HUGE

Gene/Protein Characteristic Table for KIAA1149

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK04248
Accession No. :	AB032975
Description :	Beta-secretase 1 precursor.
HUGO Gene Name :
Clone Name :	fg04087 [Vector Info]
Source :	Human fetal brain
Note :	We replaced hg01289, former representative clones for KIAA1149 with fg04087. (2001/5/29)

Features of the cloned DNA sequence	Description

Length: 5814 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3865 bp

Genome contig ID gi51511727r_116561627
PolyA signal sequence
(AGTAAA,-23)
+----*----+----*----+----*----+----
GAGCGTTAACACAGTAAAATATTCAATAAGAAGTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAACTACTAGGGTTACTTTTTTTTCCTCAATCAGAGTCTCATTTTATCCA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 11 r 116661627 116692163 9 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 532 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
P56817	5.9e-191	96.3	Beta-secretase ...	Homo sapiens
AAF13715	1.4e-190	97.3	memapsin 2 [Hom...	Homo sapiens
AAH36084	1.9e-190	96.1	Beta-site APP-c...	Homo sapiens
ACA05927	2.9e-190	96.1	beta-secretase ...	Homo sapiens
XP_001094263	3.8e-190	95.9	beta-site APP-c...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR009120 88 114 PR01816 Peptidase A1

IPR009119 94 113 PR01815 Peptidase A1

IPR001461 112 132 PR00792 Peptidase A1

IPR009119 124 152 PR01815 Peptidase A1

IPR001461 264 277 PR00792 Peptidase A1

IPR009119 299 323 PR01815 Peptidase A1

IPR001461 317 328 PR00792 Peptidase A1

IPR009120 398 407 PR01816 Peptidase A1

IPR001461 423 438 PR00792 Peptidase A1

IPR009120 452 463 PR01816 Peptidase A1

HMMPfam IPR001461 105 449 PF00026 Peptidase A1

ScanRegExp IPR001969 121 132 PS00141 Peptidase aspartic

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR009120	88	114	PR01816	Peptidase A1
IPR009119	94	113	PR01815	Peptidase A1
IPR001461	112	132	PR00792	Peptidase A1
IPR009119	124	152	PR01815	Peptidase A1
IPR001461	264	277	PR00792	Peptidase A1
IPR009119	299	323	PR01815	Peptidase A1
IPR001461	317	328	PR00792	Peptidase A1
IPR009120	398	407	PR01816	Peptidase A1
IPR001461	423	438	PR00792	Peptidase A1
IPR009120	452	463	PR01816	Peptidase A1
HMMPfam	IPR001461	105	449	PF00026	Peptidase A1
ScanRegExp	IPR001969	121	132	PS00141	Peptidase aspartic

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 487 MTIAYVMAAICALFMLPLCLMVC 509 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	487	MTIAYVMAAICALFMLPLCLMVC	509	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCTAGCTCGGAACTTACTGTG

Primer_r
: ATAATAGGCTGATGGGACTTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 11

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GAAAATAGGGTGGACAGAAGC
Primer_r	: TCACAGTCCGAGAATAACAAC
PCR product length	: 77 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3865 bp
Genome contig ID	gi51511727r_116561627
PolyA signal sequence (AGTAAA,-23)	+--------+--------+----*----+---- GAGCGTTAACACAGTAAAATATTCAATAAGAAGTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACTACTAGGGTTACTTTTTTTTCCTCAATCAGAGTCTCATTTTATCCA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	11	r	116661627	116692163	9	99.2	Perfect prediction

Primer_f	: TCTAGCTCGGAACTTACTGTG
Primer_r	: ATAATAGGCTGATGGGACTTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1149

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information