##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AocI Aor51HI AscI
AspI AvrII BamHI BbeI BbrPI
BfrI BglI BlnI BsaAI BsaHI
BseAI BsiWI BspEI BspLU11I BsrBI
BssHII BssSI BstBI BstEII BstPI
Bsu36I CpoI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRI EcoRV FseI
Hin1I HindIII KasI KspI MaeII
MluI MroI NaeI NarI NgoMIV
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SapI SbfI SexAI SfiI SfoI
SfuI SmaI SnaBI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1051 GGTACC G^GTACC
AhdI 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1128 GTGCAC G^TGCAC
ApaI 922 GGGCCC GGGCC^C
ApaLI 1128 GTGCAC G^TGCAC
Asp718I 1051 GGTACC G^GTACC
AspEI 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 161 CTCGGG C^[CT]CG[GA]G
AviII 3124 TGCGCA TGC^GCA
BbvCI 81 GCTGAGG CCTCAGC(-5/-2)
BciVI 1378 GTATCC GTATCC(6/5)
BsaBI 833 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3489 GAGACC GGTCTC(1/5)
BsaWI 11 ACCGGA [AT]^CCGG[AT]
BsmBI 1971 CGTCTC CGTCTC(1/5)
BsmI 3523 GAATGC GAATGC(1/-1)
BsoBI 161 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2641 TGTACA T^GTACA
BspDI 2690 ATCGAT AT^CGAT
BspHI 132 TCATGA T^CATGA
BsrFI 53 GCCGGT [GA]^CCGG[CT]
BsrGI 2641 TGTACA T^GTACA
Bst1107I 1291 GTATAC GTA^TAC
BstZ17I 1291 GTATAC GTA^TAC
Cfr10I 53 GCCGGT [GA]^CCGG[CT]
ClaI 2690 ATCGAT AT^CGAT
DraI 3377 TTTAAA TTT^AAA
DraII 922 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 922 GGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 3558 CCCGC CCCGC(4/6)
FspI 3124 TGCGCA TGC^GCA
HaeII 885 AGCGCC [GA]GCGC^[CT]
HpaI 1147 GTTAAC GTT^AAC
KpnI 1051 GGTACC GGTAC^C
MamI 833 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3186 CATATG CA^TATG
NheI 230 GCTAGC G^CTAGC
NotI 92 GCGGCCGC GC^GGCCGC
PflMI 3263 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 132 TCATGA T^CATGA
SacI 1689 GAGCTC GAGCT^C
SalI 2880 GTCGAC G^TCGAC
SgrAI 52 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 2292 CTCAAG C^T[CT][GA]AG
SpeI 1124 ACTAGT A^CTAGT
SphI 1599 GCATGC GCATG^C
SspBI 2641 TGTACA T^GTACA
Van91I 3263 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1291 GTATAC GT^[AC][GT]AC
2880 GTCGAC
AseI 1702 ATTAAT AT^TAAT
2395 ATTAAT
AsnI 1702 ATTAAT AT^TAAT
2395 ATTAAT
BanII 922 GGGCCC G[GA]GC[CT]^C
1689 GAGCTC
BbsI 2888 GAAGAC GAAGAC(2/6)
354 GTCTTC
BcgI 1471 GCAGGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3145 GCACAAGATTCG
BclI 1639 TGATCA T^GATCA
2563 TGATCA
BcnI 77 CCCGG CC^[GC]GG
1667 CCCGG
BpuAI 2888 GAAGAC GAAGAC(2/6)
354 GTCTTC
BsgI 2770 CTGCAC GTGCAG(16/14)
512 GTGCAG
BsrDI 998 GCAATG GCAATG(2/0)
2958 GCAATG
BstXI 2728 CCATCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3084 CCAACTATGTGG
DsaI 1056 CCATGG C^C[GA][CT]GG
1367 CCATGG
EarI 927 CTCTTC CTCTTC(1/4)
3171 CTCTTC
EcoNI 701 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1484 CCTGGAGCAGG
EcoT22I 1173 ATGCAT ATGCA^T
2650 ATGCAT
FbaI 1639 TGATCA T^GATCA
2563 TGATCA
Ksp632I 927 CTCTTC CTCTTC(1/4)
3171 CTCTTC
MfeI 1821 CAATTG C^AATTG
3252 CAATTG
MslI 2729 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3061 CACCATGATG
MunI 1821 CAATTG C^AATTG
3252 CAATTG
NciI 77 CCCGG CC^[GC]GG
1667 CCCGG
NcoI 1056 CCATGG C^CATGG
1367 CCATGG
NsiI 1173 ATGCAT ATGCA^T
2650 ATGCAT
NspI 1599 GCATGC [GA]CATG^[CT]
2863 GCATGT
PshBI 1702 ATTAAT AT^TAAT
2395 ATTAAT
ScaI 899 AGTACT AGT^ACT
1615 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BbvI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII EaeI EagI
EclXI Eco52I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI