################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AocI Aor51HI AscI AspI AvrII BamHI BbeI BbrPI BfrI BglI BlnI BsaAI BsaHI BseAI BsiWI BspEI BspLU11I BsrBI BssHII BssSI BstBI BstEII BstPI Bsu36I CpoI DraIII DrdI Eco47III Eco81I EcoO65I EcoRI EcoRV FseI Hin1I HindIII KasI KspI MaeII MluI MroI NaeI NarI NgoMIV NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SapI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1051 GGTACC G^GTACC AhdI 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1128 GTGCAC G^TGCAC ApaI 922 GGGCCC GGGCC^C ApaLI 1128 GTGCAC G^TGCAC Asp718I 1051 GGTACC G^GTACC AspEI 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 161 CTCGGG C^[CT]CG[GA]G AviII 3124 TGCGCA TGC^GCA BbvCI 81 GCTGAGG CCTCAGC(-5/-2) BciVI 1378 GTATCC GTATCC(6/5) BsaBI 833 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3489 GAGACC GGTCTC(1/5) BsaWI 11 ACCGGA [AT]^CCGG[AT] BsmBI 1971 CGTCTC CGTCTC(1/5) BsmI 3523 GAATGC GAATGC(1/-1) BsoBI 161 CTCGGG C^[CT]CG[GA]G Bsp1407I 2641 TGTACA T^GTACA BspDI 2690 ATCGAT AT^CGAT BspHI 132 TCATGA T^CATGA BsrFI 53 GCCGGT [GA]^CCGG[CT] BsrGI 2641 TGTACA T^GTACA Bst1107I 1291 GTATAC GTA^TAC BstZ17I 1291 GTATAC GTA^TAC Cfr10I 53 GCCGGT [GA]^CCGG[CT] ClaI 2690 ATCGAT AT^CGAT DraI 3377 TTTAAA TTT^AAA DraII 922 GGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 2274 GACATATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 922 GGGCCCT [GA]G^G[ACGT]CC[CT] FauI 3558 CCCGC CCCGC(4/6) FspI 3124 TGCGCA TGC^GCA HaeII 885 AGCGCC [GA]GCGC^[CT] HpaI 1147 GTTAAC GTT^AAC KpnI 1051 GGTACC GGTAC^C MamI 833 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 3186 CATATG CA^TATG NheI 230 GCTAGC G^CTAGC NotI 92 GCGGCCGC GC^GGCCGC PflMI 3263 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 132 TCATGA T^CATGA SacI 1689 GAGCTC GAGCT^C SalI 2880 GTCGAC G^TCGAC SgrAI 52 CGCCGGTG C[GA]^CCGG[CT]G SmlI 2292 CTCAAG C^T[CT][GA]AG SpeI 1124 ACTAGT A^CTAGT SphI 1599 GCATGC GCATG^C SspBI 2641 TGTACA T^GTACA Van91I 3263 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1291 GTATAC GT^[AC][GT]AC 2880 GTCGAC AseI 1702 ATTAAT AT^TAAT 2395 ATTAAT AsnI 1702 ATTAAT AT^TAAT 2395 ATTAAT BanII 922 GGGCCC G[GA]GC[CT]^C 1689 GAGCTC BbsI 2888 GAAGAC GAAGAC(2/6) 354 GTCTTC BcgI 1471 GCAGGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3145 GCACAAGATTCG BclI 1639 TGATCA T^GATCA 2563 TGATCA BcnI 77 CCCGG CC^[GC]GG 1667 CCCGG BpuAI 2888 GAAGAC GAAGAC(2/6) 354 GTCTTC BsgI 2770 CTGCAC GTGCAG(16/14) 512 GTGCAG BsrDI 998 GCAATG GCAATG(2/0) 2958 GCAATG BstXI 2728 CCATCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3084 CCAACTATGTGG DsaI 1056 CCATGG C^C[GA][CT]GG 1367 CCATGG EarI 927 CTCTTC CTCTTC(1/4) 3171 CTCTTC EcoNI 701 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1484 CCTGGAGCAGG EcoT22I 1173 ATGCAT ATGCA^T 2650 ATGCAT FbaI 1639 TGATCA T^GATCA 2563 TGATCA Ksp632I 927 CTCTTC CTCTTC(1/4) 3171 CTCTTC MfeI 1821 CAATTG C^AATTG 3252 CAATTG MslI 2729 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3061 CACCATGATG MunI 1821 CAATTG C^AATTG 3252 CAATTG NciI 77 CCCGG CC^[GC]GG 1667 CCCGG NcoI 1056 CCATGG C^CATGG 1367 CCATGG NsiI 1173 ATGCAT ATGCA^T 2650 ATGCAT NspI 1599 GCATGC [GA]CATG^[CT] 2863 GCATGT PshBI 1702 ATTAAT AT^TAAT 2395 ATTAAT ScaI 899 AGTACT AGT^ACT 1615 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BbvI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EaeI EagI EclXI Eco52I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI