PLCH2 ORF clone pFN21AF0417

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Halotag ORF Clone pFN21AF0417

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Clone info.

Species

human

Clone Name

pFN21AF0417 Sequence (with vector) Restriction Map

Product ID

FHC05198

Flexi Clone PID

FXC05198

ORF Sequence

DNA sequence (1617 bp)
Protein sequence (539 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527145]

Gene

FLJ00417

Symbol

PLCH2

Alias : PLC-L4, PLC-eta2, PLCL4, PLCeta2

Description

phospholipase C, eta 2

Original Clone ID

sj06336s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1617 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 539 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS59959.1 1.8e-42 54.9 PLCH2 CCDS33881.1 2.7e-20 58.8 PLCH1 CCDS46940.1 3.2e-15 53.6 PLCH1 CCDS46939.1 4.6e-15 53.6 PLCH1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000008 109 179 PF00168 C2 calcium-dependent membrane targeting HMMSmart IPR000008 85 195 SM00239 C2 calcium-dependent membrane targeting ProfileScan IPR018029 114 180 PS50004 C2 membrane targeting protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055453 1416 612836 54.9 83.3 XP_016858362 1475 612836 54.9 83.3 XP_016858361 1511 612836 54.9 83.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AF0417 Download

>FLJ00417  1617 bp
ATGATGGCTCCCCCGACAGCCGGCCCCCTTCCTGGCCCAGCTCTTCCGCCTGAGGACCCA
GGGCCGGATCCGGAGAGCAGGTGGCTTTTCTTGAGCGCCAACATTCTGCCCGTGGTGGAG
CGGTGCATGGGTGCCATGCAAGAGGGGATGCAGATGGTGAAGCTGCGTGGCGGCTCCAAG
GGCCTGGTCCGCTTCTACTACCTGGACGAGCACCGCTCCTGCATCCGCTGGAGGCCCTCA
CGCAAGAACGAGAAGGCCAAGATCTCCATCGACTCCATCCAGGAGGTGAGTGAGGGGCGG
CAGTCGGAGGTCTTCCAGCGCTACCCTGACGGCAGCTTCGACCCCTTTGTGGAGGTGGAG
ATCATTGGGCTCCCTGTGGACTGCAGCAGGGAGCAGACCCGCGTGGTGGACGACAACGGG
TTCAACCCCACCTGGGAGGAGACCCTGGTTTTCATGGTGCACATGCCGGAGATCGCGCTG
GTCCGCTTCCTCGTCTGGGACCACGATCCCATTGGGCGTGACTTCATTGGCCAGAGGACG
CTGGCCTTCAGCAGCATGATGCCAGGCTACAGACACGTGTACCTAGAAGGGATGGAAGAG
GCCTCCATCTTCGTGCATGTGGCTGTCAGTGACATCAGCGGTAAGGTCAAGCAGGCTCTG
GGCCTAAAAGGCCTCTTCCTCCGAGGCCCAAAGCCCGGCTCGCTGGACAGTCATGCTGCT
GGGCGGCCCCCGGCCCGGCCCTCCGTTAGCCAGCGGATCCTGCGGCGCACGGCCAGCGCC
CCGACCAAGAGCCAGAAGCCGGGCCGCAGGGGCTTCCCGGAGCTGGTCCTGGGTACACGG
GACACAGGCTCCAAGGGGGTGGCAGACGATGTGGTGCCCCCCGGGCCCGGACCTGCTCCG
GAAGCCCCAGCCCAGGAGGGGCCCGGCAGCGGCAGCCCCCGAGGTAAGGCGCCAGCTGCG
GTGGCAGAGAAGAGCCCTGTGCGAGTGCGGCCCCTGCGTGTCCTGGACGGCCCCGGGCCT
GCTGGGATGGCCGCCACATGCATGAAGTGTGTGGTGGGATCCTGCGCCGGCGTGAACACC
GGGGGCCTGCAGAGGGAGCGGCCACCCAGCCCGGGGCCTGCAAGCAGGCAGGCAGCCATT
CGCCAGCAGCCCCGGGCCCGGGCTGACTCACTGGGGGCCCCCTGCTGTGGCCTGGACCCT
CACGCTATCCCGGGGAGAAGCAGAGAGGCCCCCAAGGGTCCTGGGGCCTGGAGGCAGGGT
CCAGGCGGTAGCGGCTCCATGTCCTCGGACTCCAGCAGCCCAGACAGCCCGGGCATCCCC
GAAAGGTCCCCCCGCTGGCCTGAGGGTGCCTGCAGGCAACCGGGGGCCCTGCAGGGAGAG
ATGAGTGCCTTGTTTGCTCAAAAGCTGGAGGAGATCAGGAGTAAATCCCCCATGTTCTCC
GCCGGTAAGCCCCTCTTGCCCTGCGTGGTCCTCCCGCACGCCCCTGGCATGGCTGGGCCT
GGGTCACCTGCTGCTGCTTCTGCGTGGACGGTGTCGCCTCGTGTGCTCGTGCTCGTGGCT
CTGTATCCGTGGCACTGTCTCCGTGGCACTCTGCTCCCTTGGCTTGCCTGTGGCCCA

ORF Protein

Cloned ORF protein sequence for pFN21AF0417 Download

>FLJ00417  539 aa
MMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSK
GLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPFVEVE
IIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRT
LAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAA
GRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAP
EAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPLRVLDGPGPAGMAATCMKCVVGSCAGVNT
GGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQG
PGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFS
AGKPLLPCVVLPHAPGMAGPGSPAAASAWTVSPRVLVLVALYPWHCLRGTLLPWLACGP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AF0417 Download

>pFN21AF0417  6322 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGGCTCCCCCGACAGCCGGCCCCCTTCCTGG
CCCAGCTCTTCCGCCTGAGGACCCAGGGCCGGATCCGGAGAGCAGGTGGCTTTTCTTGAG
CGCCAACATTCTGCCCGTGGTGGAGCGGTGCATGGGTGCCATGCAAGAGGGGATGCAGAT
GGTGAAGCTGCGTGGCGGCTCCAAGGGCCTGGTCCGCTTCTACTACCTGGACGAGCACCG
CTCCTGCATCCGCTGGAGGCCCTCACGCAAGAACGAGAAGGCCAAGATCTCCATCGACTC
CATCCAGGAGGTGAGTGAGGGGCGGCAGTCGGAGGTCTTCCAGCGCTACCCTGACGGCAG
CTTCGACCCCTTTGTGGAGGTGGAGATCATTGGGCTCCCTGTGGACTGCAGCAGGGAGCA
GACCCGCGTGGTGGACGACAACGGGTTCAACCCCACCTGGGAGGAGACCCTGGTTTTCAT
GGTGCACATGCCGGAGATCGCGCTGGTCCGCTTCCTCGTCTGGGACCACGATCCCATTGG
GCGTGACTTCATTGGCCAGAGGACGCTGGCCTTCAGCAGCATGATGCCAGGCTACAGACA
CGTGTACCTAGAAGGGATGGAAGAGGCCTCCATCTTCGTGCATGTGGCTGTCAGTGACAT
CAGCGGTAAGGTCAAGCAGGCTCTGGGCCTAAAAGGCCTCTTCCTCCGAGGCCCAAAGCC
CGGCTCGCTGGACAGTCATGCTGCTGGGCGGCCCCCGGCCCGGCCCTCCGTTAGCCAGCG
GATCCTGCGGCGCACGGCCAGCGCCCCGACCAAGAGCCAGAAGCCGGGCCGCAGGGGCTT
CCCGGAGCTGGTCCTGGGTACACGGGACACAGGCTCCAAGGGGGTGGCAGACGATGTGGT
GCCCCCCGGGCCCGGACCTGCTCCGGAAGCCCCAGCCCAGGAGGGGCCCGGCAGCGGCAG
CCCCCGAGGTAAGGCGCCAGCTGCGGTGGCAGAGAAGAGCCCTGTGCGAGTGCGGCCCCT
GCGTGTCCTGGACGGCCCCGGGCCTGCTGGGATGGCCGCCACATGCATGAAGTGTGTGGT
GGGATCCTGCGCCGGCGTGAACACCGGGGGCCTGCAGAGGGAGCGGCCACCCAGCCCGGG
GCCTGCAAGCAGGCAGGCAGCCATTCGCCAGCAGCCCCGGGCCCGGGCTGACTCACTGGG
GGCCCCCTGCTGTGGCCTGGACCCTCACGCTATCCCGGGGAGAAGCAGAGAGGCCCCCAA
GGGTCCTGGGGCCTGGAGGCAGGGTCCAGGCGGTAGCGGCTCCATGTCCTCGGACTCCAG
CAGCCCAGACAGCCCGGGCATCCCCGAAAGGTCCCCCCGCTGGCCTGAGGGTGCCTGCAG
GCAACCGGGGGCCCTGCAGGGAGAGATGAGTGCCTTGTTTGCTCAAAAGCTGGAGGAGAT
CAGGAGTAAATCCCCCATGTTCTCCGCCGGTAAGCCCCTCTTGCCCTGCGTGGTCCTCCC
GCACGCCCCTGGCATGGCTGGGCCTGGGTCACCTGCTGCTGCTTCTGCGTGGACGGTGTC
GCCTCGTGTGCTCGTGCTCGTGGCTCTGTATCCGTGGCACTGTCTCCGTGGCACTCTGCT
CCCTTGGCTTGCCTGTGGCCCAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT