715
Fragments arranged by size:
2844 1715 724 231
ApoI r'AATT_y
Cuts at: 0 3754 5514
Size: 3754 1760
AvaI C'yCGr_G
Cuts at: 0 1001 3341 3511 5333 5514
Size: 1001 2340 170 1822 181
Fragments arranged by size:
2340 1822 1001 181 170
BamHI G'GATC_C
Cuts at: 0 1241 2420 5273 5433 5514
Size: 1241 1179 2853 160 81
Fragments arranged by size:
2853 1241 1179 160 81
BanI G'GyrC_C
Cuts at: 0 49 829 997 1225 1270 2728 2749 3230
Size: 49 780 168 228 45 1458 21 481
Cuts at: 3230 3346 3397 4766 4932 4947 5514
Size: 116 51 1369 166 15 567
Fragments arranged by size:
1458 1369 780 567 481 228 168 166
116 51 49 45 21 15
BanII G_rGCy'C
Cuts at: 0 21 211 475 920 1072 1136 1599 2440
Size: 21 190 264 445 152 64 463 841
Cuts at: 2440 2770 3065 3452 4127 4259 4306 4561 5514
Size: 330 295 387 675 132 47 255 953
Fragments arranged by size:
953 841 675 463 445 387 330 295
264 255 190 152 132 64 47 21
BbeI G_GCGC'C
Cuts at: 0 4936 5514
Size: 4936 578
BbsI GAAGACnn'nnnn_
Cuts at: 0 675 684 761 879 2622 3153 3424 3843
Size: 675 9 77 118 1743 531 271 419
Cuts at: 3843 5024 5044 5514
Size: 1181 20 470
Fragments arranged by size:
1743 1181 675 531 470 419 271 118
77 20 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2226 2260 3354 3388 5514
Size: 2226 34 1094 34 2126
Fragments arranged by size:
2226 2126 1094 34 34
BclI T'GATC_A
Cuts at: 0 3890 5514
Size: 3890 1624
BglI GCCn_nnn'nGGC
Cuts at: 0 41 850 1516 3477 3924 5514
Size: 41 809 666 1961 447 1590
Fragments arranged by size:
1961 1590 809 666 447 41
BglII A'GATC_T
Cuts at: 0 4856 5231 5514
Size: 4856 375 283
Fragments arranged by size:
4856 375 283
BlpI GC'TnA_GC
Cuts at: 0 3592 5188 5514
Size: 3592 1596 326
Fragments arranged by size:
3592 1596 326
Bme1580I G_kGCm'C
Cuts at: 0 21 211 235 920 959 1228 1599 1980
Size: 21 190 24 685 39 269 371 381
Cuts at: 1980 2401 2440 2912 3233 3240 3349 3623 3803
Size: 421 39 472 321 7 109 274 180
Cuts at: 3803 4306 4891 4943 5514
Size: 503 585 52 571
Fragments arranged by size:
685 585 571 503 472 421 381 371
321 274 269 190 180 109 52 39
39 24 21 7
BmgBI CAC'GTC
Cuts at: 0 1817 2781 3585 3798 3885 5103 5514
Size: 1817 964 804 213 87 1218 411
Fragments arranged by size:
1817 1218 964 804 411 213 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1422 1995 2650 5514
Size: 1422 573 655 2864
Fragments arranged by size:
2864 1422 655 573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1305 4681 5514
Size: 1305 3376 833
Fragments arranged by size:
3376 1305 833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 799 1056 1361 1882 2821 2902 3681
Size: 208 591 257 305 521 939 81 779
Cuts at: 3681 3733 3742 4027 4105 4141 4150 4564 4765
Size: 52 9 285 78 36 9 414 201
Cuts at: 4765 5512 5514
Size: 747 2
Fragments arranged by size:
939 779 747 591 521 414 305 285
257 208 201 81 78 52 36 9
9 2
Bpu10I CC'TnA_GC
Cuts at: 0 373 1408 1639 3914 5514
Size: 373 1035 231 2275 1600
Fragments arranged by size:
2275 1600 1035 373 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 2104 4222 5062 5514
Size: 1231 873 2118 840 452
Fragments arranged by size:
2118 1231 873 840 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 1337 2892 3424 4692 5514
Size: 1337 1555 532 1268 822
Fragments arranged by size:
1555 1337 1268 822 532
BsaAI yAC'GTr
Cuts at: 0 1767 2103 3135 3273 4140 5514
Size: 1767 336 1032 138 867 1374
Fragments arranged by size:
1767 1374 1032 867 336 138
BsaBI GATnn'nnATC
Cuts at: 0 4216 5514
Size: 4216 1298
BsaHI Gr'CG_yC
Cuts at: 0 2486 2599 2627 2801 3308 4424 4551 4594
Size: 2486 113 28 174 507 1116 127 43
Cuts at: 4594 4933 5126 5514
Size: 339 193 388
Fragments arranged by size:
2486 1116 507 388 339 193 174 127
113 43 28
BsaWI w'CCGG_w
Cuts at: 0 566 5057 5514
Size: 566 4491 457
Fragments arranged by size:
4491 566 457
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1639 1669 2263 2293 2374 2404
Size: 754 30 855 30 594 30 81 30
Cuts at: 2404 2752 2782 3940 3970 4246 4276 4444 4474
Size: 348 30 1158 30 276 30 168 30
Cuts at: 4474 4597 4627 5332 5362 5514
Size: 123 30 705 30 152
Fragments arranged by size:
1158 855 754 705 594 348 276 168
152 123 81 30 30 30 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1266 1528 1660 1777 1879 3199 3739 4762
Size: 1266 262 132 117 102 1320 540 1023
Cuts at: 4762 4765 5443 5514
Size: 3 678 71
Fragments arranged by size:
1320 1266 1023 678 540 262 132 117
102 71 3
BseYI C'CCAG_C
Cuts at: 0 30 39 1116 1571 2014 3549 3707 4359
Size: 30 9 1077 455 443 1535 158 652
Cuts at: 4359 4691 5203 5514
Size: 332 512 311
Fragments arranged by size:
1535 1077 652 512 455 443 332 311
158 30 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 1407 1417 1630 2083 2197 3393 3481
Size: 768 639 10 213 453 114 1196 88
Cuts at: 3481 4057 4426 5151 5308 5471 5514
Size: 576 369 725 157 163 43
Fragments arranged by size:
1196 768 725 639 576 453 369 213
163 157 114 88 43 10
BsiEI CG_ry'CG
Cuts at: 0 1181 1190 2526 3846 3928 4780 4784 5514
Size: 1181 9 1336 1320 82 852 4 730
Fragments arranged by size:
1336 1320 1181 852 730 82 9 4
BsiHKAI G_wGCw'C
Cuts at: 0 235 475 553 959 1103 3803 4127 5514
Size: 235 240 78 406 144 2700 324 1387
Fragments arranged by size:
2700 1387 406 324 240 235 144 78
BsmI GAATG_Cn'
Cuts at: 0 159 5514
Size: 159 5355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2194 2425 2590 5514
Size: 2194 231 165 2924
Fragments arranged by size:
2924 2194 231 165
Bsp1286I G_dGCh'C
Cuts at: 0 21 211 235 475 553 920 959 1072
Size: 21 190 24 240 78 367 39 113
Cuts at: 1072 1103 1136 1228 1599 1980 2401 2440 2770
Size: 31 33 92 371 381 421 39 330
Cuts at: 2770 2912 3065 3233 3240 3349 3452 3623 3803
Size: 142 153 168 7 109 103 171 180
Cuts at: 3803 4127 4259 4306 4561 4891 4943 5514
Size: 324 132 47 255 330 52 571
Fragments arranged by size:
571 421 381 371 367 330 330 324
255 240 190 180 171 168 153 142
132 113 109 103 92 78 52 47
39 39 33 31 24 21 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 37 186 797 841 1112 1429 1880 1915
Size: 37 149 611 44 271 317 451 35
Cuts at: 1915 1928 2348 3309 3515 3971 4163 4727 4962
Size: 13 420 961 206 456 192 564 235
Cuts at: 4962 5280 5514
Size: 318 234
Fragments arranged by size:
961 611 564 456 451 420 318 317
271 235 234 206 192 149 44 37
35 13
BsrBI CCG'CTC
Cuts at: 0 3591 4836 5055 5514
Size: 3591 1245 219 459
Fragments arranged by size:
3591 1245 459 219
BsrDI GCAATG_nn'
Cuts at: 0 444 525 5514
Size: 444 81 4989
Fragments arranged by size:
4989 444 81
BsrFI r'CCGG_y
Cuts at: 0 622 1392 1589 2133 2521 3808 3895 3927
Size: 622 770 197 544 388 1287 87 32
Cuts at: 3927 4610 5514
Size: 683 904
Fragments arranged by size:
1287 904 770 683 622 544 388 197
87 32
BsrGI T'GTAC_A
Cuts at: 0 4262 5514
Size: 4262 1252
BssHII G'CGCG_C
Cuts at: 0 366 5514
Size: 366 5148
BssSI C'ACGA_G
Cuts at: 0 1930 2041 3802 5113 5362 5514
Size: 1930 111 1761 1311 249 152
Fragments arranged by size:
1930 1761 1311 249 152 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1430 1550 1886 2297 2306 4719 4773 4947
Size: 1430 120 336 411 9 2413 54 174
Cuts at: 4947 5286 5514
Size: 339 228
Fragments arranged by size:
2413 1430 411 339 336 228 174 120
54 9
BstEII G'GTnAC_C
Cuts at: 0 490 2136 2787 5514
Size: 490 1646 651 2727
Fragments arranged by size:
2727 1646 651 490
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1860 2022 3117 5514
Size: 1860 162 1095 2397
Fragments arranged by size:
2397 1860 1095 162
BstYI r'GATC_y
Cuts at: 0 1241 2420 2510 4571 4856 5231 5273 5433
Size: 1241 1179 90 2061 285 375 42 160
Cuts at: 5433 5514
Size: 81
Fragments arranged by size:
2061 1241 1179 375 285 160 90 81
42
Bsu36I CC'TnA_GG
Cuts at: 0 58 126 1958 3182 5514
Size: 58 68 1832 1224 2332
Fragments arranged by size:
2332 1832 1224 68 58
BtgI C'CryG_G
Cuts at: 0 203 476 848 1035 1504 2745 3044 3085
Size: 203 273 372 187 469 1241 299 41
Cuts at: 3085 3623 3919 4497 5514
Size: 538 296 578 1017
Fragments arranged by size:
1241 1017 578 538 469 372 299 296
273 203 187 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3039 3379 3621 5127 5514
Size: 3039 340 242 1506 387
Fragments arranged by size:
3039 1506 387 340 242
BtsI GCAGTG_nn'
Cuts at: 0 4 799 915 2115 4891 5514
Size: 4 795 116 1200 2776 623
Fragments arranged by size:
2776 1200 795 623 116 4
DraIII CAC_nnn'GTG
Cuts at: 0 1430 4773 4947 5118 5514
Size: 1430 3343 174 171 396
Fragments arranged by size:
3343 1430 396 174 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 1969 3154 4537 5107 5514
Size: 1969 1185 1383 570 407
Fragments arranged by size:
1969 1383 1185 570 407
EaeI y'GGCC_r
Cuts at: 0 200 431 452 839 1056 1178 1236 1556
Size: 200 231 21 387 217 122 58 320
Cuts at: 1556 1769 2286 2519 2523 2991 3925 4145 4777
Size: 213 517 233 4 468 934 220 632
Cuts at: 4777 5514
Size: 737
Fragments arranged by size:
934 737 632 517 468 387 320 233
231 220 217 213 200 122 58 21
4
EagI C'GGCC_G
Cuts at: 0 1178 2523 3925 4777 5514
Size: 1178 1345 1402 852 737
Fragments arranged by size:
1402 1345 1178 852 737
EarI CTCTTCn'nnn_
Cuts at: 0 349 3661 4120 4276 5514
Size: 349 3312 459 156 1238
Fragments arranged by size:
3312 1238 459 349 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 379 5455 5514
Size: 379 5076 59
Fragments arranged by size:
5076 379 59
EcoICRI GAG'CTC
Cuts at: 0 473 4125 5514
Size: 473 3652 1389
Fragments arranged by size:
3652 1389 473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 799 873 1056 1361 1882 2821 2902
Size: 208 591 74 183 305 521 939 81
Cuts at: 2902 3006 3681 3733 3742 4027 4105 4141 4150
Size: 104 675 52 9 285 78 36 9
Cuts at: 4150 4564 4765 5512 5514
Size: 414 201 747 2
Fragments arranged by size:
939 747 675 591 521 414 305 285
208 201 183 104 81 78 74 52
36 9 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 1642 4675 4744 4747 5514
Size: 346 1296 3033 69 3 767
Fragments arranged by size:
3033 1296 767 346 69 3
EcoO109I rG'GnC_Cy
Cuts at: 0 18 62 481 801 916 1446 1917 2011
Size: 18 44 419 320 115 530 471 94
Cuts at: 2011 2156 2373 2437 2900 3260 3717 3942 4302
Size: 145 217 64 463 360 457 225 360
Cuts at: 4302 4440 4716 5514
Size: 138 276 798
Fragments arranged by size:
798 530 471 463 457 419 360 360
320 276 225 217 145 138 115 94
64 44 18
EcoRI G'AATT_C
Cuts at: 0 3754 5514
Size: 3754 1760
EcoRV GAT'ATC
Cuts at: 0 1044 5514
Size: 1044 4470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 3177 4062 4986 5514
Size: 93 3084 885 924 528
Fragments arranged by size:
3084 924 885 528 93
FseI GG_CCGG'CC
Cuts at: 0 2525 5514
Size: 2525 2989
FspI TGC'GCA
Cuts at: 0 4171 5514
Size: 4171 1343
HaeII r_GCGC'y
Cuts at: 0 324 1571 3408 4072 4734 4936 5514
Size: 324 1247 1837 664 662 202 578
Fragments arranged by size:
1837 1247 664 662 578 324 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 1258 1290 1790 1822 2752 2784
Size: 109 32 1117 32 500 32 930 32
Cuts at: 2784 3082 3114 4444 4476 4579 4611 4789 4821
Size: 298 32 1330 32 103 32 178 32
Cuts at: 4821 5514
Size: 693
Fragments arranged by size:
1330 1117 930 693 500 298 178 109
103 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 288 3324 4626 5514
Size: 288 3036 1302 888
Fragments arranged by size:
3036 1302 888 288
HindIII A'AGCT_T
Cuts at: 0 2976 5514
Size: 2976 2538
KasI G'GCGC_C
Cuts at: 0 4932 5514
Size: 4932 582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 2700 3983 4229 4559 5514
Size: 542 2158 1283 246 330 955
Fragments arranged by size:
2158 1283 955 542 330 246
MscI TGG'CCA
Cuts at: 0 202 433 454 4147 5514
Size: 202 231 21 3693 1367
Fragments arranged by size:
3693 1367 231 202 21
MslI CAynn'nnrTG
Cuts at: 0 544 744 1147 1180 3115 3574 3640 3817
Size: 544 200 403 33 1935 459 66 177
Cuts at: 3817 4432 4633 4643 5362 5514
Size: 615 201 10 719 152
Fragments arranged by size:
1935 719 615 544 459 403 201 200
177 152 66 33 10
MspA1I CmG'CkG
Cuts at: 0 168 186 294 413 820 945 1116 1506
Size: 168 18 108 119 407 125 171 390
Cuts at: 1506 1614 1901 1994 3087 3609 3711 3743 3921
Size: 108 287 93 1093 522 102 32 178
Cuts at: 3921 4017 4602 5457 5485 5514
Size: 96 585 855 28 29
Fragments arranged by size:
1093 855 585 522 407 390 287 178
171 168 125 119 108 108 102 96
93 32 29 28 18
NaeI GCC'GGC
Cuts at: 0 624 1591 2523 3810 5514
Size: 624 967 932 1287 1704
Fragments arranged by size:
1704 1287 967 932 624
NarI GG'CG_CC
Cuts at: 0 4933 5514
Size: 4933 581
NcoI C'CATG_G
Cuts at: 0 3044 3623 5514
Size: 3044 579 1891
Fragments arranged by size:
3044 1891 579
NgoMIV G'CCGG_C
Cuts at: 0 622 1589 2521 3808 5514
Size: 622 967 932 1287 1706
Fragments arranged by size:
1706 1287 967 932 622
NruI TCG'CGA
Cuts at: 0 3539 5514
Size: 3539 1975
NspI r_CATG'y
Cuts at: 0 747 5110 5514
Size: 747 4363 404
Fragments arranged by size:
4363 747 404
PasI CC'CwG_GG
Cuts at: 0 1599 2442 5514
Size: 1599 843 3072
Fragments arranged by size:
3072 1599 843
PflMI CCAn_nnn'nTGG
Cuts at: 0 1526 2007 4005 5118 5514
Size: 1526 481 1998 1113 396
Fragments arranged by size:
1998 1526 1113 481 396
PfoI T'CCnGG_A
Cuts at: 0 4118 4127 4541 4649 4742 4979 5009 5514
Size: 4118 9 414 108 93 237 30 505
Fragments arranged by size:
4118 505 414 237 108 93 30 9
PmlI CAC'GTG
Cuts at: 0 1767 2103 5514
Size: 1767 336 3411
Fragments arranged by size:
3411 1767 336
PpuMI rG'GwC_Cy
Cuts at: 0 481 801 1446 1917 2011 2156 2900 4440
Size: 481 320 645 471 94 145 744 1540
Cuts at: 4440 4716 5514
Size: 276 798
Fragments arranged by size:
1540 798 744 645 481 471 320 276
145 94
PshAI GACnn'nnGTC
Cuts at: 0 1994 5514
Size: 1994 3520
PspOMI G'GGCC_C
Cuts at: 0 17 207 916 1595 2436 4302 5514
Size: 17 190 709 679 841 1866 1212
Fragments arranged by size:
1866 1212 841 709 679 190 17
PstI C_TGCA'G
Cuts at: 0 11 185 794 1157 2302 3071 3107 3377
Size: 11 174 609 363 1145 769 36 270
Cuts at: 3377 4898 4959 5474 5514
Size: 1521 61 515 40
Fragments arranged by size:
1521 1145 769 609 515 363 270 174
61 40 36 11
PvuII CAG'CTG
Cuts at: 0 168 186 294 1614 1994 3711 5457 5514
Size: 168 18 108 1320 380 1717 1746 57
Fragments arranged by size:
1746 1717 1320 380 168 108 57 18
SacI G_AGCT'C
Cuts at: 0 475 4127 5514
Size: 475 3652 1387
Fragments arranged by size:
3652 1387 475
SacII CC_GC'GG
Cuts at: 0 1507 3088 3922 5514
Size: 1507 1581 834 1592
Fragments arranged by size:
1592 1581 1507 834
SanDI GG'GwC_CC
Cuts at: 0 801 2011 2900 5514
Size: 801 1210 889 2614
Fragments arranged by size:
2614 1210 889 801
SapI GCTCTTCn'nnn_
Cuts at: 0 3661 4120 4276 5514
Size: 3661 459 156 1238
Fragments arranged by size:
3661 1238 459 156
ScaI AGT'ACT
Cuts at: 0 4090 4966 5401 5514
Size: 4090 876 435 113
Fragments arranged by size:
4090 876 435 113
SexAI A'CCwGG_T
Cuts at: 0 1328 4487 4769 5514
Size: 1328 3159 282 745
Fragments arranged by size:
3159 1328 745 282
SfcI C'TryA_G
Cuts at: 0 7 181 790 1153 2298 2328 3067 3103
Size: 7 174 609 363 1145 30 739 36
Cuts at: 3103 3373 4894 4955 5470 5514
Size: 270 1521 61 515 44
Fragments arranged by size:
1521 1145 739 609 515 363 270 174
61 44 36 30 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1516 5514
Size: 1516 3998
SfoI GGC'GCC
Cuts at: 0 4934 5514
Size: 4934 580
SmaI CCC'GGG
Cuts at: 0 1003 3343 5335 5514
Size: 1003 2340 1992 179
Fragments arranged by size:
2340 1992 1003 179
SmlI C'TyrA_G
Cuts at: 0 1246 2119 4237 5077 5514
Size: 1246 873 2118 840 437
Fragments arranged by size:
2118 1246 873 840 437
StuI AGG'CCT
Cuts at: 0 124 586 1166 3196 3388 5514
Size: 124 462 580 2030 192 2126
Fragments arranged by size:
2126 2030 580 462 192 124
StyI C'CwwG_G
Cuts at: 0 3044 3152 3623 3866 4037 4676 5225 5423
Size: 3044 108 471 243 171 639 549 198
Cuts at: 5423 5514
Size: 91
Fragments arranged by size:
3044 639 549 471 243 198 171 108
91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1441 2434 2767 3385 3448 3534 4798 5055
Size: 1441 993 333 618 63 86 1264 257
Cuts at: 5055 5514
Size: 459
Fragments arranged by size:
1441 1264 993 618 459 333 257 86
63
TatI w'GTAC_w
Cuts at: 0 3440 4088 4262 4334 4964 5399 5514
Size: 3440 648 174 72 630 435 115
Fragments arranged by size:
3440 648 630 435 174 115 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 778 810 2562 2594 2726 2758 4597 4629
Size: 778 32 1752 32 132 32 1839 32
Cuts at: 4629 5332 5364 5514
Size: 703 32 150
Fragments arranged by size:
1839 1752 778 703 150 132 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 283 3847 5514
Size: 283 3564 1667
Fragments arranged by size:
3564 1667 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 462 2845 3117 4495 5514
Size: 321 141 2383 272 1378 1019
Fragments arranged by size:
2383 1378 1019 321 272 141
XmaI C'CCGG_G
Cuts at: 0 1001 3341 5333 5514
Size: 1001 2340 1992 181
Fragments arranged by size:
2340 1992 1001 181
XmnI GAAnn'nnTTC
Cuts at: 0 424 3754 5514
Size: 424 3330 1760
Fragments arranged by size:
3330 1760 424
ZraI GAC'GTC
Cuts at: 0 2628 4595 5514
Size: 2628 1967 919
Fragments arranged by size:
2628 1967 919
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AgeI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BfrBI BmtI BsiWI BspEI BspHI BstBI
BstZ17I ClaI CspCI DraI FspAI HpaI KpnI MfeI
MluI NdeI NheI NotI NsiI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SbfI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI