################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AclI AgeI AhdI AscI AspEI AspI AvrII BbrPI BcgI BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI BsmI BspDI BssHII BssSI BstBI CelII ClaI CpoI Eam1105I EcoRI EcoRV EcoT22I FseI KspI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SphI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflII 4560 CTTAAG C^TTAAG AflIII 4946 ACATGT A^C[GA][CT]GT Alw44I 1707 GTGCAC G^TGCAC Aor51HI 1695 AGCGCT AGC^GCT ApaLI 1707 GTGCAC G^TGCAC AseI 2812 ATTAAT AT^TAAT AsnI 2812 ATTAAT AT^TAAT AviII 531 TGCGCA TGC^GCA BamHI 220 GGATCC G^GATCC BfrI 4560 CTTAAG C^TTAAG BglII 3132 AGATCT A^GATCT BsgI 3735 CTGCAC GTGCAG(16/14) BsiEI 605 CGGCCG CG[GA][CT]^CG Bsp1407I 1174 TGTACA T^GTACA BspLU11I 4946 ACATGT A^CATGT BsrFI 129 ACCGGC [GA]^CCGG[CT] BsrGI 1174 TGTACA T^GTACA BstXI 5581 CCAAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 129 ACCGGC [GA]^CCGG[CT] DrdI 1790 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 605 CGGCCG C^GGCCG EclXI 605 CGGCCG C^GGCCG Eco47III 1695 AGCGCT AGC^GCT Eco52I 605 CGGCCG C^GGCCG EcoNI 812 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 531 TGCGCA TGC^GCA NdeI 2852 CATATG CA^TATG PflMI 1447 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4787 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2812 ATTAAT AT^TAAT SbfI 306 CCTGCAGG CCTGCA^GG SfiI 303 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 4560 CTTAAG C^T[CT][GA]AG Sse8387I 306 CCTGCAGG CCTGCA^GG SspBI 1174 TGTACA T^GTACA StuI 1008 AGGCCT AGG^CCT Van91I 1447 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1193 GTATAC GT^[AC][GT]AC 3531 GTATAC AccIII 217 TCCGGA T^CCGGA 814 TCCGGA Asp700I 1457 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5137 GAAAAGATTC BbeI 383 GGCGCC GGCGC^C 549 GGCGCC BbvCI 3653 CCTCAGC CCTCAGC(-5/-2) 5097 GCTGAGG BciVI 719 GGATAC GTATCC(6/5) 4500 GGATAC BclI 1147 TGATCA T^GATCA 1228 TGATCA BsaAI 669 TACGTG [CT]AC^GT[GA] 3410 TACGTG BsaWI 217 TCCGGA [AT]^CCGG[AT] 814 TCCGGA BseAI 217 TCCGGA T^CCGGA 814 TCCGGA BspEI 217 TCCGGA T^CCGGA 814 TCCGGA BspHI 1807 TCATGA T^CATGA 4641 TCATGA BsrBI 267 CCGCTC CCGCTC(-3/-3) 3397 CCGCTC Bst1107I 1193 GTATAC GTA^TAC 3531 GTATAC BstZ17I 1193 GTATAC GTA^TAC 3531 GTATAC DraIII 3211 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3738 CACTTGGTG FbaI 1147 TGATCA T^GATCA 1228 TGATCA HindIII 2369 AAGCTT A^AGCTT 5666 AAGCTT HpaI 5053 GTTAAC GTT^AAC 5784 GTTAAC KasI 383 GGCGCC G^GCGCC 549 GGCGCC MroI 217 TCCGGA T^CCGGA 814 TCCGGA NarI 383 GGCGCC GG^CGCC 549 GGCGCC NspI 4195 GCATGT [GA]CATG^[CT] 4946 ACATGT RcaI 1807 TCATGA T^CATGA 4641 TCATGA ScaI 2303 AGTACT AGT^ACT 4718 AGTACT SfoI 383 GGCGCC GGC^GCC 549 GGCGCC SspI 5746 AATATT AAT^ATT 5797 AATATT TaqI 1162 TCGA T^CGA 3958 TCGA TthHB8I 1162 TCGA T^CGA 3958 TCGA XmnI 1457 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5137 GAAAAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI