Gene/Protein Characteristic Table for KIAA0144
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Product ID ORK00028
Accession No D63478
Description ubiquitin associated protein 2-like, transcript variant 2
Clone name ha03843
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (3411 bp)
Predicted protein sequence (983 aa)
Flexi ORF Clone FXC00028
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0144 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3411 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 353 bp
Genome contig ID gi89161185f_152359279
PolyA signal sequence
(AATAAA,-28)
+----*----+----*----+----*----+----
AGTCTTCAATAAACTGTGGTATTTCTTTAGCTAAC
Flanking genome sequence
(143328 - 143377)
----+----*----+----*----+----*----+----*----+----*
TCTGGCGTTCTTTCCGTGCATGTCTTTGTGGACACTAGGAACCAATGCTG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 152459279 152502605 25 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 983 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001113998 0 99.9 ubiquitin assoc...
Macaca mulatta
AAI48080 0 98.7 UBAP2L protein ...
Bos taurus
ABM06060 0 98.7 ubiquitin assoc...
Bos taurus
BAG64560 0 99.1 unnamed protein...
Homo sapiens
EDL15170 0 98.1 ubiquitin assoc...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB040924 9e-26 47.1 KIAA1491
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000449 49 89 PF00627 Ubiquitin-associated/Translation elongation factor EF1B
HMMSmart IPR000449 50 88 SM00165 Ubiquitin-associated/Translation elongation factor EF1B
ProfileScan IPR000449 49 89 PS50030 Ubiquitin-associated/Translation elongation factor EF1B
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 12
Experimental conditions
Panel name Genebridge 4
Primer_f ACTTAAACTCCCACCTACTCC
Primer_r TGAGGTTGTTCCCAGTTTCCC
PCR product length 147 bp
PCR conditions 95 °C15 sec64 °C120 sec30 cycles
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